Isolation and antimicrobial susceptibility test of Mannheimia haemolytica from slaughtered cattle

도축우에서 Mannheimia haemolytica의 분리 및 항생제 감수성검사

  • Byun, Jae-Won (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Kim, Kyoung-Ho (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Lee, Seoung-Whan (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Lee, Hye-Wha (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Lee, Jung-In (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Hwang, Hyun-Soon (Incheon Metropolitan Health & Environmental Research Institute)
  • 변재원 (인천광역시보건환경연구원) ;
  • 김경호 (인천광역시보건환경연구원) ;
  • 이승환 (인천광역시보건환경연구원) ;
  • 이혜화 (인천광역시보건환경연구원) ;
  • 이정인 (인천광역시보건환경연구원) ;
  • 황현순 (인천광역시보건환경연구원)
  • Published : 2003.06.01

Abstract

This study was carried out to identify and investigate antimicrobial susceptibility for Mannheimuia haemolytical which is responsible for shipping fever. Samples were collected from nasal and lung of 100 adult healthy cattle which are slaughtered in Samsung meat corporation located in Incheon metropolitan city. lung lesion index have been investigated within 0-5 range according to Shewen and Willkie(Can J Vet Res 52:30-36, 1988). Eighty-seven of 100 cattle were under normal condition with 0-1 ranges. A total of 129 strains were collected from blood and tryptic soy agar. Among these strains, 100 strains were identified with Staphylococcus, Streptococcus and enterobacteria containing E coli. Biochemical and fermentation assay of arabinose, trehalose, xylose, mannose, mannitol, lactose and salicin were tested to identify with Mannheimia sp. for 7 strains shown haemolytic activity on blood agar. Five strains were identified with Mannheimia haemolytica and 2 strains were untyped. In seasonal survey, Mannheimia sp recovered from fall to winter(5 of 7) have been highly isolated rather than those from spring to summer(2 of 7). Mannheimiz haemolytica were susceptible to antibacterials tested in this study but more resistant to oxytetracycline and streptomycin.

Keywords

References

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