Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Glycyrrhizae Radix Water Extract on the Melanogenesis of Human Melanoma Cell

감초수추출물이 HM3KO 세포의 멜라닌 생성에 미치는 영향

  • Im Sook Jung (Department of Professional Graduate School of Oriental medicine, Wonkwang University) ;
  • Lim Nan Young (Department of Professional Graduate School of Oriental medicine, Wonkwang University) ;
  • Lee Sung Won (Department of Anatomy, School of Oriental medicine, Wonkwang University) ;
  • Kwak Gun Shin (Department of Professional Graduate School of Oriental medicine, Wonkwang University) ;
  • Ahn Sung Hun (Department of Professional Graduate School of Oriental medicine, Wonkwang University) ;
  • Mun Yeun Ja (Department of Professional Graduate School of Oriental medicine, Wonkwang University) ;
  • Woo Won Hong (Department of Anatomy, School of Oriental medicine, Wonkwang University)
  • 임숙정 (원광대학교 한의학전문대학원) ;
  • 임난영 (원광대학교 한의학전문대학원) ;
  • 이성원 (원광대학교 한의과대학 해부학교실) ;
  • 곽근신 (원광대학교 한의학전문대학원) ;
  • 안성훈 (원광대학교 한의학전문대학원) ;
  • 문연자 (원광대학교 한의학전문대학원) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실)
  • Published : 2003.04.01
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Abstract

This study was conducted to evaluate the effects of Glycyrrhizae Radix water extract, known as depigmenting agent, on melanin biosynthesis in the HM3KO human melanoma cells. The inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis was identified by mushroom tyrosinase assay in vitro. To determine whether Glycyrrhizae Radix water extract suppress melanin synthesis in cellular level, HM3KO cells were cultured in the presence of different concentrations of Glycyrrhizae Radix water extract and the effects on cell proliferation, melanin contents and tyrosinase activity were examined after 3 days. Treatment with Glycyrrhizae Radix at various concentrations did not exhibit any change of cell viability, and increased the cell proliferation. And the water extract of Glycyrrhizae Radix inhibited melanin contents and tyrosinase activity in a dose-dependent manner, compared with untreated group. These results suggest that the inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis is due to the suppression of tyrosinase in HM3KO cells.

Keywords

References

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