The Korean Journal of Ecology

The Ecological Society of Korea (한국생태학회)
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Effect of Cadmium on Oxidative Stress and Activities of Antioxidant Enzymes in Tomato Seedlings

  • Cho, Un-Haing (Department of Biology. Changwon National University) ;
  • Kim, In-Taek (Department of Biology. Changwon National University)
  • Published : 2003.06.01
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Abstract

Leaves of two-week old seedlings of tomato (Lycopersicon esculentum) were treated with various concentrations (0∼100 M) of $CdCl_2$ for up to 9 days and subsequent growth of seedlings, symptoms of oxidative stress and isozyme activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POX) were investigated. Compared with the non-treated control, Cd exposure decreased biomass but increased Cd accumulation, hydrogen peroxide production and lipid peroxidation as malondialdehyde (MDA) formation in leaves and roots. Further studies on the developmental changes of isozyme activities showed that Fe-SOD, Cu/Zn-SOD and one of three APX isozymes decreased and CAT and one of four POX isozymes increased in leaves, whereas Fe-SOD, one of three POX isozymes and two of four APX isozymes decreased and CAT increased in roots, showing different expression of isozymes in leaves and roots with Cd exposure level and time. Based on our results, we suggest that the reduction of seedling growth by Cd exposure is the oxidative stress resulting from the over production of $H_2O_2$ and the insufficient activities of antioxidant enzymes particularly involved in the scavenging of $H_2O_2$. Further, the decreased activities of SOD and APX isozymes of chloroplast origin, the increased activities of CAT and POX and high $H_2O_2$ contents with Cd exposure might indicate that Cd-induced oxidative stress starts outside chloroplast.

