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Analysis of Genetic Relatedness in Alternaria species Producing Host Specific Toxins by PCR Polymorphism

  • Kang, Hee-Wan (Graduate School of Bio and Information Technology, Hankyong National University) ;
  • Lee, Byung-Ryun (Crop Environment and Biotechnology, National Crop Experiment Station, Rural Development Administration (RDA)) ;
  • Yu, Seung-Hun (Department of Agricultural Biology, Chungnam National University)
  • 발행 : 2003.10.01

초록

Twenty universal rice primers (URPs) were used to detect PCR polymorphisms in 25 isolates of six different Alternaria species producing host specific toxins (HST). Eight URPs could be used to reveal PCR polymorphisms of Alternaria isolates at the intra- and inter-species levels. Specific URP-PCR polymorphic bands that are different from those of the other Alternaria spp. were observed on A. gaisen and A. longipes isolates. Unweighted pair-group method with arithmetic mean (UPGMA) cluster analysis using 94 URP polymorphic bands revealed three clustered groups (A. gaisen group, A. mati complex group, and A. logipes group).

키워드

참고문헌

  1. Adachi, Y., Watanabe, H., Tanabe, K., Doke, N., Nishimura, S. and Tsuge, T. 1993. Nuclear ribosomal DNA as a probe for genetic variation in Japanese pear pathotype of Alternaria alternata.Appl. Environ. Microbiol' 59:3197-3205
  2. Caetano-Anolles, G., Bassam, G. J. and Gresshoff, P. M. 1992. Primer-template interactions during DNA amplification fingerprinting with single arbitrary oligonucleotides. Moecul. General Genet. 235:157-165 https://doi.org/10.1007/BF00279356
  3. Go, S. J., Hong, S. B., Kang, H. W., Yu, S. H. and Ryu, J. C. 1997. Phylogenetic relationship of host-specific toxin producing Alternaria spp. on the basis of sequences of internal transcribed spacer in ribosomal DNA (in Korean). RDA Journal of Crop Protection 39: 1-9
  4. Jasalavich, C. A., Morales, V. M., Pelcher, L. E. and SeguinSwartz, G. 1995. Comparison of nuclear ribosomal DNA sequences from Alternaria species pathogenic to crucifers. Mycol. Res. 99:604-614 https://doi.org/10.1016/S0953-7562(09)80720-X
  5. Kang, H. W., Park, D. S., Go, S. J. and Eun, M. Y. 2001. Fingerprinting of diverse genomes using universal rice primers (URPs) generated from repetitive sequence of Korean weedy rice. Mol. Cells 13:281-287
  6. Kang, H. W., Park, D. S., Park, Y.J., Lee, B. M., Cho, S. M., Kim, K. T., Seo, G. S. and Go, S. J. 2002. PCR Based Detection of Phellinus linteus using Specific Primers Generated from Universal Rice Primer (URP) Derived PCR Polymorphic Band. Mycobiology 30:202-207 https://doi.org/10.4489/MYCO.2002.30.4.202
  7. Kang, H. W., Park, D. S., Park, Y. J., You, C. H., Lee, B. M., Eun, M. Y. and Go, S. J. 2001. Genomic differentiation among oyster mushroom (Pleurotus spp.) cultivars released in Korea by URP-PCR fingerprinting. Mycobiology 29:85-89
  8. Kim, B. R., Kang, H. W., Yu, S. H., Itoh, Y. and Kohmoto, K. 1998. RAPD analysis of host-specific toxins (HST) producing Alternaria species. Korean J. Plant Pathol. 14:92-98
  9. Kim, J. H., Lee, W. H., Ryu, Y. J., Cheong, S. S. and Choi, J. S. 2002. Analysis of genetic relationship and cultural characterization of Penicillium species isolated from postharvest decay of pear by random amplified polymorphic DNA The Korean J. Mycol. 3:78-85
  10. Kusaba, M. and Tsuge, T. 1994. Nuclear ribosomal DNA variation and pathogenic specialization in Alternaria fungi known to produce host specific toxins. Appl. Environ. Microbiol. 65:903-909
  11. Kusaba, M. and Tsuge, T. 1995. Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA Curro Genet. 28:491-498 https://doi.org/10.1007/BF00310821
