생쥐 초기 2-세포 배에서 세포 내 칼슘 농도의 변화에 $Ni^{2+}$이 미치는 영향

The effect of $Ni^{2+}$ on the intracellular $Ca^{2+}$ increase of the mouse early 2-cell embryos

  • 윤숙영 (성신여자대학교 자연과학대학 생물학과) ;
  • 이은미 (성신여자대학교 자연과학대학 생물학과) ;
  • 배인하 (성신여자대학교 자연과학대학 생물학과)
  • Yoon, Sook-Young (Department of Biology, College of Natural Sciences, Sungshin Women's University) ;
  • Lee, Eun-Mi (Department of Biology, College of Natural Sciences, Sungshin Women's University) ;
  • Bae, In-Ha (Department of Biology, College of Natural Sciences, Sungshin Women's University)
  • 발행 : 2003.12.30

초록

Objective: We reported the overcoming effect of $Ni^{2+}$ on the in vitro 2-cell block of mouse embryos. In this study, we aim to investigate whether $Ni^{2+}$ should induce intracellular $Ca^{2+}$ transient in the mouse embryos. Materials and Methods: Embryos were collected at post hCG 32hr from the oviduct of the ICR mouse and cultured in M2 medium omitted phenol red. Intracellular $Ca^{2+}$ was checked by using a confocal laser scanning microscope and fluo-3AM by using various intracellular $Ca^{2+}$ antagonists. Results: In 1mM $Ni^{2+}$ treated medium which contained $Ca^{2+}$(1.71mM), 75.7% of the embryos showed $[Ca^{2+}]i$ transient about 200 sec later. In the $Ca^{2+}$-free medium, 69.8% of the embryos showed $[Ca^{2+}]i$ transient. In U73122, phospholipaseC(PLC) inhibitor (5uM, 10min) pretreated group, 33.3% of the embryos showed $[Ca^{2+}]i$ transient. Heparine, inositol 1, 4, 5-triphosphate receptor(IP3R) antagonist preinjected embryos showed no response with 1mM $Ni^{2+}$. In danthrolene treatment, ryanodine receptor(RyR)-antagonist, 43% embryos showed $[Ca^{2+}]i$ transient but they showed delayed response about 340sec in the presence of $Ca^{2+}$. Conclusions: Summing up the above results, $Ni^{2+}$ seems to induce $Ca^{2+}$-release from the $Ca^{2+}$-store even in the $Ca^{2+}$-free medium. IP3 receptors of the mouse 2-cell embryos might have an essential role for the intracellular $Ca^{2+}$ increase by $Ni^{2+}$.

