KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation and identification of lignans as Antioxidant from loaves of Catalpa ovata G. $D_{ON}$

개오동나무 잎으로부터 항산화 활성을 갖는 lignan 화합물의 분리 및 동정

  • 국주희 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 마승진 ;
  • 문제학 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 박근형 (전남대학교 식품공학과 및 농업과학기술연구소)
  • Published : 2003.12.01
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Abstract

The methanol extract from leaves of Catalpa ovata 6. DoN showed DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, and its antioxidative compounds were studied. The ethyl acetate-soluble neutral fraction from the methanol extract was successively purified with silica gel adsorption column chromatography, Sephadex LH-20 column chromatography and HPLC. Three antioxidative compounds were isolated and identified as piperitol, pinoresinol and lariciresinol by HR-MS and NMR spectroscopic analyses. The DPPH radical-scavenging activity of the identified compounds decreased in the order of lariciresinol > pinoresinol > piperitol.

개오동나무 (Catalpa ovata G. Don) 잎의 MeOH 추출물은 DPPH 라디칼 소거활성을 나타냈으며, 이에 개오동나무 잎에 함유된 항산화물질을 구명하고자 하였다. MeOH 추출물을 용매분획하여 EtOAc 가용 중성획분을 얻고, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography로 정제한 다음 HPLC에 의해 3종의 활성물질을 단리하였다. 단리된 물질들은 HR-MS, 1H-NMR, 13C-NMR, 2D-NMR 등의 기기분석에 의해 piperitol, pinoresinol, lariciresinol로 동정하였다. 이들 물질들을 대상으로 DPPH 라디칼 소거활성을 조사한 결과, lariciresinol ($SC_{50}$/, 19$\mu\textrm{g}$/mL) ＞ pinoresinol ($SC_{50}$/, 31$\mu\textrm{g}$/mL) ＞ piperitol ($SC_{50}$/, 59$\mu\textrm{g}$/mL)의 순으로 항산화활성이 나타났다.

Keywords

References

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