Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Ribavirin, Electric Current, and Shoot-tip Culture to Eliminate Several Potato Viruses

  • Yi Jung-Yoon (National Highland Agricultural Experiment Station, RDA) ;
  • Seo Hyo-Won (National Highland Agricultural Experiment Station, RDA) ;
  • Choi Young-Moo (National Highland Agricultural Experiment Station, RDA) ;
  • Park Young-Eun (National Highland Agricultural Experiment Station, RDA)
  • Published : 2003.06.01
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Abstract

To eradicate several viruses such as PVX, PVY, and PLRV which often cause considerable damages to the growth and yields of potatoes, several stems including shoot tips were excised from the potato plants grown for 50 days and electric shock was treated. Shoot tips excised from electric-shocked stems were transferred into the medium supplemented with antiviral compound, ribavirin to examine the combinatorial effect. When treated only with 20 mg/L ribavirin, PVX concentration in the regenerated plant-lets was slowly decreased as repeating sub-culture and finally, it took 32 weeks to reach completely PVX-free stock. With an electric shock treatment (10 mA electric current), all the replicates became free from PVY. However, PLRV was not completely eradicated from 94P70-4 and 93P29-3 lines even by treating with 10 mA electric shock. In this case, both electric shock and antiviral compound treatments in axillary buds from the stem segment were successful in eradicating viral contamination.

Keywords

References

  1. J Gen Virol v.34 Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses Clark MF;Adams AV https://doi.org/10.1099/0022-1317-34-3-475
  2. Potato Research v.39 Correlation of potato virus S and virus M contents of potato meristem tips with the percentage of virus-free plantlets produced in vitro Faccioli G;Colombarini A https://doi.org/10.1007/BF02358213
  3. Proc Wash State Potato Conf v.21 Producing virus-tested free potatoes by thermotherapy and meristem culture Farrell CJ;Martin MW;Thomas PE
  4. Agricell Report v.8 Electrical stimulation of tissue culture growth and morphogenesis Goldworthy A
  5. Phytopathology v.78 Potato virus elimination by heat and ribavirin Griffiths HM;Jack SA
  6. CIP Research Report Effects of chemical and heat therapy on virus concentration in in vitro potato plantlets Helen MG;Steven AS;John HD
  7. Tissue Culture Association Mannual v.3 no.1 Plant tissue culture media: Major constituents, their prepararion and some application Huang LC;Murashige T
  8. Amer Potato J v.59 Eradication of potato virus X from potato by ribavirin treatment of cultivated potato shoot tips Klein RE;Livingston CH https://doi.org/10.1007/BF02875354
  9. Amer Potato J v.62 The Pacific Northwest Potato Clonal Repository Kwiatkowski S;Martin MW;Thomas PE
  10. CIP Research Guide 3 Tissue culture for elimination of pathogens Lizarrago R;Panta A;Jayasinghe U;Dodds J
  11. Amer Potato J v.73 Electrotherapy and shoot tip culture eliminate potato virus X in potatoes Lozoya S;Abello H;Garcia R https://doi.org/10.1007/BF02853073
  12. Amer Potato J v.62 Kinetin, thermotherapy and tissue culture to eliminate potato virus X(PVX) in potato Lozoya SH;Madrigal-Vargas A https://doi.org/10.1007/BF02855604
  13. Amer Potato J v.61 Thermotherapy and tissue culture for elimination of potato virus X (PVX) in mexican potato cultivars resistant to late blight Lozoya SH;Merlin-Lara O https://doi.org/10.1007/BF02888976
  14. Amer Potato J v.59 The use of constant and alternating temperature and tissue culture to obtain PVS-free potato plants Lozoya SH;Dawson WO https://doi.org/10.1007/BF02889523
  15. Amer Potato J v.61 Development and maintenance of multi-disease resistant potato breeding parents Martin MW;Thomas PE;Santo GS;Pavek JJ;Corsini DL
  16. Physiol Veg v.14 Thiamine requirement of potato meristem tips cultured in vitro Pennazio S;Appiano A;Vecchiati M;D’Agostino G
  17. Ac Phytopathol Acad Scient Hung v.15 The use of electric current for obtaining mosaic-free almonds Quacquarelli A;Gallitelli D;Savino V;Piazzolla P
  18. Amer Potato J v.62 Eradication of potato virus Y and S from potato by chemotherapy of cultured axillary bud tips Wambugu FM;Secor GA;Gudmestad NC https://doi.org/10.1007/BF02853476