동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제17권2호
  • /
  • Pages.297-307
  • /
  • 2003
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

$CC1_4$로 유발된 백서의 간손상에 대한 갈근의 간보호작용 연구

The Study of Protective Effect of Puerariae Radix against $CC1_4$-induced Hepatotoxicity

  • 현동환 (동국대학교 한의과대학 병리학교실) ;
  • 정선영 (동국대학교 한의과대학 병리학교실) ;
  • 정상신 (동국대학교 한의과대학 병리학교실) ;
  • 하기태 (동국대학교 한의과대학 병리학교실) ;
  • 김철호 (동국대학교 한의과대학 생화학교실) ;
  • 김동욱 (목포대학교 생물산업학부 생약자원학과) ;
  • 김준기 (동국대학교 한의과대학 병리학교실) ;
  • 최달영 (동국대학교 한의과대학 병리학교실)
  • Hyun Dong Hwan (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Jung Sun Yeong (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Jung Sang Shin (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Ha Ki Tae (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Kim Cheorl Ho (Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Kim Dong Wook (Department of Medicinal Plant Resources, Mok-po National University) ;
  • Kim June Ki (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Choi Dall Yeong (Department of Pathology, College of Oriental Medicine, Dongguk University)
  • 발행 : 2003.04.01
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초록

In the present study, we investigated the protective effect of the Puerarie Radix water extract (PRE) against CCl₄-induced hepatotoxicity and the mechanism underlying these protective effects in the rats. The pretreatment of PRE has shown to possess a significant protective effect by lowering the serum alanine and aspartate aminoteansferase (AST and ALT) and alkaline phosphatase (ALP). This hepatoprotective action was confirmed by histological observation. In addition, the pretreatment of PRE prevented the elevation of hepatic malondialdehyde (MDA) formation and the depletion of reduced glutathione (GSH) content and catalase activity in the liver of CC1₄-injected rats. The PRE also displayed hydroxide radical scavenging activity in a dose-dependent manner (IC50 = 83.6 μg/ml), as assayed by electron spin resonance (ESR) spin-trapping technique. Moreover, the expression of cytochrome P450 2E1 (CYP2E1) mRNA, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR), was significantly decreased in the liver of PRE-pretreated rats when compared with that in the liver of control group. Based on these results, it was suggested that the hepatoprotective effects of the PRE may be related to antioxidant effects and regulation of CYP2E1 gene expression.

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