Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Memory-enhancing Herbal Extract (YMT_02) on Modulating Pentraxin, PEP-19 and Transthyretin gene Expression in Rat Hippocampus

육미지황탕가미방에 의한 흰쥐 기억력 향상과 관련된 Hippocampus 부위의 특이 유전자 발현에 대한 연구

  • Sim Dea Sik (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Rho Sam Woong (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Lee Jin Woo (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Lee Eun A (Purimed R&D Institute) ;
  • Cho Chong Woon (Purimed R&D Institute) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyunghee University, Purimed R&D Institute) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Hong Moo Chang (Department of Physiology, College of Oriental Medicine, Kyunghee University)
  • 심대식 (경희대학교 한의과대학 생리학교실) ;
  • 노삼웅 (경희대학교 한의과대학 생리학교실) ;
  • 이진우 (경희대학교 한의과대학 생리학교실) ;
  • 이은아 (퓨리메드(주) 기업부설연구소) ;
  • 조종운 (퓨리메드(주) 기업부설연구소) ;
  • 배현수 (경희대학교 한의과대학 생리학교실, 퓨리메드(주) 기업부설연구소) ;
  • 신민규 (경희대학교 한의과대학 생리학교실) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실)
  • Published : 2003.06.01
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Abstract

The herbal extract(YMT_02) is a modified extracts from Yukmijihwang-tang(YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as replenishing yin and tonifying the kidneys herbal medicine for hundred years ia Asian countries. The purpose of this study is to: 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by passive avoidance test, 2) statistical evaluation of candidate gene expression (pentraxin. PEP-19, transthyretin) in rat hippocampus. The hippocampi of YMT_02 and control group were dissected and mRNA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Real Time PCR. The results were as follows : 1) passive avoidance test showed enhancing memory retentin by YMT_02 treatment, 2) expression of pentraxin, that accelerate degenerating of neuronal cell, was significantly decreased, 3) the mRNA of genes that has been known to be associated with protecting neuronal cell degeneration, such as PEP-19 and transthyretin, were significantly increased upon YMT_02 treatment. From above results, the administration of YMT_02 which tonify the function of Kidneys could enhance the ability of memory and learning. In addition, the administration of YMT_02 enhance memory retention through modulating particular gene (pentraxin, PEP-19, transthyretin) expressions in hippocampu.

Keywords

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