Determination of C3G Content in Blackish Purple Rice using HPLC and UV-Vis Spectrophotometer

  • Ryu, Su-Noh (Dept. of Agricultural Sci. Korea Nat'l Open Univ.) ;
  • Park, Sun-Zik (Dept. of Agricultural Sci. Korea Nat'l Open Univ.) ;
  • Kang, Sam-Sik (National Products Research Institute, Seoul Nat'l Univ.) ;
  • Han, Sang-Jun (National Products Research Institute, Seoul Nat'l Univ.)
  • 발행 : 2003.12.01

초록

Cyanidin 3-glucoside (C3G) content contained in the grains of blackish purple rice varieties, Heugjinjubyeo, Kilimheugmi, Heugnambyeo, Sanghaehy-anghyeolla, and the progenies derived from their crosses was evaluated by HPLC and UV-Vis spectroscopy. C3G content was higher in the range of 10-30% by using UV-Vis method compared to HPLC method. A significant linear relationship was, however, observed between two analytical methods. The correlation coefficient was 0.98. Thus, this results suggested that it would be able to use UV-Vis spectroscopy to determine C3G content which does not demanded precise value like selection.

키워드

참고문헌

  1. Cho, M. H., Y. S. Paik, H. H. Yoon, and T. R. Hahn. 1996. Chemical structure of the major color component from a korean pigmented rice variety. Agr. Chern. & Biotech. 39(4) : 304-308
  2. Choi, H. C. and S. K. Oh. 1996. Diversity and function of pigments in colored rice. Korean J. Crop Sci. 41 : 1-9
  3. Costantion, L., A. Albasini, G. Rastell, and S. Benvenuti. 1992. Activity of polyphenolic crude extracts as scavengers of superoxide radicals and inhibitors of xanthine oxidase. Planta Med., 58 : 342-344 https://doi.org/10.1055/s-2006-961481
  4. Drenska, D., I. Bantutova, and R. Ovcharov. 1989. Anticonvulsant effect of anthocyanins and antioxidants. Farmatsiya(Sojia)39 : 33
  5. Gabar, E. 1988. Possible biological role of some anthocyanins in food. Bull Liaison-Group Polyphenols. 14: 130-133
  6. Harborne, J.B. and T. J. Mabry. 1982. The anthocyanins. In; The flavonoids: Advances in research. Chapman and Hall, London. pp: 160-163
  7. Harborne, J. B. and R. J.Grayer. 1988. The anthocyanins. In; The Flavonoids: Advances in Research Since 1980; Harborne, JB., ed. Chapman and Hall, London. pp. 1-20
  8. Mazza, G. and E. Miniati. 1993. Anthocyanins in Fruits, Vegetables, and Grains CRC Press. pp : 1-23
  9. Nagai I, G. Suzushino, and Y. Tsuboki. 1960. Anthoxanthins and anthocyanins in Oryzaceae. I. Jap. J. Breed. 10(4) : 47-56
  10. Palmer R. G. and T. C. Kilen. 1987. Qualitative genetics and cytogenetics. In J.R. Wilcox (ed.) Soybean: Improvement, production, and uses. 2nd ed. Agron. Monogr. 16. ASA, CSSA, and SSSA, Madison, WI. pp. 135-209
  11. Park S. Z., J. H. Lee, S. J. Han, H. Y. Kim, and S. N. Ryu. 1998. Quantitative analysis and varietal difference of cyanidin 3-giucoside in pigmented rice. Korean J. Crop Sci. 43(3) : 179-183
  12. Reddy V. S., K. V. Goud, R. Sharma, and A. R. Reddy. 1994. Ultraviolet-B-responsive anthocyanin production in a rice cultivar in associated with a specific phase of phenylalanine ammonialyase biosynthesis. Plant Physiol. 105 : 1059-1066. https://doi.org/10.1104/pp.105.4.1059
  13. Ryu S. N., S. Z. Park, and C. T. Ho. 1998. High performance liquid chromatographic determination of anthocyanin pigments in some varieties of black rice. J. Food and Drug Analysis 6(4) : 729-736
  14. Ryu S. N., S. J. Han, S. Z. Park, and H. Y. Kim. 2000. Antioxidative activity and varietal difference of cyanidin 3-glucoside and peonidin 3-glucoside contents in pigmented rice. Korean J. Crop Sci. 45(4) : 257-260
  15. Takeoka, R. Gary, T. Dao Lan, Gerhard H. Full, Rosalind Y. Wong, Leslie A. Harden, Richard H. Edwards, and Jose De J. Berrios. 1997. Characterization of Black Bean (Phaseolus vulgaris L.). J. Agric. Food Chern. 45 : 3395-3400 https://doi.org/10.1021/jf970264d
  16. Tsuda, T., K. Ohshima, S. Kawakishi, and T.Osawa. 1994. Antioxidative pigments isolated from the seeds of Phaseolus vulgaris L. J. Agic. Food Chern. 42 : 248-251 https://doi.org/10.1021/jf00038a004
  17. Vlaskovska M., D. Drenska, and R. Ovcharov. 1990. Effect of antioxidants, alone and in combination, on the inflammatory process, Prohl. Vutr. Med. 18: 13-19