Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Anti-inflammatory action by Gamisangryosamul-tang and The effect of Ziyang-Go on atopic dermatitis-like lesion and pruritus in NC/Nga mice

기미생료사물탕의 항염증효과와 지양고의 아토피피부염 손상 및 지양 효과에 미치는 영향

  • Kim Jeong Jin (Department of Pathology, School of Oriental Medicine, Kyunghee University) ;
  • Yang Sung Wan (Newcore OM Clinic, Seoul) ;
  • Son Nak Won (Department. of East-West Neuroscience, Graduate School of East-West medicine, Kyunghee University) ;
  • Ahn Kyoo Seok (Department of Pathology, School of Oriental Medicine, Kyunghee University)
  • 김정진 (경희대학교 한의과대학 병리학교실) ;
  • 양성완 (서울 서초구 뉴코아한의원) ;
  • 손낙원 (경희대학교 동서의학대학원 동서신경과학교실) ;
  • 안규석 (경희대학교 한의과대학 병리학교실)
  • Published : 2003.04.01
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Abstract

This research was performed to examine an anti-inflammatory effects of Gamisangryosamul-Tang(GSS) and anti-pruritus effects of Ziyang-Go(Salve). This study was processed by three experiments; Experiment 1: Inhibitory activity of GSS extract on the degranulation of mast cell and histamine release in plasma induced by compound 48/80 i.p, injection after the pretreatment of GSS extract i.p, injection in Sprague-Dawley rats, Experiment 2: Anti-inflammatory effect of GSS extract on macropharge raw 264,7 cells treated by LPS 250 ppm (before 2 hours). Experiment 3: Measurement of passive cutaneous anaphylaxis and atopic dermatitis using NC/Nga mice, GSS extract inhibited histamine release by 70% compared to compound 48/80 treated control group and histologically significantly reduced (P<0,01) the degranulation of mast cell in SD rats. In GSS extract treated group, the expression of TNF-α in macropharge cell showed the remarkable inhibitory effect about 62% (P<0,01) compared to LPS treated control group. The expression of IL-6 appeared more effective by 46% than the LPS treated control group and by 6% compared to hydrocortison treated group, Comparing with steroid (0.05% prednisolon) ointment, Ziyan-Go treated group showed the significant(30%) recovery on skin response index in atopic dermatis like anaphylaxis mice(NC/Nga), Finally, in scratching behavioral tests of NC/Nga mice for three weeks, Ziyang-Go treated group significantly (P<0.05) suppressed the pruritus on the face, neck, ears and dorsal skin than inbred NC/Nga mice. However, the change of IgE and IFN-γ from the spleen cell of NC/Nga mice was not significantly different between the oral intake of GSS extract group and of saline intaked control group. Summary and Conclusion: This study demons trates that Ziyang-go have the equal anti-pruritus effect to steroid ointment and GSS extract have the notable immunologic activity on inflammatory in vivo and in vitro model. Advanced experiment of this study will be required for more reliable information about the correlation between the lymphokine (i.e. IgE) and the anti-allergic effects of GSS.

Keywords

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