키워드

참고문헌

  1. Flach, G., M.H. Johnson, P.R. Braude, R.A.S. Tayler and V.N. Bolton, 1982. The transition from maternal to embryonic control in the 2-cell mouse embryo. EMBO 1: 681-686
  2. Braude P, Pelham H, Flach G, Lobatto R. 1979 Post-transcriptional control in the early mouse embryo. Nature. 282(5734): 102-5
  3. Goddard MJ, Pratt HP 1982. Control of events during early cleavage of the mouse embryo: an analysis of the '2-cell block'. J Embryol Exp Morphol 73: 111-33
  4. Muggleton-Harris, A., D.G. Whittingham and L. Wilson, 1982. Cytoplasmic control of preimplantation development in vitro in the mouse. Nature 299: 460-462 https://doi.org/10.1038/299460a0
  5. Whittingham, D.G., 1968. Development of zygotes in cultured mouse oviducts. I. The effect of varying oviductal conditions. J. Exp. Zool. 169: 391-398
  6. Ouhibi J Hamidi J Guillaud and Y Menezo 1990. Co-culture of 1-cell mouse embryos on different cell supports. Human Reprod. 5: 737-743
  7. Schini, S.A. and B.D. Bavister, 1986. Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biol. Reprod. 39: 1183-1192 https://doi.org/10.1095/biolreprod39.5.1183
  8. Chatot, C.L., C.A. Ziomek, B.D. Bavister, J.L. Lewis and I. Torres, 1989. An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J. Reprod. Fert. 86: 679-688
  9. Downs, S.M. and M.P.D. Dow, 1991. Hypoxanthine-maintained two-cell block in mouse embryos: Dependence on glucose and effect of hypoxanthine phospho-ribosyltransferase inhibitors. Biol. Reprod. 44: 1025-1039 https://doi.org/10.1095/biolreprod44.6.1025
  10. Nasr-Esfahani, M. H., J.R. Aitken and M.H. Johnson, 1990. Hydrogen peroxide levels in mouse oocytes and early cleavage stage embryos developed in vitro or in vivo. Development 109: 501-507
  11. Noda, Y., H. Matsumoto, Y. Umaoka, K. Tatsumi, J. Kishi and T. Mori 1991. Involvement of superoxide radicals in the mouse two cell block. Mol. Reprod. Dev. 28: 356-360 https://doi.org/10.1002/mrd.1080280408
  12. Kline D, Kline JT. 1992 Thapsigargin activates a calcium influx pathway in the unfertilized mouse egg and suppresses repetitive calcium transients in the fertilized egg. J Biol Chem. 267(25): 17624-30
  13. Umaoka, Y., Y. Noda, K. Narimoto and T. Mori, 1992. Effects of oxygen toxicity on early development of mouse embryos. Mol. Reprod. Dev. 31: 28-33 https://doi.org/10.1002/mrd.1080310106
  14. Santella L. 1998. The role of calcium in the cell cycle: facts and hypotheses. Biochem Biophys Res Commun. 244(2): 317-24 https://doi.org/10.1006/bbrc.1998.8086
  15. Whitaker, M and Patel R 1990. Calcium and cell cycle control. Development 108: 525-542
  16. Steinhardt RA, Alderton JM. 1988 Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo. Nature. 332(6162): 364-6
  17. Tombes, RM. and Borisy, GG. 1989. Intracellular free calcium and mitosis in mammalian cells: Anaphase onset is calcium modulated, but is not triggered by a brief transient. J. Cell Biol. 109: 627-636 https://doi.org/10.1083/jcb.109.2.627
  18. Kao JPY, Alderton JM, Tsien RY and SteinhsrdtRA 1990 Active involvement of $Ca^{2+}$ in mitotic progression of swiss 3T3 fibroblaaasts J Cell Biol 111: 183-196
  19. Homa ST. 1995 Calcium and meiotic maturation of the mammalian oocyte. Mol Re prod Dev. 40(1): 122-34. Review
  20. Hepler PK, 1989 Calcium transients during mitosis: observations in flux. J Cell Biol. 109(6 Pt 1): 2567-73
  21. Bae IH and Park JH : Studies on the requirements of $Ca^{2+}$ for cell division and $Ca^{2+}$ permeability of plasma membrane of fast dividing mouse embryo cells. Kor. J. Fert. Steril. 1987, 14: 93-100
  22. Abramczuk J, Solter D, Koprowski H 1977. The beneficial effect of EDTA on the development of mouse one-cell embryos in chemically defined medium. Dev. Biol. 61: 378-383 https://doi.org/10.1016/0012-1606(77)90308-6
  23. Suzuki, S., S. Komatsu, H. Kitai, Y. Endo, R. Iizuka and T. Fukasawa, 1988. Analysis of cytoplasmic factors in developmental cleavage of mouse embryo. Cell Differ. 24: 133-138 https://doi.org/10.1016/0045-6039(88)90064-4
  24. Fissore, R.A., K.V. Jackson and A.A. Kiessling, 1989. Mouse zygote development in culture medium without protein in the presence of ethylenediaminetetraacetic acid. Biol. Reprod. 41: 835-841 https://doi.org/10.1095/biolreprod41.5.835
  25. Bae IH and Yoon SY : The effect of $Ca^{2+}$ inhibitor on the in vitro 2-cell block of the mouse. Kor. J. Fert. Steril. 1995, 22: 1-10
  26. Berridge MJ. 1993 Cell signalling. A tale of two messengers. Nature. 365(6445): 388-9
