Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Tobacco mosaic virus Isolated fromSolanum tuberosum ‘Chubak’ in Korea

감자 '추백' 에 발생한 Tobacco mosaic virus 의 특성

  • Kim, Joung-Soo (Dept. of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jae-Hyun (Dept. of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Choi, Gug-Seoun (Dept. of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Chae, Soo-Young (Dept. of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Hyun-Ran (Dept. of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Joung, Bong-Nam (Dept. of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Choi, Yong-Mun (Dept. of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration)
  • 김정수 (농촌진흥청 원예연구소 원예환경과) ;
  • 김재현 (농촌진흥청 원예연구소 원예환경과) ;
  • 최국선 (농촌진흥청 원예연구소 원예환경과) ;
  • 채수영 (농촌진흥청 원예연구소 원예환경과) ;
  • 김현란 (농촌진흥청 원예연구소 원예환경과) ;
  • 정봉남 (농촌진흥청 원예연구소 원예환경과) ;
  • 최용문 (농촌진흥청 원예연구소 원예환경과)
  • Published : 2003.06.01
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Abstract

An isolate of Tobacco mosaic virus (TMV) was isolated from potato cultivar ‘Chubak’ showing vein clearing and mild mosaic at Namhae in Korea. This isolate, TMV-St, was differentiated from other tobamoviruses based on biological properties, serological relationships and nucleotide sequence analyses of coat protein genes. TMV-St caused typical symptoms on four indicator plants as compared to the tobamovirus of TMV-U1, Pepper mild mottle virus (PMMoV), and Tomato mosaic virus (ToMV), which caused economic losses in Solanaceous vegetables, tomato, pepper, and eggplant. Remarkably, the TMV-St induced distinctly different symptom of systemic chlorotic spots on Chenophodium murale. On C. murale, Gomphorena globosa, and Nic-otiana rustica, the four viruses were classed by the virulence of systemic or local infections. In serological test TMV-St antiserum showed a precipitation line with each other tabamovirus. The CP gene of TMV-St contain 477 nucleotides, and the nucleotides sequence was the most similar to that of TMV-U1.

남해지역의 원원종 종서 생산 포장에서 '추백' 품종에 나타난 엽맥투명 및 매우 약한 모자이크 증상을 나타내는 감자 잎에서 담배 모자이크 바이러스(TMV)를 분리하였다. 이 바이러스((TMV-St))는 생물학적, 혈청학적 유연관계 및 외피단백질의 염기서열 등을 통해 기존에 보고된 다른 tobamovires와 비교하였다. TMV-St는 5개의 지표식물 반응에서 토마토, 고추, 가지 등과 같은 가지과 작물에 경제적 피해를 주고 있는 TMV-U1, Pepper mild mottle virus(PMMoV) 및 Tomato mosaic virus(ToMV)와는 다른 기주 반응을 보였다. 특히 즙액접종에 의한 기주의 반응은 C.murale 접종엽과 상엽 모두에서 퇴록반점을 보였으며, C. murale, G. globosa, N.rustica 그리고 N. tabacum ce. Samsun nn 등 4가지 지표식물로 이들 바이러스 계통을 구분할 수 있었다. 혈청학적 검정에서 TMV-St는 TMV-U1, PMMoV 그리고 ToMV와의 반응에서 도두 뚜렷한 침강선을 형성하였다. TMV-St의 외피단백질은 477개의 염기서열로 되어 있으며, 이는 TMV-U1의 염기서열과 매우 유사하였다.

Keywords

References

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