Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Daejo-whan on the Ischemic Damage of Cerebral Neurons in Culture

대조환이 대뇌신경세포의 허혈성 손상에 미치는 영향

  • Park Se Hong (Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University) ;
  • Lee Kwang Ro (Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University) ;
  • Bai sun jun ;
  • Cheong Sang Su (Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University) ;
  • Kang Sei Young (Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University) ;
  • Lee Sang Kwan (Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University) ;
  • Lee Sung Keun (Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University) ;
  • Yoon Ji won (Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University) ;
  • Sung Kang Keyng (Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University)
  • 박세홍 (원광대학교 한의과대학 심계내과학교실) ;
  • 이광로 (원광대학교 한의과대학 심계내과학교실) ;
  • 배선준 (연세대학교 마취통증학교실) ;
  • 정상수 (원광대학교 한의과대학 심계내과학교실) ;
  • 강세영 (원광대학교 한의과대학 심계내과학교실) ;
  • 이상관 (원광대학교 한의과대학 심계내과학교실) ;
  • 이성근 (원광대학교 한의과대학 심계내과학교실) ;
  • 윤지원 (원광대학교 한의과대학 심계내과학교실) ;
  • 성강경 (원광대학교 한의과대학 심계내과학교실)
  • Published : 2003.12.01
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Abstract

This study was performed to clarify the neurotoxic mechanism of nerve cells damage by brain ischemia. The cytotoxic effect of ischemia was determined by XTT assay, NR assay, superoxide dismutase(SOD) activity, amount of malondialdehyde(MDA), lactate dehydrogenase(LDH) activity, protein synthesis and tumor necrosis factor(TNF)-α activities after cerebral neurons derived from mouse were exposed to ischemia for 1∼30 minutes. In addition, the protective effect of extract of Daejo-whan(DJW) on ischemia-induced neurotoxicity was examined in these cultures. 1. Ischemia decreased cell number and viability by XTT assay or NR assay when cultured cerebral neurons were exposed to 95% N2/5% CO₂ for 1∼20 minutes in these cultures. 2. Ischemia decreased SOD and protein syntheses, but it increased amount of MDA and, LDH and TNF-α activities in these cultures. 3. In the neuroprotective effect of DJW extracts on cerebral neurons damaged by ischemia, DJW extracts increased SOD activity and protein synthesis. While, it decreased amount of MDA and, LDH and TNF-α activities after cerebral neurons preincubated with herb extracts. It suggests that brain ischemia has neurotoxicity on cultured mouse cerebral neurons, and the herb extract such as DJW was very effective in blocking the neurotoxicity induced by ischemia in cultured mouse cerebral neurons.