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References

  1. Plant Physiol. v.107 Dissection of oxidative stress tolerance using transgenic plants Allen,R.D. https://doi.org/10.1104/pp.107.4.1049
  2. Physiol. Plantarum. v.85 Ascorbate peroxidase a hydrogen peroxide scaveging enzyme in plants Asada,K. https://doi.org/10.1111/j.1399-3054.1992.tb04728.x
  3. New Phytol. v.127 Heavy metal accumulation and tolerance in British populations of the metallophyte Thlaspi caerulescens J. & C. Rresl (Brassicaceae) Baker,A.J.;R.D.Reeves;A.S.M.Hajar https://doi.org/10.1111/j.1469-8137.1994.tb04259.x
  4. J. Plant Nutr. v.13 Plant water relations as affected by heavy metal stress: A review Barcelo,J.;C.Poschenrieder
  5. Plant Sci. v.161 Water stress generates an oxidative stress through the induction of a specific Cu/Zn superoxide dismutase in Lotus corniculatus leaves Borsani,O.;P.Diaz;M.F.Agius;V.Valpuesta;J.Monza https://doi.org/10.1016/S0168-9452(01)00467-8
  6. Annu. Rev. Plant Phys. v.43 Sueroxide dismutase and stress tolerance Bowler,C.;T.Van Montagu;D.Inze https://doi.org/10.1146/annurev.pp.43.060192.000503
  7. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantity of protein utilizing the principle of protein-dye binding Bradford,M.M. https://doi.org/10.1016/0003-2697(76)90527-3
  8. Plant Sci. v.156 Mercury-induced oxidative stress in tomato seedlings Cho,U.H.;J.O.Park https://doi.org/10.1016/S0168-9452(00)00227-2
  9. Physiol. Plantarum v.82 Increased resistance to copper-induced damage of the root cell plasmalemma in copper-tolerant Silene cucubalus De Vos,C.H.R.;H.Schat;M.A.M. De Maal;R.Vooijs;W.H.O.Ernst https://doi.org/10.1111/j.1399-3054.1991.tb02942.x
  10. Plant Physiol. Bioch. v.31 Effect of copper on fatty acid composition and peroxidation of lipids in the roots of copper-tolerant and sensitive Silene cucubalus De Vos,C.H.R.;W.M.Ten Boukum;R.Vooijs;H.Schat;L.J.De Kok
  11. J. Exp. Bot. v.32 Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase Dhindsa,R.S.;P.Dhindsa;T.A.Thorpe https://doi.org/10.1093/jxb/32.1.93
  12. Ascorbic acid; in Antioxidants in Higher Plants Foyer,C.H.;R.C.Alscher(ed.);J.L.Hess(ed.)
  13. Physiol. Plantarum v.92 Photooxidative stress in plants Foyer,C.H.;M.Lelandais;K.J.Kunert https://doi.org/10.1111/j.1399-3054.1994.tb03042.x
  14. Arch Biochem. Biophys. v.247 Biological effects of the superoxide radical Fridovich,I. https://doi.org/10.1016/0003-9861(86)90526-6
  15. Biochem. J. v.219 Oxygen toxicity, oxygen radicals, transition metals and disease Halliwell,B.;J.M.C.Gutteridge https://doi.org/10.1042/bj2190001
  16. Free Radicals in BIology and Medicine Halliwell,B.;J.M.C.Gutteridge
  17. Physiol Plantarum v.61 Absorption and translocation of Cd in bush beans ( Phaseolus vulgaris0 Hardiman,R.T.;B.Jacoby https://doi.org/10.1111/j.1399-3054.1984.tb05189.x
  18. Cal. Agri. Exp. Station. Circular v.347 The water culture method for growing plants without soil Hoagland,D.R.;D.I.Amon
  19. Science v.240 DNA damage and oxygen radical toxicity Imlay,J.A.;S.Linn https://doi.org/10.1126/science.3287616
  20. Plant Physiol. v.116 Oxidative damage in pea plants exposed to water deficit or paraquat Iturbe-Ormaetxe,I.;P.R.Escuredo;C.Arrese-lgor;M.Bacana https://doi.org/10.1104/pp.116.1.173
  21. Biochem. Biophys. Acta. v.440 The effect of hydrogen peroxide on CO₂fixation of isolated intact chloroplast Kaiser,W.
  22. Free Radical. Res. v.31 Differential gene expression of rice superoxide dismutase isoforms to oxidative and environmental stresses Kamikana,H.;S.Morita;M.Tokumoto;T.Masamura;K.Tanka https://doi.org/10.1080/10715769900301541
  23. Eur. J. Forest. Pathol. v.4 The use of peroxidase activity for monitoring and mapping air pollution areas Keller,T. https://doi.org/10.1111/j.1439-0329.1974.tb00407.x
  24. Physiol. Plantarum v.36 Effect of Cd on respiration rate and activity of several enzymes in soybean seedlings Lee,K.C.;B.A.Cunningham;G.M.Paulsen;G.H.Liang;R.B.Moore https://doi.org/10.1111/j.1399-3054.1976.tb05017.x