  27. Hogan B, Beddington R, Constantini F, and Lacy E. 1986. Manipulating the mouse embryo(2nd Edition). Cold spring harbor laboratory press p390
  28. Moger WH. 1983 Effects of the calcium-channel blockers cobalt, verapamil, and D600 on Leydig cell steroidogenesis. Biol Reprod. 28(3): 528-35
  29. Wide M 1984 Effect of short-term exposure to five industrial metals on the embryonic and fetal development of the mouse. Environ Res. 33(1): 47-53
  30. Paksy K, Forgacs Z, Gati I. 1999 In vitro comparative effect of Cd2+, Ni2+, and CO2+ on mouse postblastocyst development. Environ Res. 80(4): 340-7
  31. Peres A. 1990. InsP3 and $Ca^{2+}$-induced $Ca^{2+}$-release in single mouse oocytes FEBS 275(1,2); 213-216
  32. Miyazaki S, Yuzaki M, Nakada K, Shirakawa H, Nakanishi S, Nakade S, Mikoshiba K. 1992 Block of $Ca^{2+}$ wave and $Ca^{2+}$ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertili zed hamster eggs. Science. 257(5067): 251-5
  33. Fujiwara T, Nakada K, Shirakawa H, Miyazaki S. 1993. Development of inositol trisphosphate- induced calcium release mechanism during maturation of hamster oocytes. Dev Biol. 156(1): 69-79 https://doi.org/10.1006/dbio.1993.1059
  34. Fissore RA, Robl JM. 1994. Mechanism of calcium oscillations in fertilized rabbit eggs. Dev Biol. 166(2): 634-42 https://doi.org/10.1006/dbio.1994.1343
  35. Mehlmann LM, Kline D. 1994. Regulation of intracellular calcium in the mouse egg: calcium release in response to sperm or inositol trisphosphate is enhanced after meiotic maturation. Biol Reprod 51(6): 1088-98 https://doi.org/10.1095/biolreprod51.6.1088
  36. Jones KT, carroll J and Whttiingham DG 1995 Ionomycin, thapsigargin, Ryanodine, and sperm induced $Ca^{2+}$-release increase during meioic maturation of mouse oocytes J Biol Chem 270(12); 6671-6677
  37. Yue C, White KN, Reed WA and Bunch TD 1995 The existence of inocitol 1,4,5-triphosphate and ryanodine receptors in mature bovine oocytes Development 121: 2645-2654
  38. Deng MQ, Huang XY, Tang TS and Sun FZ 1998 Spontaneous and fertilization-induced $Ca^{2+}$-oscilation in mouse immature germinal vesicle-stage oocytes Biol Reprod 58; 807-813
  39. Stachecki JJ, Armant DR. 1996 a Regulation of blastocoele formation by intracellular calcium release is mediated through a phospholipase C-dependent pathway in mice. Biol Reprod. 55(6): 1292-8
  40. Stachecki JJ, Armant DR. 1996 b Transient release of calcium from inositol 1,4,5-trisphosphatespecific stores regulates mouse preimplantation development. Development. 122(8): 2485-96
  41. Dupont G, McGuinness OM, Johnson MH,Berridge MJ, Borgese F. 1996 Phospholipase C in mouse oocytes: characterization of beta and gamma isoforms and their possible involvement in sperm-induced $Ca^{2+}$ spiking. Biochem J. 316 (Pt 2): 583-91
  42. Saunders CM, Larman MG, Parrington J, Cox LJ, Royse J, Blayney LM, Swann K, Lai FA. 2002 PLC zeta: a sperm-specific trigger of $Ca^{2+}$- oscillations in eggs and embryo development. Development. 129(15): 3533-44
  43. Ito J, Shimada M, Terada T. 2003 Effect of Protein Kinase C Activator on Mitogen-Activated Protein Kinase and p34cdc2 Kinase Activity During Parthenogenetic Activation of Porcine Oocytes by Calcium Ionophore. Biol Reprod. [Epub ahead of print].
  44. Sousa M, Barros A, Mendoza C, Tesarik J. 1996 Effects of protein kinase C activation and inhibition on sperm-, thimerosal-, and ryanodine-induced calcium responses of human oocytes. Mol Hum Reprod. 2(9): 699-708
  45. Nakao M, Ono K, Fujisawa S, Iijima T. 1999 Mechanical stress-induced $Ca^{2+}$ entry and Clcurrent in cultured human aortic endothelial cells. Am J Physiol. 276(1 Pt 1): C238-49
  46. He CL, Damiani P, Parys JB, Fissore RA. 1997 Calcium, calcium release receptors, and meiotic resumption in bovine oocytes. Biol Reprod. 57(5): 1245-5548
  47. Parrington J, Brind S, De Smedt H, GangeswaranR, Lai FA, Wojcikiewicz R, Carroll J. Expression of inositol 1,4,5-trisphosphate receptors in mouse oocytes and early embryos: the type I isoform is upregulated in oocytes and downregulated after fertilization. Dev Biol. 1998 203(2): 451-61
  48. Zhao F, Li P, Chen SR, Louis CF, Fruen BR 2001 Dantrolene inhibition of ryanodine receptor $Ca^{2+}$ release channels. Molecular mechanism and isoform selectivity. J Biol Chem. 276: 13810-6
  49. Bae IH, Channing CP. 1985 Effect of $Ca^{2+}$ on ping follicular oocyte maturation in vitro. Biol Reprod 33: 79-87
  50. parrish JJ, Kim Cl, Bae IH. 1992 Current Conceptes of cell-cycle regulation and its relationship to oocyte maturation, fertilization and embryo evelopement, Theriogenology 38: 277-296