Keywords

References

  1. Diabetes Care. v.19 Oxidative stress and diabetic vascular complications Dario, G.;Antonio, C.;Giuseppe, P.
  2. FASEB J. v.4 Role of oxygen free radicals in carcinogenesis and brain ischemia Floyd, R.A.
  3. J. Clin Invest. v.95 Modified low density lipoprotein and its constituents augment cytokine-activated vascular cell adhesion molecule-1 gene expression in human vascular endothelial cells Kham, B.V.;Pathasarathy, S.S.;Alexander, R.W.;Medford, R.M.
  4. J. Exp Neurol. v.124 Mechanisms of neurotrophic factor protection against calcium- and free radical mediated excitotoxic injury : Implications for treating neurogenerative disorders Mattson, M.P.;Cheng, B.;Smith-Swintosky, V.L.
  5. J. Physiol. v.394 Permeation and block of N-methyl-D-aspartic acid receptor channels by divalent cautions in mouse cultured central neurons Mayer, M.L.;Westbrook, G.L.
  6. J. Neurosci Res. v.37 Oxygen radical-induced neurotoxicity in spinal cord neuron cultures Michikawa, M.;Lim, K.T.;McLamon, J.G.;Kim, S.U.
  7. J. Immunol. Methods. v.65 Rapid colorimetric assay for cellular growth and survival : Application to proliferation and cytotoxic assays Mosmann, T.
  8. Hypertension. v.25 Hypertension and the pathogenesis of artherosclerosis. Oxidative stress and the mediation of arterial inflammatory response: A new perspective Alexander, R.W.
  9. J. Histochem. v.22 Cytochemical markers of ischemia in the heart and brain Borgers, M.;Vandeplassche, G.;van Reempts, J.
  10. Nature(London) v.336 NMDA receptors activate the arachidonic acid cascade system in striatal neurons Dumuis, A.;Sebben, M.;Haynes, L.;Pin, J.P.;Bockaert, J.
  11. J. Neurochem. v.59 Reactive oxygen species and the central nervous system Halliwell, B.
  12. J. Neurosci Res. v.25 Role of nerve growth factor in oxidant-antioxidant balanced and neuronal infury. Stimulation of hydrogen peroxide resistance Jackson, G.R.;Apfell, L.;Werrbach-Perex, K.;Perex-Polo, J.R.
  13. J. Tiss. Cult. Meth. v.9 A Simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/NR-90) Borenfreund, E.;Puerner, J.A.
  14. Chem. Pharm. v.35 no.1 Effect of Adriamycin on Cultured mouse embryo myocardial cells Takahashi, K.;Fujita, T.;Mayum, T.;Kish, T.
  15. 大韓韓醫學會誌 v.8 no.2 獨活類의 效能에 關한 實驗的 硏究 林泰榮
  16. J. Neurochem. v.51 Excitatory amino acid release from rat hippocampal slices as a consequence of free-radical formation Pellegrini-Giampietro, D.E.;Cherici, G.;Alesiani, M.;Carrla, V.;Moroni, F.
  17. J. Neuropathol. Appl Neurobiol. v.20 Excitatory amino acids, free radicals and the pathogenesis of motor neuron disease Zeman, S.;Lioyd, C.;Meldrum, B.;Leigh, P.N.
  18. 대한동의병리학회지 v.12 no.1 人蔘이 산소유리기로 손상된 척수신경세포의 손상에 미치는 영향 田炳薰;姜益賢;文炳淳
  19. Neurotoxicology. v.17 Methylmercury- induced neurotoxicity in cerebral neuron culture is blocked by antioxidants and NMDA receptor antagonists Park, S.T.;Lim, K.T.;Chung, Y.T.;Kim, S.U.
  20. Stroke v.14 A possible role of lipid peroxidation in cellular damages caused by cerebral ischemia and protective effect of alphatocopherol administration Yamamoto, M.;Scima, T.;Uozumi, T.;Yamada, K.;Kawasaki, T.
  21. 證脈方藥合編 黃度淵
  22. 明淸經驗方三種. 扶壽精方. 吳旻
  23. 요령중의잡지 v.10 河車大造丸治療男性不育症106例 鄧啓源
  24. 國譯醫學心悟 李源哲
  25. 本草綱目 李時珍
  26. 編註醫學入門(下卷) 李천
  27. 實用中風治療學 裵秉哲(外)
  28. 大韓韓方內科學會誌 v.13 no.1 虛勞의 治法 및 治方에 관한 文獻的 考察 白泰鉉
  29. 韓方成人病學會誌 v.1 흰쥐의 중대뇌동맥 폐쇄 후 大造丸 투여가 대뇌반구 및 시상위축에 미치는 영향 曺圭璇(外 2人)
  30. 大造丸이 흰쥐의 誘發된 高脂血症 및 적출심장에 미치는 영향 李知恩
  31. L-NAME으로 誘發된 학습·기억장애와 腦虛血 損傷에 관한 大造丸의 效果 金根宇
  32. 心系內科學 全國韓醫科大學 心系內科學敎室
  33. 劉河間三六書 劉完素
  34. 東垣十種醫書 李東垣
  35. 丹溪心法 朱丹溪
  36. 景岳全書 張景岳
  37. 醫林改錯 王淸任
  38. 實用中醫內科學 方藥中(外)
  39. 中醫急症大成 黃星垣(外)