대한한의학회지 (The Journal of Korean Medicine)

  • 제24권2호
  • /
  • Pages.159-165
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  • 2003
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  • 1010-0695(pISSN)
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  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지

SNAP 유도성 RINm5F 세포 독성에 대한 황연 추출물의 방어효과

Protective Effect of Coptidis Rhizoma on SNAP-Induced Cytotoxicity in Pancreatic RINm5F Cells

  • 류도곤 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 양정예 (전북대학교 구강생체과학연구소) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 김강산 (원광대학교 한의과대학 내과학교실)
  • 발행 : 2003.06.01
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초록

Objectives : Coptidis rhizoma (CR) is an oriental medicine that has been used in many traditional prescriptions against diabetes mellitus in Korea for centuries. Our purpose was to determine the protective effect and its action mechanism of CR on the cytotoxicity of pancreatic -cell line (RINm5F cell). Methods : In this experiment, we used methods such as MTT assay for detection of cytotoxicity, DNA fragmentation assay for detection of apoptotic cell death, LDH activity assay for detection of necrotic cell death, and measurement of $DiOC_{6}$ (3) retention for detection of mitochondrial membrane potential (MMP). Background : Nitric oxide (NO) is believed to playa key role in the process of pancreatic -cell destruction leading to insulin-dependent diabetes mellitus (IDDM). Results : Exposure of RINm5F cells to chemical NO donor such as S-nitroso-N-acetylpenicillamine (SNAP) induced cytotoxic events such as DNA fragmentation and lactate dehydrogenase (LDH) release into medium. However, pretreatment of RINm5F cells with CR extract ($10~50{\mu\textrm{g}}/ml$) for 3 hours prevented SNAP-induced DNA fragmentation and LDH release into medium through the inhibition of MMP disruption. Conclusions : These results suggest that CR may be a candidate for a therapeutic or preventing agent against IDDM.

키워드

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