지렁이 labial 유전자의 동정 및 머리재생 시 발현 양상에 관한 연구

Isolation of labial Gene in the Oligochaeta Perionyx excavatus and Expression Patterns during Head Regeneration

  • 조성진 (중앙대학교 생명과학과) ;
  • 이명식 (중앙대학교 생명과학과) ;
  • 탁은식 (중앙대학교 생명과학과) ;
  • 허소영 (중앙대학교 생명과학과) ;
  • 이종애 (중앙대학교 생명과학과) ;
  • 박범준 (중앙대학교 생명과학과) ;
  • 조현주 (중앙대학교 생명과학과) ;
  • 신주옥 (연세대학교 생명과학과) ;
  • 박순철 (중앙대학교 생명과학과)
  • 발행 : 2003.12.01

초록

Hox genes are pivotal in the control of morphogenesis along the anterior-posterior (AP) axis in various bilaterians. Several indications suggest their involvement in the control of cell growth and regeneration. For the labial full-length fragment, RACE-PCR was employed to obtain the 3' and 5' franking regions. Semi-quantitative RT-PCR analysis revealed that the labial expression began to increase at 12 hours after amputation. The peak expression was approximately 1.5-fold more than the unamputated controls. This result could give us information on the significance of Hox genes and the relationships between Hox genes during regeneration.

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