Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Selection and Characterizations of Gamma Radiation-Induced Submergence Tolerant Line in Rice

  • Lee In-Sok (Korea Atomic Energy Research Institute) ;
  • Kim Dong-Sub (Korea Atomic Energy Research Institute) ;
  • hua Jin (Department of Horticulture, Chungnam National Univ.) ;
  • Kang Si-Yong (Korea Atomic Energy Research Institute) ;
  • Song Hi-Sup (Korea Atomic Energy Research Institute) ;
  • Lee Sang-Jae (Korea Atomic Energy Research Institute) ;
  • Lim Yong-Pyo (Department of Horticulture, Chungnam National Univ.) ;
  • Lee Young-Il (Korea Atomic Energy Research Institute)
  • 발행 : 2003.09.01
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초록

The combination of a radiation technique with an in vitro culture system was appiled to develop submergence tolerant rice. The 3,000 $M_3$ lines with an average 80 percent of fertile grain were utilized for the selection of submergence tolerance. Salt tolerant lines were selected based on high plant height, root length and root number after submergence in plastic pots. Of the lines tested, the tolerant line (403-6) showed a dramatic difference in morphological traits under submergence compared to its original variety (Dongjinbyeo). It was suggested that genetic variations between the original variety and $M_3$-403-6 did exist. The levels of $\alpha$-amylase and alcohol dehydrogenase activities were significantly increased in the mutant line compared to its original variety. The mutant with greater tolerance showed less electrolyte leakage indicating a greater membrane integrity and better survival. Also, this line was much more resistant to a salt stress of $1.25\%$ than the original variety. The proline level of the line was significantly (p<0.01> higher than that of the original variety. The relationships between the inhibition of growth caused by stress and the physiological changes in the plant cell were discussed.

