Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inductive Effect of Scutellariae radix on Glutathione S-Transferase Yc1/2 from Rat Liver

황금이 백서의 간 조직 글루타치온 에스-전이 효고 Ycl/2의 발현 효과

  • Kim Young Sook (Department of Biochemistry, School of Medicine, Wonkwang University) ;
  • Kim Dong Hyun (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Choi Mi Jung (Department of Biochemistry, School of Medicine, Wonkwang University) ;
  • Kim Sung Min (Department of Biochemistry, School of Medicine, Wonkwang University) ;
  • Park Rae Kil (Department of Microbiology and Immunology, School of Medicine, Wonkwang University) ;
  • Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Bok Ryang (Department of Biochemistry, School of Medicine, Wonkwang University)
  • 김영숙 (원광대학교 의과대학 생화학교실) ;
  • 김동현 (한국과학기술연구원 생체대사 연구센터) ;
  • 최미정 (원광대학교 의과대학 생화학교실) ;
  • 김성민 (원광대학교 의과대학 생화학교실) ;
  • 박래길 (원광대학교 의과대학 미생물학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실) ;
  • 김복량 (원광대학교 의과대학 생화학교실)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The water extract of Scutellariae radix was treated to rat and the primary culture of hepatocytes, and the expressions of several glutathione S-transferase (GST) isozymes and the activity of GST Yc1/2 were investigated by Western blot and by the use of HPLC. The results were obtained as follows: The water extract of Scutellariae radix did not induce the expressions of cytosolic GST Ya and GST Yp in rat livers. But, the extract increased the expression of cytosolic GST Yc1/2 to 2-4 fold higher than control. The expression of GST Yc1/2 in the primary culture of rat hepatocytes was induced by the water extract of Scutellariae radix in a dose-dependent manner, reaching 21-fold over control with 50 ㎍/㎖ treatment. The induction of the expression of GST Yc1/2 in rat livers increased the formation of AFB₁-glutathione conjugate from AFB₁-8,9-epoxide which was made in the metabolism of AFB₁.

