Apoptotic effect of Me fraction of Scutellaria barbata in human leukemic U937 cells

반지련의 Methyl chloride 분획이 U937 단핵 세포 암주의 세포고사에 미치는 영향

  • Cha Yun Yi (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee Eun Ok (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee Ju Ryoung (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kang In Cheol (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Park Young Doo (School of Biotechnology & Plant Metabolism Research Center, Kyunghee University) ;
  • Ahn Kyoo Seok (Oriental Medical College, Kyunghee University) ;
  • Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
  • 차윤이 (경희대학교 동서의학대학원) ;
  • 이은옥 (경희대학교 동서의학대학원) ;
  • 이주령 (경희대학교 동서의학대학원) ;
  • 강인철 (경희대학교 동서의학대학원) ;
  • 박영두 (경희대학교 생명공학연구원) ;
  • 안규석 (경희대학교 한의과대학) ;
  • 김성훈 (경희대학교 동서의학대학원)
  • Published : 2003.06.01

Abstract

Scutellaria barbata has been used as a traditional Chinese Herb for treating liver, lung and rectal tumors. In the present study, cytotoxic effect of Scutellaria barbata MC fradtion was investigated and it was found to inhibit proliferation of human leukemic U937 cells with an IC50 of approximately 10 μg/ml in a dose-dependent manner. We also demonstrated that Scutellaria barbata MC fraction caused apoptosis in U937 cells. In the flow cytometric assay, the MC fraction-treated U937 cells showed an increase in hypo-diplold Sub G1 DNA contents. DNA fragmentation was observed by TUNEL assay. An increase of Bax:Bcl-2 ratio, activation of caspase-9, caspase-3, and cleavage of poly (ADP-ribose) polymerase (PARP) were demonstrated by western blot analysis. Taken together, these results exerted that the MC fraction suppressed human leukemic U937 cell proliferation by inducing apoptosis via the mitochondrial pathway.

Keywords

References

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