Identification of Endogenous Gibberellins by Feeding of [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ in Chinese Yam, Dioscorea opposite

  • Lee, In-Jung (College of Agricultural Life Sciences, Division of Plant Biosciences, Kyungpook National University) ;
  • Kim, Sang-Kuk (Institute for Bioresources Research, Kyongbuk Provincial Agricultural Technology Administration) ;
  • Lee, Sang-Chul (College of Agricultural Life Sciences, Division of Plant Biosciences, Kyungpook National University) ;
  • Lee, Bong-Ho (Institute for Bioresources Research, Kyongbuk Provincial Agricultural Technology Administration) ;
  • Jang, Soo-Won (College of Agricultural Life Sciences, Division of Plant Biosciences, Kyungpook National University) ;
  • Park, Tae-Shik (Division of Rice Physiology and Cultivation, National Crop Experiment Station, Rural Development Administration)
  • 발행 : 2003.06.01

초록

The metabolism of [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ in the Chinese yam (Dioscorea opposita Thunb. var. Tsukune) was examined to determine the identification of endogenous gibberellins. [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ was metabolized to $\textrm{GA}_{53}$, $\textrm{GA}_{44}$, $\textrm{GA}_{19}$, $\textrm{GA}_{20}$, $\textrm{GA}_1$, $\textrm{GA}_8$, $\textrm{GA}_{15}$, $\textrm{GA}_{24}$, $\textrm{GA}_9$, $\textrm{GA}_{36}$ and $\textrm{GA}_4$. Radioactivity of GAs in non C-13 hydroxylation route was five-fold higher than that of early C-13 hydroxylation in analyzed GA-metabolites. Radioactivity of $\textrm{GA}_4$ was always four times higher than that of $\textrm{GA}_1$ at every feeding time. $\textrm{GA}_1$ radioactivity has always a lower level to below 200 DPM. The major pathway of endogenous GA metabolism in seedlings of the Chinese yam might be the non C-13 hydroxylation pathway.

키워드

참고문헌

  1. Bohner, J, P. Hedden, E. Bora-Haber, and F. Bangerth. 1988. Identification and quantification of gibberellins in fruits of Lycopersicon esculentum, and their relationship to fruit size in L. esculentum and L. pimpinellifolium. Physiol. Plant. 73 : 248-353
  2. Gaskin, P. and J. MacMillan. 1991. GC-MS of the gibberellins and related compounds: Methodology and a library of spectra. Cantock's Enterprises, Bristol, UK
  3. Kim, S. K., S. C. Lee, D. H. Shin, S. W. Jang, J. W. Nam, and I. J. Lee, 2003. Quantification of endogenous gibberellins in leaves and tubers of Chinese yam, Dioscorea opposita Thunb. cv. Tsukune during tuber enlargement. Plant Growth Regul. 39(2): 125-130 https://doi.org/10.1023/A:1022569506883
  4. Lee, I. J., K. R. Foster, and P. W. Morgan. 1998. Photopehod control of gibberellin levels and flowering in Sorghum. Plant Physiol. 116: 1003-101 https://doi.org/10.1104/pp.116.3.1003
  5. Okagami, N. 1972. The nature of gibberellin-induced dormancy in aerial tubers of Begonia evansiana. Plant Cell Physiol 13 : 763-771 https://doi.org/10.1093/oxfordjournals.pcp.a074789
  6. Okagami, N. and N. Tanno. 1993. Gibberellic acid-induced prolongation of the dormancy in tubers or rhizomes of several species of East Asian Dioscorea. Plant Growth Regulation 12 : 119-123 https://doi.org/10.1007/BF00144592
  7. Okagami, N. and M. Nagao. 1971. Gibberellin-induced dormancy in bulbils of Dioscorea. Planta 101 : 91-94 https://doi.org/10.1007/BF00387694
  8. Okagami, N. and N. Tanno. 1977. Dormancy in Dioscorea: generality of gibberellin induced dormancy in asexual dormant organs. Plant Cell Physol 18 : 309-316 https://doi.org/10.1093/oxfordjournals.pcp.a075436
  9. Sponsel, V. M. 1995. Gibberellin biosynthesis and metabolism. In: Davies PJ (ed.): Plant Hormones: Physiology, Biochemistry and Molecular Biology, Kluwer, Dordrecht, The Netherlands, pp. 66-97
  10. Tanno, N., T. Yokota, M. Abe, and N. Okagami. 1992. Identification of endogenous gibberellms in dormant bulbils of chinese yam, Dioscorea opposita. Plant Physiol. 100 : 1823-1826 https://doi.org/10.1104/pp.100.4.1823
  11. Zhu, Y. X. and P. J. Davies. 1991. The identification and metabolism of GAg and its metabolites in seed coats and shoots of pea, line G2. Plant Growth Regulation. 10 : 13-26 https://doi.org/10.1007/BF00035127