Keywords

References

  1. Adv. Cancer Res. v.29 The role of glutathione and glutathione S-transferases in the metabolism of chemical carcinogens and other electrophilic agents Chasseaud, L.F.
  2. Cancer Res. v.52 no.2 Complementary DNA cloning, messenger RNA expression, and induction of alpha-class glutathione S-transferases in mouse tissues Buetler, T.M.;Eaton, D.L.
  3. Carcinogenesis. v.18 no.8 Effect of naturally occurring coumarins on the formation of epidermal DNA adducts and skin tumors induced by benzo[a]pyrene and 7,12-dimethylbenz [a]anthracene in SENCAR mice Cai, Y.;Kleiner, H.;Johnston, D.;Dubowski, A.;Bostic, S.;Ivie, W.;DiGiovanni, J.
  4. Biochem. Biophys. Res. Commun. v.179 no.2 The in vivo disposition of aflatoxin B1 in rat liver Ewaskiewicz, J.I.;Devlin, T.M.;Ch'ih, J.J.
  5. Carcinogenesis. v.13 no.10 Inhibition of 7,12-dimethylbenz[a]anthracene- induced mammary tumors and DNA adducts by garlic powder Liu, J.;Lin, R.I.;Milner, J.A.
  6. Toxicol. Appl. Pharmacol. v.135 no.1 Induction of phase I and phase II drug-metabolizing enzyme mRNA, protein, and activity by BHA, ethoxyquin, and oltipraz Buetler, T.M.;Gallagher, E.P.;Wang, C.;Stahl, D.L.;Hayes, J.D.;Eaton, D.L.
  7. Hepatology. v.23 no.4 A comparison of the effect of inducers on the expression of glutathione-S-transferases in the liver of the intact rat and inhepatocytes in primary culture Langouet, S.;Morel, F.;Meyer, D.J.;Fardel, O.;Corcos, L.;Ketterer, B.;Guillouzo, A.
  8. Environ. Health Perspect. v.4 Chemoprevention by inducers of carcinogen detoxication enzymes Kensler, T.W.
  9. Cancer Epidemiol. Biomarkers Prev. v.6 no.4 Oltipraz chemoprevention trial in Qidong, People's Republic of China: study design and clinical outcomes Jacobson, L.P.;Zhang, B.C.;Zhu, Y.R.;Wang, J.B.;Wu, Y.;Zhang, Q.N.;Yu, L.Y.;Qian, G.S.;Kuang, S.Y.;Li, Y.F.;Fang, X.;Zarba, A.;Chen, B.;Enger, C.;Davidson, N.E.;Gorman, M.B.;Gordon, G.B.;Prochaska, H.J.;Egner, P.A.;Groopman, J.D.;Munoz, A.;Helzlsouer, K.J.;Kensler, T.W.
  10. Cancer Epidemiol. Biomarkers Prev. v.7 no.2 Oltipraz chemoprevention trial in Qidong, People's Republic of China: modulation of serum aflatoxin albumin adduct biomarkers Kensler, T.W.;He, X.;Otieno, M.;Egner, P.A.;Jacobson, L.P.;Chen, B.;Wang, J.S.;Zhu, Y.R.;Zhang, B.C.;Wang, J.B.;Wu, Y.;Zhang, Q.N.;Qian, G.S.;Kuang, S.Y.;Fang, X.;Li, Y.F.;Yu, L.Y.;Prochaska, H.J.;Davidson, N.E.;Gordon, G.B.;Gorman, M.B.;Zarba, A.;Enger, C.;Munoz, A.;Helzlsouer, K.J.(et al.)
  11. J. Natl. Cancer Inst. v.91 no.4 Protective alterations in phase 1 and 2 metabolism of aflatoxin B1 by oltipraz in residents of Qidong, People's Republic of China Wang, J.S.;Shen, X.;He, X.Zhu, Y.R.;Zhang, B.C.;Wang, J.B.;Qian, G.S.;Kuang, S.Y.;Zarba, A.;Egner, P.A.;Jacobson, L.P.;Munoz, A.;Helzlsouer, K.J.;Groopman, J.D.;Kensler, T.W.
  12. Mol. Pharmacol. v.49 no.5 Role of nitric oxide in the cytokine-mediated regulation of cytochrome P-450 Carlson, T.J.;Billings, R.E.
  13. Toxicol. Appl. Pharmacol. v.90 no.3 Comparative effects of butylated hydroxyanisole on hepatic in vivo DNA binding and in vitro biotransformation of aflatoxin B1 in the rat and mouse Monroe, D.H.;Eaton, D.L.
  14. Biochem. Mol. Biol. Int. v.43 no.4 Differential inhibition of aflatoxin B1 oxidation by gestodene action on human liver microsomes Kim, B.R.;Oh, H.S.;Kim, D.H.
  15. Methods in Enzymol. v.58 Measurement of growth and viability of cells in culture Patterson, M.K. Jr.
  16. Psychol. Rep. v.32 no.2 The KDs-1:validity, reliability and independence among symptom clusters for clinic and normal samples Wogan, M.;Amdur, M.;Kupfer, D.J.;Detre, T.P.
  17. Biochem.J. v.279 Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transgerase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide Hayes, J.D.;Judah, D.J.;McLellan, L.I.;Kerr, L.A.;Peacock, S.D.;Neal, G.E.
  18. Annu. Rev. Pharmacol. Toxicol. v.34 Mechanisms of aflatoxin carcino- genesis Eaton, D.L.;Gallagher, E.P.
  19. Planta. Med. v.66 no.1 Baicalein an in vitro antigenotoxic compound from Scutellaria baicalensis Lee, B.H.;Lee, S.J.;Kang, T.H.;Kim, D.H.;Sohn, D.H.;Ko, G.I.;Kim, Y.C.
  20. Biochim. Biophys. Acta. v.1472 no.3 Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Gao, Z.;Huang, K.;Yang, X.;Xu, H.