Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of the Artificial Culture Medium of Wild Ginsengs on Rumen Fermentation Characteristics In Vitro

산삼배양액 이용에 관한 반추위 미생물 대사 연구

  • Bae, G.S. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Nam, K.P. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Kim, H.S. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Lee, S.G. (Neobio Inc.) ;
  • Choi, H.S. (OOSOO.com Inc.) ;
  • Min, W.K. (Institute of Food Resource, Chung-Ang University) ;
  • Joo, J.W. (Department of Nutritional Resources, Konkuk University) ;
  • Maeng, W.J. (Department of Nutritional Resources, Konkuk University) ;
  • Chang, M.B. (Department of Animal Science & Technology, Chung-Ang University)
  • 배귀석 (중앙대학교 동물자원과학과) ;
  • 남경표 (중앙대학교 동물자원과학과) ;
  • 김혜숙 (중앙대학교 동물자원과학과) ;
  • 이상구 ((주)네오바이오) ;
  • 최행석 ((주)우수컴) ;
  • 민우기 (중앙대학교 식량자원연구소) ;
  • 주종원 (건국대학교 영양자원학과) ;
  • 맹원재 (건국대학교 영양자원학과) ;
  • 장문백 (중앙대학교 동물자원과학과)
  • Published : 2003.12.31
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Abstract

This study was conducted to determine the effects of the artificial culture medium of wild-ginsengs on in vitro fermentation characteristics. NH$_3$-N concentration was showed the highest in 3% WGM treatment among all treatments and control. In addition, microbial protein synthesis was significantly different in all treatments throughout the incubation time, and WGM 3% treatment was the highest at the 9 h incubation(P〈0.05). Protozoa numbers within rumen were decreased in all WGM treatments at 9 h incubation time, whereas WGM 3% treatment was always decreased throughout the incubation(P〈0.05). NDF and ADF digestibility were proportionally increased as the incubation time in both control and treatments. NDF digestibility showed no significantly difference between control and the 3% treatment, and ADF digestibility was similar in all. Total volatile fatty acid(VFA) concentrations of WGM treatments without 5% were significantly higher than control (P〈0.05). No differences were observed in total VFA, acetate, propionate and butyrate concentration among the WGM treatments. Acetate/Propionate ratio of WGM treatments was higher than control after 12 h incubation(P〈0.05). As a result of the artificial culture medium of wild-ginseng on rumen fermentation characteristics in vitro, microbial protein synthesis of WGM treatment was higher than control, and WGM 3% was the highest in all treatments(P〈0.05). The effect of saponin in artificial culture medium of wild-ginseng tended to decrease NH$_3$-N concentration, while it increases the microbial synthesis in early incubation. Therefore, artificial cultures medium of wild-ginseng can increase utilization of feed by microbial and anti-protozoal effects of saponin, which may enhance microbial synthesis capacity in early fermentation period in rumen.

본 실험은 수준별 산삼 배양액에 의한 반추위 내 미생물 발효성상에 미치는 영향에 대하여 조사하기 위해 실시되었다. 산삼 배양액의 수준에 따른 반추위 내 발효성상에 미치는 영향은 WGM을 3% 첨가한 처리구가 대조구 및 다른 WGM 수준 첨가 처리구에 비하여 미생물 단백질 합성량이 가장 높게 나타났다. 산삼 배양액 내에 존재하는 saponin의 영향으로 배양 초기 NH3-N 농도의 수준이 WGM를 첨가한 처리구가 대조구에 비해 낮은 경향(P〈0.05)을 보였으나, 미생물단백질 합성량은 WGM 처리구에서 6시간 이후 급격히 증가(P〈0.05)하는 경향을 나타내었다. 따라서 용해도가 높거나 급여 초기 사료의 이용율이 저하되는 급여 체계에서 산삼 배양액을 첨가는 초기 반추위 미생물의 이용율을 조절할 수 있을 것으로 사료된다. 반추위 내 프로토조아의 수는 WGM 처리구에서 전반적으로 배양 9시간까지 낮아졌고, 3% 처리구에서 가장 낮았는데(P〈0.05), 프로토조아 제거효과에 의해 반추위 미생물합성량 증진시키는 효과를 나타내었다. NDF와 ADF 소화율은 대조구와 모든 처리구에서 배양시간 경과에 따라 높아졌고, NDF 소화율은 대조구와 WGM 3% 처리구에서 그리고 ADF 소화율은 처리구별 차이가 없었다. Total VFA 생성량은 처리구가 대조구에 비해 낮았고, 배양 12시간부터는 5% 처리구에서 차이가 없게 나타났다. 따라서 용해도가 높거나 급여 초기 사료의 이용률이 저하되는 급여 체계에서 산삼 배양액을 첨가는 초기 반추위 미생물의 사료 이용률 증진과 프로토조아 제거효과에 의해 반추위 미생물합성량 증진에 도움이 될 수 있을 것이다.

Keywords

References

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