Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Up-regulation of Bax and Down-regulation of Bcl-2 in Oak Smoke Flavoring(Holyessing)-induced Apoptosis of Human Prostate Carcinoma Cells

참나무 목초액에 의한 전립선암세포의 apoptosis 유발기전에 관한 연구

  • Park Cheol (Department of Biochemistry, College of Oriental Medicine, Dongeui University) ;
  • Choi Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University) ;
  • Lee Won Ho (Department of Biology, College of Natural Sciences, Busan National University) ;
  • Choi Byung Tae (Department of Anatomy, College of Oriental Medicine, Dongeui University) ;
  • Lee Yong Tae (Department of Physiology, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicin) ;
  • Kim Gyeong Cheol (Department of Physiology, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicin)
  • 박철 (동의대학교 한의과대학 생화학교실) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 이원호 (부산대학교 자연과학대학 생물학과) ;
  • 최병태 (동의대학교 한의과대학 해부학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실 및 한의학연구소) ;
  • 김경철 (동의대학교 한의과대학 생리학교실 및 한의학연구소)
  • Published : 2003.02.01
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Abstract

We investigated the effects of Oak smoke flavoring (OSF, Holyessing) on the growth of DU145 and PC-3 human prostate carcinoma cells. OSF treatment resulted in a concentration-dependent growth inhibition in both DU145 and PC3 cell lines. The anti-proliferative effect of OSF treatment was associated with the induction of apoptotic cell death which was confirmed by morphological change such as membrane shrinking, rounding up and chromatin condensation in DU145 and PC-3 cells. DNA flow cytometry analysis confirmed that OSF treatment increased population of apoptotic sub-G1 phase. Furthermore, we observed an increase of pro-apoptotic protein Bax expression and a decrease of anti-apoptotic protein Bcl-2 by OSF treatment in a dose-dependent manner. OSF also induced a proteolytic cleavage of specific target proteins such as poly(ADP-ribose) polymerase (PARP) and β-catenin proteins. The present results indicated that OSF-induced inhibition of human prostate carcinoma cell proliferation is associated with the induction of apoptosis.

Keywords

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