Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimization for Novel Glucanhydrolase Production of Lipomyces starkeyi KSM 22 by Statistical Design

  • PARK, JUN-SEONG (Department of Material Engineering and Biochemical Engineering, Chonnam National University) ;
  • BYUNG-HOON KIM (Engineering Research Institute, Chonnam National University) ;
  • JIN-HA LEE (Engineering Research Institute, Chonnam National University) ;
  • EUN-SEONG SEO (Department of Material Engineering and Biochemical Engineering, Chonnam National University) ;
  • KAB-SU CHO (Department of Material Engineering and Biochemical Engineering, Chonnam National University) ;
  • HYUN-JUNG PARK (Department of Material Engineering and Biochemical Engineering, Chonnam National University) ;
  • HEE-KYOUNG KANG (Engineering Research Institute, Chonnam National University) ;
  • SUN-KYUN YOO (Department of Food and Biotechnology, Joong Bu University) ;
  • MYUNG-SUK HA (Engineering Research Institute, Chonnam National University)
  • Published : 2003.12.01
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Abstract

Response surface methodology was applied to find the optimum conditions for the production of DXAMase (containing both dextranase and amylase activities) based on the cultivation variables (pH, temperature, and agitation rate). The experimental values from the model equation conceded with predicted values in which the predicted values for dextranase and amylase activities were 2.26 and 3.52 U/ml at pH 4, $28^{\circ}C$, 235 rpm, and the corresponding experimental values were 2.41 and 3.68 U/ml, respectively.

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References

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