Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Bacillus sp. Producing Xylanase and Cellulase and Optimization of Medium Conditions for Its Production.

Xylanase, Cellulase의 생산성이 높은 Bacillus sp.의 분리 및 효소생산을 위한 배지조건의 최적화

  • 정원형 (건국대학교 동물자원연구센터) ;
  • 양시용 (건국대학교 생명공학과) ;
  • 송민동 (건국대학교 생명공학과) ;
  • 하종규 (서울대학교 동물자원과학과) ;
  • 김창원 (건국대학교 동물자원연구센터)
  • Published : 2003.12.01
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Abstract

A bacterium producing the extracellular xylanase and CMCase was isolated from soil and has been identified as Bacillus sp. The isolate, named Bacillus sp. A-7, was shown to be very similar to Bacillus licheniformis on the basis of its biochemical and physiological properties. The maximum xylanase and CMCase production were obtained when 2.0% (w/v) glucose and 0.3% (w/v) yeast extract were used as carbon source and nitrogen source, respectively. The best mineral conditions for xylanase and CMCase production were 0.1%(w/v) $CaC1_2$. Among the various feedstuffs, 1.0%(w/v) soybean meal was selected for the best xylanase and CMCase production.

가축 사료첨가용 효소생산을 목적으로 xylanase 및 CMCase를 동시에 분비하는 미생물을 분리한 후 가장 생산성이 높은 균주를 선발하였다. 선발된 미생물을 동정한 결과 Bacillus sp.에 속하는 균주로 Bacillus sp. A-7로 명명하였으며, B. licheniformis로 동정되었다. 효소생산을 위한 최적 액상배양조건은 탄소원으로 glucose 2.0%(w/v), 질소원으로 yeast extract 0.3%(w/v), 미네랄은 CaCl$_2$ 0.1% (w/v)인 것으로 나타났다. 저렴한 효소생산을 위한 배지조성을 얻기 위해 가축사료로 이용되는 저가의 원료사료를 이용하여 효소생산성을 파악한 결과 대두박 1.0%(w/v)와 당밀 0.5%(w/v)가 특히 xylanase 생산성이 높은 것으로 나타났다. 특히, 대두박 1.0%(w/v)를 기본배지로 하여 ammonium nitrate 0.3%(w/v) 및$ CaCl_2$ 0.1% 첨가가 적합한 배지조성으로 나타났다.

Keywords

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