  12. Morris, P. F., Connolly M. S. and St Clair, D. A. 2000. Genetic diversity of Alternaria alternata isolated form tomato in California assessed using RAPDs. Mycol. Res. 104:286-292 https://doi.org/10.1017/S0953756299008758
  13. Nishimura, S. and Kohmoto, K. 1983. Roles of toxins in pathogenesis. pp. 137-157. In: Toxins and Plant Pathogenesis, ed. by J. M. Daly and B. J. Deverall. Academic Press. Australia
  14. Otani, H. and Kohmoto, K. 1992. Host-specific toxins of Alternaria species. In: Alternaria: Biology, Plant Diseases and Metabolites, ed. by J. Chelkowski and A Visconti. pp. 123-156. Elsevier, Amsterdam
  15. Peever, T. L., Canihos, Y, Olsen, L., Ibanez, A., Liu, Y. C. and Timmer, L. W. 1999. Population genetic structure and host specificity of Alternaria spp. causing brown spot of Minneola tangelo and rough lemon in Florida. Phytopathology 89:851860 https://doi.org/10.1094/PHYTO.1999.89.10.851
  16. Pryor, B. M. and Gilbertson, R. L. 2000. Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences. Mycol. Res. 104:1312-1321 https://doi.org/10.1017/S0953756200003002
  17. Roberts, R. G., Reymond, S. T. and Andersen, B. 2000. RAPD fragment pattern analysis and morphological segregation of small-spored Alternaria species and species groups. Mycol. Res. 104: 151-160 https://doi.org/10.1017/S0953756299001690
  18. Rohlf, F.J. 2000. NTSYS-pc: Numerical taxonomy and multivariate analysis system.Yersion 2.1. Exeter Software, Setauket,NY
  19. Rotem, J. 1994. The genus Alternaria: Biology, Epidemiology, and Pathogenicity. APS Press, St Paul, MN
  20. Seo, G. S., Kim, B. R., Park, M. S., Kim, M. K. and Yu, S. H. 2002. Morphological characterization and URP-PCR analysis of Hypocrea sp., a weed mould of oyster mushroom cultivation. Korean J. Mycol. 3:86-94
  21. Simmons, E. G. 1992. Alternaria taxonomy: Current status, viewpoint, challenge. In: Alternaria-Biology, Plant Diseases and Metabolites. Topics in secondary metabolism Vol. 3, pp. 1-35. eds J. Chelkowski and A Visconti. Amsterdam: Elsevier
  22. Simmons, E. G. 1999, Alternaria themes and variations (236-243). Host-specific toxin producers. Mycotaxon 70:325-369
  23. Tsuge, T., Kobayashi, H. and Nishimura, S. 1989. Organization of ribosomal RNA genes in Alternaria alternate Japanese pear pathotype, a host-selective AK-toxin-producing fungus. Curro Genet. 16: 267-272 https://doi.org/10.1007/BF00422113
  24. Yu, S. H. 1992. Occurrence of Alternaria species in countries of the Far East and their taxonomy, pp. 37-62. In: J. Chelkowski and A Visconti (eds), Alternaria: biology, plant disease and metabolites. Elsevier, Amsterdam
  25. Weir, T. L., Huff, D. R., Christ, B. J. and Romaine, C. P. 1998. RAPD-PCR analysis of genetic variation among isolates of Alternaria solani and Alternaria alternata from potato and tomato. Mycologia 90:813-821 https://doi.org/10.2307/3761323
  26. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols. A guide to methods and applications, ed. by Innis, M. A., pp. 315-322. Academic Press, San Diego
  27. Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafaiski, J. A. and Tingey, S. V1. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535 https://doi.org/10.1093/nar/18.22.6531
  28. Wu, D. Y., Ugozzoli, L., Pal, B. K. and Qian, J. 1991. The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by polymerase chain reaction. DNA and Cell Biology 10:233-238 https://doi.org/10.1089/dna.1991.10.233

피인용 문헌

  1. Genetic Diversity of Paecilomyces japonica and Cordypces militaris Strains by URP-PCR Fingerprinting vol.39, pp.3, 2011, https://doi.org/10.4489/KJM.2010.39.3.180
  2. Genetic Diversity of Penicillium isolates Isolated from Pears with Postharvest Decay in Storage vol.40, pp.1, 2012, https://doi.org/10.4489/KJM.2012.40.1.011
  3. Genetic Divesity Analysis of Fungal Species by Universal Rice Primer (URP)-PCR vol.40, pp.2, 2012, https://doi.org/10.4489/KJM.2012.40.2.78