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참고문헌

  1. Crop Sci v.8 Testing rice seedlings for cold water tolerance Adair,C.R.
  2. Plant Physiol v.105 The response of maize seedlings of different ages to hypoxia and anoxic stress.Changes in induction of Adh 1 Mrna, ADH activity, and survival of anoxiz Andrews,D.L.;Drew,M.C.;Jhonson,J.R.;Cobb,B.G.
  3. Plant Soil v.39 Rapid determination of free proline for water-stress studies Bates,L.s.;Waldem,R.P.;Teare,I.D.
  4. Plant Biol v.1 Adaptations to flooding stress:From plant community to molecule Blom,C.W.P.M.
  5. J Exp Bot v.39 Hypoxia induces membrane depolarization and potassium loss from wheat roots but does not increase their permeability to sorbital Buwalda,F.;Thomson,C.J.;Steigner,W.;Barrett-Lennard,E.G.;Gibbs,J.;Greenway,H.
  6. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Brdafor,M.M.
  7. Sov Plant Physiol v.38 Method of determining plant resistance to oxygen deficiency Chirkova,T.V.;Zhukova,T.M.;Goncharova,N.N.
  8. Biochemistry and molecular biology of plants Carbohydrate metabolism. Dennis,D.T.;Blakeley,S.D.;Buchanan,B.B.(ed);Gruissem,W.(ed);Jones,R.L.(ed)
  9. Annu Rev plant Physiol Plant Mol Biol v.48 Oxygen dificiency and root metabolism:Injury and acclimation under hyposiz and anoxia Drew,M.C.
  10. Euphytica v.120 Mutations genetics of salt tolerance in barley:An assessment of golden promise and other semi-dwart mutants Forster,B.R.
  11. Fukutaku Y, Yamada Y (1984) Sources of proline nitrogen in water stressed soybean (Glycin max) 11. Fate of 15N-labled protein. Physiol Plant 61: 622-628 https://doi.org/10.1111/j.1399-3054.1984.tb05180.x
  12. Physiol Plant v.61 Sources of proline nitrogen in waterstressed soybean (Glycin max)Ⅱ.Fate of $^ {15}N$-labled protein Fukutaku,Y.;Yamada,Y.
  13. Plant Physiol v.31 Mechanism of salt tolerance in nonhalophytes Greenway,H.;Muns,R.
  14. Plant Physiol v.31 Amylolytic activities in cereal seeds under aerobic and anaerobic conditions Guglielminatti,L.J.;Yamaguchi,P.;Perata,P.;Alpi,A.
  15. Proc Int Seed Test Assoc v.25 Can we Measure Seedling vigor Heydecker,W.
  16. Plant Mol Biol v.36 Three cis-elements required for rice α-amylase Amy3D during sugar starvation Hwang,Y.S.;Karrer,E.E.;Thomas,B.R.;Chen,L.;Rodriguez,R.L.
  17. Plant Mol Biol v.40 Differential expression of rice α-amylase genes during seedling development under anoxia Hwang,Y.S.;Thomas,B.R.;Rodriguez,R.L.
  18. Crop Sci v.16 Rice seedling vigor at suboptimal temperature jones,D.B.;Peterson,M.L.
  19. J Exp Bot v.51 Abscisic acid and hypoxic induction of anoxiz tolerance in roots of lettuce seedlings Kato-noguchi,H.
  20. Plant Prod Sci v.5 Hypoxic induction of anoxiz tolerance in rice coleptiles Kato-Noguchi,H.
  21. Plant Cell Env v.3 Germination and seedling growth under anaerobic conditions in Echinochola crus-galli(Barnyard grass) Kennedy,R.A.;Barrett,S.C.H.;van der ZEE,D.;Rumpho,M.E.
  22. Plant Physiol v.100 Anaerobic metabolism in plants Kennedy,R.A.;Rumpho,M.E.;Fox,T.C.
  23. Gene v.94 Expression and secretion of rice α-amylase by Saccharomyces cerevisiae Kumagai,M.H.;Shah,M.;Therasima,M.;Vrkjan,Z.;Whitaker,J.R.;Rodriguez,R.L.
  24. Plant Physiol v.79 ABA accelerates adaptation of cultured tobacco cells to salt LaRosa,P.C.;Handa,A.K.;Hasegawa,P.M.;Bressan,R.A.
  25. Plant Cell Tiss Org Cult v.57 Improvement of rice callus regeneration in the presence of NaCl Lutts,S.;Kinet,J.M.;BouHarmont,J.
  26. Crop Sci v.33 Improved semidwarf rice lines with tolerance to submergence of seedlings Mackill,D.J.;Amante,M.M.;Vergara,S.;Sarkarung,S.
  27. Euphytica v.85 Application of in vitro mutation techniques for crop improvement Maluszynski,M.;Ahloowalia,B.S.;Sigurbjomsson,B.
  28. Plant Prod Sci v.5 Introduction of the long-coleoptile traits to improvement the establishment of directed rice in submergence fields in cool climates Mirua,K.;Kuroki,M.;Shimizu,H.;Ando,I.
  29. Plant Mutation Breedling for Crop Improvement v.2 In vitro mutation system for crop improvement Novak,F.J.
  30. Plant Physiol v.75 Proline and glycine betaine influence protein solvation Paleg,L.G.;Steward,G.R.;Bradler
  31. Proc Royal Soc Edinb v.102B Aspects of plant behaviour under anoxia and post anoxiz Pfister-Sleber,M.;Braendle,R.
  32. Ann Bot v.79(Suppl A) Mobilization of endersperm reserves in cereal seeds and anoxia Perata,P.;Guglielminetti,L.;Alpi,A.
  33. Planta v.144 Enzyme activites in concentrted solution of glycinebetaine and other solutes Pollard,A.;WynJones,R.G.
  34. Crop Sci v.26 Genetic variation for seedling vigor traits in rice Redona,E.D.;Mackill,D.J.
  35. Plant Physiol Biochem v.32 Plant metabolism under hypoxia and anoxia Ricard,B.;Couee,I.;Raymond,P.;Saglio,P.H.;Saint-Ges,V.;Pradet,A.
  36. Plant Physiol Biochem v.27 Anaerobic protein synthesis in different organs of germinating rice seeds Ricard,B.;Pradet,A.
  37. Japan J Breed v.39 Differences in seedling emergence and growth among rice (Oryza sative L.) ecospecies under reduced soil conditions Sasahara,T.;Ikarashi,H.
  38. Rep Hokkaido Perpect Agric Exp Stn v.24 Studies on bredling for the germinability at low temperature of rice varieties adapted to direct sowing cultivation in flooded paddy field in cool region Sasaki,T.
  39. Ann Bot v.74 Relationship between coleoptile elongation and alcoholic fementation in rice exposed to anoxiz.Ⅱ Setter,T.L.;Ella,E.S.;Valdes,A.P.
  40. Ann Bot v.79(Suppl) Physiology and genetics of submergence tolerance in rice Setter,T.L.;Ellis,M.;Laureles,E.V.;Ella,E.S.;Senadhira,D.;Mishra,S.B.;Sarkarung,S.;Dattas,S.
  41. Arch Biochem Biophys v.284 A quantitative assessment of the importance of barely seed α-amylase, β-amylase, debranching enzyme, and α-glucosidase in starch degradation Sun,Z.T.;Henson,C.A.
  42. Appl Microbiol v.43 Characterization of rice α-amylase isozymes expressed by Saccharomyces cerevisiae Therasima,M.;Katho,S.;Thomas,B.R.;Rodriguez,R.L.