Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study on the Effect of Jakyakgamcho-tang

작약감초탕의 효능에 관한 연구

  • Lee Yun Suk (Department of Pharmacology, School of Oriental Medicine, Wonkwang University) ;
  • Kim Hyung Chang (Department of Pharmacology, School of Oriental Medicine, Wonkwang University) ;
  • Whang Eui Hyun (Department of Pharmacology, School of Oriental Medicine, Wonkwang University) ;
  • Jo Sung Kyun (Department of Pharmacology, School of Oriental Medicine, Wonkwang University) ;
  • Lim In Gyu (School of Oriental Medicine, Professional Graduate School of Wonkwang University) ;
  • Han Jong Hyun (School of Oriental Medicine, Professional Graduate School of Wonkwang University)
  • 이윤석 (원광대학교 한의과대학 약리학교실) ;
  • 김형창 (원광대학교 한의과대학 약리학교실) ;
  • 황의현 (원광대학교 한의과대학 약리학교실) ;
  • 조성균 (원광대학교 한의과대학 약리학교실) ;
  • 임인규 (원광대학교 한의과전문대학원) ;
  • 한종현 (원광대학교 한의과전문대학원)
  • Published : 2003.04.01
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Abstract

Paeoniae Radix, Glycyrrhizae Radix and Jakyakgamchotang have been used in Oriental Medicine for many centuries as a treatment for various disease. The purpose of the present study is to determine the effect of Paeoniae Radix, Glycyrrhizae Radix and Jakyakgamchotang on narepinephrine(NE) induced contraction of isolated rabbit femoral artery. Rabbits (2.0kg, female) were killed by CO₂ exposure and a segment (8-10mm) of the aortic ring from each rabbit was cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 1.5g loading tension. The dose of NE which evoked 50% of maximal response (ED/sub 50/) was obtained from cumulative dose response curves for NE (10/sup -7/～10/sup -4/M). Contractions evoked by NE (ED/sub 50/) were inhibited significantly by Paeoniae Radix, Glycyrrhizae Radix and Jakyakgamcho-tang. The mean percent inhibition of NE induced contraction was 83.9% (p<0.01) after 150㎕/㎖ Paeoniae Radix, 101.1 %(p<0.01) after 150 ㎕/㎖, Glycyrrhizae Radix and 107.3%(p<0.01) after 150㎕/㎖ Jakyakgamcho-tang, Indomethacin slightly but significantly attenuated the inhibitory effects of Paeoniae Radix. Following treatment with indomethacin, the mean percent inhibition caused by 150㎕/㎖ Paeoniae Radix fell to 16.4% in femoral artery induced by NE contraction. Propranolol, ODQ, and L-NNA did not significantly alter the inhibitory effect of Paeoniae Radix. ODQ slightly but significantly attenuated the inhibitory effects of Glycyrrhizae Radix. Following treatment with ODQ, the mean percent inhibition caused by 150㎕/㎖ Glycyrrhizae Radix fell to 13.0% in femoral artery induced by NE contraction. Propranolol, indomethacin and L-NNA did not significantly alter the inhibitory effect of Glycyrrhizae Radix. L-NNA slightly but significantly attenuated the inhibitory effects of Jakyakgamchotang. Following treatment with L-NNA, the mean percent inhibition caused by 150㎕/㎖ Jakyakgamchotang fell to 13.8% in femoral artery induced by NE contraction. Propranolol, ODQ and indomethacin did not significantly alter the inhibitory effect of Jakyakgamcho-tang. These results indicate that Paeoniae Radix, Glycyrrhizae Radix and Jakyakgamcho-tang can relax NE induced contraction of the isolated rabbit femoral artery, and that this inhibition related to nitric oxide.

Keywords

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