동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제17권3호
  • /
  • Pages.780-786
  • /
  • 2003
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

류마토이드 관절염 환자 골막세포에 대한 계혈등의 면역반응

Immunity Responses of the Spatholubus Suberectus Dunn to the Synovial Cells Isolated from Patients with Rheumatoid Arthritis

  • 서해경 (대전대학교 한의과대학 재활의학교실) ;
  • 오민석 (대전대학교 한의과대학 재활의학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Seo Hae Gyoung (Department of Rehabilitation, Collage of Oriental Medicine, Daejeon University) ;
  • Oh Min Suck (Department of Rehabilitation, Collage of Oriental Medicine, Daejeon University) ;
  • Kim Dong Hee (Department of Pathology, Collage of Oriental Medicine, Daejeon University)
  • 발행 : 2003.06.01
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초록

This study was carried out to know the immunity responses of the Spatholubus suberectus Dunn(hereinafter referred to STSD) to the synovial cells isolated from patients with rheumatoid arthritis. Various experiments were performed in vitro to analyse the immunity effects of STSD. Gene expression and production of pro-inflammatory cytokines such as IL-1β, IL-6, TNF-α, iNOS and COX-2 were determined by RT-PCR and ELISA kit. And also the binding activity of NF-kB and AP-1 were measured by Electromobility shift assay (EMSA) and the production of ROS was measured by flow cytometry. The results were obtained as follows 1. The gene expression and production of pro-inflammatory cytokines IL-1β, IL-6, TNF-α were reduced significantly. 2. The gene expression of iNOS and COX-2 were reduced. 3. The binding activity of NF-kB and AP-1 were inhibited. 4. The production of ROS in human synovial cells was reduced significantly. Comparison of the results for this study showed that STSD had immunomodulatory effects of suppressing or enhancing. So we expect that STSD should be used as a effective drugs for not only rheumatoid arthritis but also another auto-immune disease. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

키워드

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