Imaging Science in Dentistry

  • 제33권3호
  • /
  • Pages.143-149
  • /
  • 2003
  • /
  • 2233-7822(pISSN)
  • /
  • 2233-7830(eISSN)
대한영상치의학회 (Korean Academy of Oral and Maxillofacial Radiology)
권호 표지

방사선조사가 사람 정상 구강각화 세포의 세포주기, 세포사 및 수종 단백질의 발현에 미치는 영향

Effect of irradiation on cell cycle, cell death and expression of its related proteins in normal human oral keratinocytes

  • 강미애 (서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소) ;
  • 허민석 (서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소) ;
  • 이삼선 (서울대학교 치과대학 구강악안면방사선학교실, 치학연구소 및 BK21) ;
  • 오성욱 (서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소) ;
  • 이설미 (강릉대학교 치과대학 구강악안면방사선학교실) ;
  • 전인성 (인제대학교부속 상계백병원 치과학교실) ;
  • 최순철 (서울대학교 치과대학 구강악안면방사선학교실, 치학연구소 및 BK21) ;
  • 박태원 (서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소)
  • Kang Mi-Ae (Dept. of Oral and Maxillofacial Radiology and Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Heo Min-Suk (Dept. of Oral and Maxillofacial Radiology and Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Lee Sam-Sun (Dept. of Oral and Maxillofacial Radiology, Dental Research Institute, and BK21, College of Dentistry, Seoul National University) ;
  • Oh Sung-Ock (Dept. of Oral and Maxillofacial Radiology and Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Lee Sul-Mi (Dept. of Oral and Maxillofacial Radiology, College of Dentistry, Kangnung National University) ;
  • Jeon In-Seong (Dept. of Dentistry, Inje University, Sanggyepaik Hospital) ;
  • Choi Soon-Chul (Dept. of Oral and Maxillofacial Radiology, Dental Research Institute, and BK21, College of Dentistry, Seoul National University) ;
  • Park Tae-Won (Dept. of Oral and Maxillofacial Radiology and Dental Research Institute, College of Dentistry, Seoul National University)
  • 발행 : 2003.09.01
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초록

Purpose: To investigate the radiosensitivity of the normal human oral keratinocytes (NHOK), and the effect of irradiation on cell cycle and protein expression. Materials and Methods: To evaluate the radiosensitivity of NHOK, the number of colonies and cells were counted after irradiation and the SF2 (survival fraction at 2Gy) value, and the cell survival curve fitted on a linear-quadratic model were obtained. LDH analysis was carried out to evaluate the necrosis of NHOK at 1, 2, 3, and 4 days after 2, 10, and 20 Gy irradiation. Cell cycle arrest and the induction of apoptosis were analyzed using flow cytometry at 1, 2, 3, and 4 days after 2, 10, and 200y irradiation. Finally, proteins related cell cycle arrest and apoptosis were analysed by Western blot. Results: The number of survived cell was significantly decreased in a dose-dependent manner. The cell survival curve showed SF2, α, and β values to be 0.568, 0.209, and 0.020 respectively. At 200y irradiated cells showed higher optical density than the control group. After irradiation, apoptosis was not observed but G2 arrest was observed in the NHOK cells. 1 day after 10 Gy irradiation, the expression of p53 remained unchanged, the p2l/sup WAF1/Cipl/ increased and the mdm2 decreased. The expression of bax, bcl-2, cyclin B1, and cyclin D remained unchanged. Conclusion: These results indicate that NHOK responds to irradiation by G2 arrest, which is possibly mediated by the expression of p21/sup WAFl/Cipl/, and that cell necrosis occurs by high dose irradiation.

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