  25. Plant Sci. v.159 Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays Lee,D.H.;C.B.Lee https://doi.org/10.1016/S0168-9452(00)00326-5
  26. J. Plant Nutr. v.16 Response of leguminosae to cadmium exposure Leita,L.;M. De Nobili;C.Mondini;M.T.Baca-Garcia https://doi.org/10.1080/01904169309364670
  27. Handbook of detection of enzyme on electrophoresis gels Manchenko,G.P.
  28. Plant Sci. v.127 Response of antioxidant enzymes to excess copper in tomato (Lycopersicon esculentum Mill) Mazhoudi,S.;A.Chaoui;M.H.Ghorbal;E.E.Ferjani https://doi.org/10.1016/S0168-9452(97)00116-7
  29. Plant Physiol. v.122 Iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance McKersie,B.D.;J.Murnaghan;K.S.Jones;S.R.Bowley https://doi.org/10.1104/pp.122.4.1427
  30. Plant J. v.5 Regulation of pea cytosolic ascorbate and other antioxidant enzymes during the progression of drougth stress and following recovery from drought Mittler,R.;A.Zilinskas https://doi.org/10.1111/j.1365-313X.1994.00397.x
  31. Plant J. v.4 Hydrogen peroxide and lignification Olson,P.D.;J.E.Varner https://doi.org/10.1046/j.1365-313X.1993.04050887.x
  32. Plant Cell Physiol. v.35 The influence of apoplastic ascorbate on the activities of cell-wall associated peroiddase and NADH-oxidases in needles of Norway spruce (Picea abies L.) Otter,T.;A.Polle https://doi.org/10.1093/oxfordjournals.pcp.a078717
  33. Phytochemistry v.45 Cadmium-and copper-induced changes in tomato membrane lipids Ouariti,O.;N.Boussama;M.Zarrouk;A.herif;M.H.Ghorbal https://doi.org/10.1016/S0031-9422(97)00159-3
  34. Oxidative Stress and the Molecular Biology of Antioxidants Defense Defense against photooidative damage in plants Polle,A.;J.Scandalios(ed.)
  35. Plant Cell v.6 Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide Prasad,T.K.;Anderson,M.D.;B.A.Martin;C.R.Stewart https://doi.org/10.1105/tpc.6.1.65
  36. Environ. Exp. Bot. v.44 Changes in peroxidase activity and isozymes in spruce needles after exposure to different concentrations of cadmium Radotic,K.;T.Ducic;D.Mutavdzic https://doi.org/10.1016/S0098-8472(00)00059-9
  37. Plant Physiol. v.85 Compartmental efflux and removal of extracellular cadmium from roots Rauser,W.E. https://doi.org/10.1104/pp.85.1.62
  38. Ann. Bot. v.82 Hydrogen peroxide production in a general property of the ligifying xylem from vascular plants Ros-Barcelo,A. https://doi.org/10.1006/anbo.1998.0655
  39. Plant Physiol. Bioch. v.40 Developmental regulation of the H₂O₂-producing system and of a basic peroxidase isoenzyme in the Zinnia elegans lignifying xylem Ros-Barcelo,A.;F.Pomar;M.Lopez-Serrano;P.Martinez;M.A.Pedreno https://doi.org/10.1016/S0981-9428(02)01376-1
  40. Physiol. Plantarum v.72 Toxic oxygen species and protective systems of the chloroplast Salin,M.L.
  41. Environ. Exp. Bot, v.41 Responses to dadmium in higher plants Sanita di Toppi,L.;R.Gabbrielli https://doi.org/10.1016/S0098-8472(98)00058-6
  42. Physiol. Plantarum v.87 Inhibition of eletron flow around photosystem I in chloroplasts of Cd-treated maize plants is due to Cd-induced iron defieiency Siedlecka,A.;T.Baszynski https://doi.org/10.1111/j.1399-3054.1993.tb00142.x
  43. Physiol. Plantarum v.85 Phytoxicity of cadmium ions on germinating seedlings of mung bean ( Phaseolus vulgaris): involvement of lipid peroxides in chlorophyll degradation Somashekaraiah,B.V.;K.Padmaja;A.R.K.Prasad https://doi.org/10.1111/j.1399-3054.1992.tb05267.x
  44. Plant. Sci. v.161 Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L) under NaCl salinity Sudhakar,C.;A.Lakshmi;S.Giridarakumar https://doi.org/10.1016/S0168-9452(01)00450-2
  45. New Phytol. v.105 The role of free radicals in senescence and wounding Thompson,J.E.;R.L.Legge;R.L.Barber https://doi.org/10.1111/j.1469-8137.1987.tb00871.x
  46. Plant Cell Environment v.13 Effects of metals on enzyme activity in plants Van Assche,F.;C.P.H.Clijsters https://doi.org/10.1111/j.1365-3040.1990.tb01304.x

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