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The Pharmaceutical Society of Korea (대한약학회)
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Detection and Characterization of 45 kDa Platelet Activating Factor Acetylhydrolase in Cerebrospinal Fluid of Children with Meningitis

  • Moon, Tae-Chul (College of Pharmacy, Yeungnam University) ;
  • Kim, Mi-Suk (College of Pharmacy, Yeungnam University) ;
  • Lee, Su-Jeong (Department of Microbiology, College of Medicine, Yeungnam University) ;
  • Lee, Tae-Yoon (Department of Microbiology, College of Medicine, Yeungnam University) ;
  • Kwon, Soon-Hak (Department of Pediatrics, Kyungpook National University Hospital) ;
  • Baek, Suk-Hwan (Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University) ;
  • Chang, Hyeun-Wook (College of Pharmacy, Yeungnam University)
  • Published : 2003.07.01
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Abstract

Platelet activating factor acetylhydrolase (PAF-AH) activity has been identified in cerebrospinal fluid (CSF) samples taken from children with meningitis. We reported that PAF-AH activity is significantly increased, by about 3 fold, in patients with meningitis compared to control subjects. Because of limited knowledge about this enzyme in CSF, we examined the biochemical properties of CSF PAF-AH. PAF-AH of CSF was calcium independent, showed a broad pH spectrum and was relatively heat stable. In addition, this enzyme activity was strongly inhibited by phenylmethanesulfonyl fluoride (PMSF), partially inhibited by p-bromophenacylbromide (p-BPB), uninhibited by iodoacetamide, and moderately stimulated by dithiothreitol (DTT). PAF-AH of CSF did not degrade phospholipid with a long chain fatty acyl group at sn-2 position. This enzyme hydrolyzed PAF and oxidatively modified phosphatidylcholine. Furthermore, we identified a monomeric polypeptide with a molecular weight of approximately 45 kDa by Western blot using human plasma PAF-AH antibody. These results suggested that plasma type PAF-AH activity exist in CSF taken from children with meningitis.

Keywords

References

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