Abstract
Coriolus versicolor was grown in a defined synthetic liquid medium and citrus extracts, and the culture extracts were examined for antioxidant activity, nitrite scavenging activity, and in vitro anticancer activity against HeLa, PC-3, HepG2, and A-549 cells. Whereas the culture extracts obtained from the synthetic medium and the un-inoculated citrus extract showed 60 and 22％ of the 1,1-diphenyl-2-picrylhydrazyl radical scavenger activity, the culture extracts obtained from the citrus extracts medium exhibited antioxidant activity up to 89％. The nitrite scavenging activity of the culture extracts obtained from the citrus extracts medium and the synthetic liquid medium, and the un-inoculated citrus extract at pH 1.2 were up to 67, 55, and 34％, respectively. The culture extract obtained from the synthetic liquid medium inhibited the growth of HeLa, PC-3, HepG2, and A-549 up to 66, 23, 18, 10％ at 48 h of incubation, respectively; however, the culture extract obtained from the citrus extracts medium inhibited the growth of HeLa, PC-3, HepG2, and A-549 up to 75, 82, 55, and 82％, respectively. As a negative control, the un-inoculated citrus extract was examined in the same way and inhibited the growth of HeLa, PC-3, and HepG2 cells 20, 6, and 15％ at 48 h incubation, respectively; the inhibition of A-549 cell growth was negligible. These results clearly showed that the fermentation of C. versicolor in the citrus extracts rather than in the defined synthetic medium significantly enhanced the anticancer activity, antioxidant activity, and nitrite scavenging activity.
운지버섯을 희석 감귤농축액과 일반 합성배지에서 각각 배양한 배양물을 열탕추출하고 여과하여 얻어진 버섯 배양추출물과 버섯을 접종하지 않은 희석 감귤농축액 자체에 대한 항산화 활성, 아질산 제거능, 그리고 항암활성을 조사 비교하였다. DPPH 전자공여를 이용한 항산화 활성에서 희석 감귤농축액에서 얻어진 배양추출물(Extract-I)은 89%, 일반 합성배지에서 얻어진 배양추출물(Extract-II)은 66%, 접종하지 않은 희석 감귤농축액 자체(Extract-III)는 22%의 항산화 활성을 나타내었다. 아질산 제거능은 배양추출물 모두 산성 조건일수록 증가하였으며, Extrac-I은 67%, Extract-II는 54%, 그리고 Extract-III는 34%의 아질산 제거능을 보였다. HeLa, PC-3, HepG2 및 A-549 세포에 대한 항암활성을 조사한 결과, Exract-I는 각각의 암세포들에 대해서 순차적으로 75%, 82%, 55%, 그리고 82%의 높은 생육 저해 활성을 보였다. 반면에 Extract-II는 HeLa cell에 대해서만 66%의 생육저해를 보였고, Exoact-III는 모든 암세포의 생육을 저해하지 않는 것으로 조사되었다. 따라서 운지버섯을 희석 감귤농축액에 배양함으로서 일반 합성배지에서 배양하거나 감귤농축액 자체 보다 항산화 활성과 항암활성을 현저히 증가시킬 수 있었다.
