Journal of the Optical Society of Korea

Optical Society of Korea (한국광학회)
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Conceptual Design of Soft X-ray Microscopy for Live Biological Samples

  • Kim, Kyong-Woo (X-ray Microscopy Research Center, Wonkwang University) ;
  • Nam, Ki-Yong (X-ray Microscopy Research Center, Wonkwang University) ;
  • Kwon, Young-Man (X-ray Microscopy Research Center, Wonkwang University) ;
  • Shim, Seong-Taek (X-ray Microscopy Research Center, Wonkwang University) ;
  • Kim, Kyu-Gyeom (X-ray Microscopy Research Center, Wonkwang University) ;
  • Yoon, Kwon-Ha (Department of Radiology, X-ray Microscopy Research Center, Wonkwang University School of Medicine)
  • Received : 2003.09.25
  • Published : 2003.12.01
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Abstract

This study describes the conceptual design of a soft x-ray microscope system based on a laserbased source for biomedical application with high resolution (${\leq}$50nm). The laboratory scale soft x-ray microscope consists of high power laser plasma x-ray source and grazing incidence mirrors with high reflectivity. The laser plasma source used for developing this system employs Q-switched Nd-YAG pulsed laser. The laser beam is focused on a tantalum (Ta) target. The Wolter type I mirror was used as condenser optics for sample illumination and as objective mirror for focusing on a detector. The fabrication of the Wolter type I mirror was direct internal cutting using ultraprecision DTM. A hydrated biological specimen was put between the two silicon wafers, the center of which was $Si_3N_4$ windows of 100㎚ thickness. The main issues in the future development work are to make a stable, reliable and reproducible x-ray microscope system.

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References

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Cited by

  1. Laboratory-size three-dimensional x-ray microscope with Wolter type I mirror optics and an electron-impact water window x-ray source vol.85, pp.9, 2014, https://doi.org/10.1063/1.4894468