Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Advanced Lipid Extraction Method for the Determination of the Phospholipase D Activity

  • Yon, Chang-Suek (Institute of Biomedical Science and Department of Biochemistry, College of Medicine, Hanyang University) ;
  • Lee, Min-Ho (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Oh, Doo-Yi (Institute of Biomedical Science and Department of Biochemistry, College of Medicine, Hanyang University) ;
  • Kim, Dal-Soo (Department of Neurosurgery, Uijongbu St. Mary′s Hospital, The Catholic University of Korea, College of Medicine, PaiChai University) ;
  • Lee, Ki-Sung (The Research Center for Bio-Medicinal Resources, PaiChai University) ;
  • Han, Joong-Soo (Institute of Biomedical Science and Department of Biochemistry, College of Medicine, Hanyang University, The Research Center for Bio-Medicinal Resources, PaiChai University)
  • Published : 2003.06.01
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Abstract

Phospholipase D is a ubiquitous enzyme that plays an important role in various lipid mediated cellular signaling pathways and produces rare phospholipids, phosphatidylethanol or phosphatidylbutanol, instead of phosphatidic acid with unique catalytic activity transphosphatidylation in the presence of primary alcohols. The reaction products, phosphatidylethanol or phosphatidylbutanol are used as markers of in vitro phospholipase D activity in many studies. For the sensitive detection of the phospholipase D products, we developed an advanced lipid extraction method that facilitates recovery of the compounds. With the new method, the activity change of phosaholipase D by agonists could be detected more easily and the recovery rate was also increased. The increase of detected enzyme activity change was about double fold compared to the conventional lipid extraction method. This method provides selective force for the phospholipase D products in the extraction procedure.

Keywords

References

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