Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Selaginella Tamariscina on Apoptosis via the Activation of Caspase-3 in HL-60

권백의 Caspase-3 활성화를 통한 HL-60 세포에서 세포사멸 유도효과

  • Nam Hang Woo (Department of Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Sung Won (Department of Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • An Byung Sang (Department of Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chough Won Joon (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Yeong Mok (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Mun Yean Ja (Department of Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Ahn Seong Hun (Department of Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo Won Hong (Department of Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Anatomy, College of Oriental Medicine, Wonkwang University)
  • 남항우 (원광대학교 한의학 전문대학원) ;
  • 이성원 (원광대학교 한의학 전문대학원) ;
  • 안병상 (원광대학교 한의학 전문대학원) ;
  • 조원준 (원광대학교 한의과대학 병리학교실) ;
  • 김영목 (원광대학교 한의과대학 병리학교실) ;
  • 문연자 (원광대학교 한의학 전문대학원) ;
  • 안성훈 (원광대학교 한의학 전문대학원) ;
  • 우원홍 (원광대학교 한의학 전문대학원, 원광대학교 한의과대학 해부학교실)
  • Published : 2003.06.01
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Abstract

In our previous studies, we reported that Selaginella Tamariscina(ST) induced apoptotic cell death in HL-60 cells selectively. The cell viability after treatment with extract of ST was quantified by MTT assay and trypan bleu exclusion method. The results showed that application with ST in HL-60 induced 40% cell death at the concentration of 400 ㎍/ml. The cancericidic effect of Selaginella Tamariscina was mediated by apoptosis. Thus, HL-60 cells exposed to Selaginella Tamariscina displayed the DNA fragmentation ladder and nucleus chromatin condensation characteristic for apoptosis. The enzyme activity of caspase-3 and actived caspase-3 protein were markedly increased in HL-60 cells treated with the extract of Selaginella Tamariscina. In addition, the extract of Selaginella Tamariscina induced cleavage of PARP, a known substrate for caspase-3. The expression of Bcl-2, anti-apoptotic protein, was decreased by treatment of the aqueous extract of Selaginella Tamariscina in a dose-dependent manner. And the expression of pro-apoptotic Bax protein was increased. In conclusion, our results suggest that the extract of Selaginella Tamariscina may induce the apoptotic death of HL-60 cells via activation of caspase-3, cleavage of PARP protein, depletion of cellular ATP levels and Bcl-2 degradation.

Keywords

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