Journal of Microbiology and Biotechnology

  • 제13권6호
  • /
  • Pages.846-852
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  • 2003
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Differentiation of Actinomycete Genera Based on Partial rpoB Gene Sequences

  • Kim, Bum-Joon (Department of Microbiology, College of Medicine, Seoul National University) ;
  • Koh, Young-Hwan (Department of Food Science and Engineering, Cheju National University) ;
  • Chun, Jong-Sik (School of Biological Sciences, Seoul National University) ;
  • Kim, Chang-Jin (Department of Microbiology, College of Medicine, Konkuk University) ;
  • Lee, Seung-Hyun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Moon-Jae (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Hyun, Jin-Won (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Lee, Keun-Hwa (Department of Microbiology, College of Medicine, Seoul National University) ;
  • Cha, Chang-Yong (Department of Microbiology, College of Medicine, Seoul National University) ;
  • Kook, Yoon-Hoh (Department of Microbiology, College of Medicine, Seoul National University)
  • 발행 : 2003.12.01
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초록

rpoB DNAs (279 bp) from 34 species of 5 actinomycete genera were sequenced and a phylogenetic tree was constructed based on the sequences obtained. The genera were clearly differentiated in the rpoB tree, forming clades specific to their respective genus. In addition, 2 signature amino acid residues specific to Streptomyces were found in a multiple alignment of the deduced amino acid sequences. To empirically confirm that this rpoB gene analysis system could be used to differentiate actinomycete isolates, the proposed system was used to identify 16 actinomycete isolates from Jeju Island. All isolates were successfully differentiated into the genera Streptomyces and Micromonospora. Accordingly, this is the first report that an rpoB sequence analysis has been effectively used to differentiate actinomycete strains at the genus level.

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참고문헌

  1. Identification of Streptomyces sp. producing new polyene antibiotics and in vivo antimicrobial activity of tetrin C against phytopathogenic fungi v.12 Choi,W.C.;S.Y.Hwang;T.K.Park;S.K.Kim
  2. Extremophiles v.2 Taxonomy and biotransformation activities of some deep-sea actinomycetes Colquhoun,J.A.;S.C.Heald;L.Li;J.Tamaoka;C.Kato;K.Horikoshi;A.T.Bull https://doi.org/10.1007/s007920050069
  3. Gene v.115 Numerical classification and identification of Streptomyces species - a review Goodfellow,M.;E.V.Ferguson;J.J.Sanglier https://doi.org/10.1016/0378-1119(92)90563-5
  4. Int. J. Syst. Evol. Microbiol. v.50 Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness Kasai,H.;T.Tamura;s.harayama https://doi.org/10.1099/00207713-50-1-127
  5. FEMS. Microbiol. LEtt. v.151 Application of the variable region in 16S rDNA to create an index for rapid species indentification in the genus Streptomyces Kataoka,M.;K.Ueda;T.Kudo;T.Seki;T.Yoshida https://doi.org/10.1111/j.1574-6968.1997.tb12578.x
  6. J. Microbiol. Biotechnol. v.12 Antifungal activities against Plasmodiophora brassicae causing club root Kim,B.J.;G.J.Choi;K.Y.Cho;H.Yang;C.Shin;C.H.Lee;Y.Lim
  7. J. Microbiol. Biotechnol. v.11 Streptomyces showing antifungal activities against six plant pathogenic fungi Kim,B.J.;M.Cho;J.C.Kim;K.Y.Cho;G.J.Choi;C.H.Lee;Y.Lim
  8. J. Clin. Microbiol. v.37 Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB) Kim,B.J.;S.H.Lee;M.A.Lyu;S.J.Kim;G.H.Bai;G.T.Chae;E.C.Kim;C.Y.Cha;Y.H.Kook
  9. J. Clin. Microbiol. v.40 Application of RNA polymerase beta0subunit gene (rpoB) sequences for the molecular differentiation of Legionella species Ko,K.S.;H.K.Lee;M.Y.Park;K.H.Lee;Y.J.Yun;S.Y.Woo;H.Miyamoto;Y.K.Kook https://doi.org/10.1128/JCM.40.7.2653-2658.2002
  10. Int. J. Syst. Bacteriol. v.46 16S ribosomal DNA analysis of the genera Micromonospora, Actinoplanes, Catellatospora, Catenuloplanes, Couchioplanes, Dactylosporangium, and Pilimelia and emendation of the family Micromonosporaceae Koch,C.;R.M.Kroppenstedt;F.A.Rainey;E.Stackebrandt https://doi.org/10.1099/00207713-46-3-765
  11. Kor. J. Appl. Microbiol. Biotechnol. v.24 Distribution pattern of soil actinomycetes at Cheju islands Kwon,O.S.;D.J.Park;C.Y.Lee;C.J.Kim
  12. J. Gen. Microbiol. v.135 New probability matrices for identification of Streptomyces Langhan,C.D.;S.T.Williams;P.H.Sneath;A.M.Mortimer
  13. Antonie Van Leeuwenhoek v.79 Rare genera of actinomycetes as potential producers of new antibioties Lazzarini,a.;L.Cavaletti;G.Toppo;F.Marinelli
  14. J. Microbiol. Biotechnol. v.12 Streptomyces with antifungal activitiy against rice blast causing rungus, Magnaporthe grisea Lee,C.H.;B.J.Kim;G.J.Choi;K.Y.Cho;H.Yang;C.Shin;S.Min;Y.Lim
  15. Can. J. Microbiol. v.48 Diversity of antifungal actinomycetes in various vegetative soils of Korea Lee,J.Y.;B.K.Hwang https://doi.org/10.1139/w02-025
  16. J. Clin. Microbiol. v.38 Differentiation of Borrelia burgdorferi sensu lato on the basis of RNA polymerase gene (rpoB) sequences Lee,S.H.;B.J.Kim;J.H.Kim;K.H.Park;S.J.Yeo;S.J.Kim;Y.K.Kook
  17. Microbiology v.141 Nucleotide sequences of streptomycete 16S ribosomal DNA: Towards a specific identification system for Streptomycetes using PCR Mehling,A.;U.F.Wehmeier;W.Piepersberg https://doi.org/10.1099/13500872-141-9-2139
  18. Int. J. Syst. Bacteriol. v.45 A taxonomic study of teh genus Streptomyces by analysis of ribosomal protein AT-L30 Ochi,K. https://doi.org/10.1099/00207713-45-3-507
  19. J. Clin. Microbiol. v.36 16S rRNA sequence diversity in Mycobacterium celatum strains caused by presence of two different copies of 16S rRNA gene Reischl,U.;K.Feldmann;L.Naumann;B.J.Gaugler;B.Ninet;B.Hirschel;S.Emler
  20. J. Microbiol. Biotechnol. v.11 Identification of Streptomyces sp. AMLK-335 producing antibiotic substance inhibitory to vancomycin-resistant enterococci Rhee,K.H.;K.H.Choi;C.J.Kim;C.H.Kim
  21. Int. J. Syst. Bacteriol. v.40 Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium Rogall,T.;J.Wolters;T.Flohr;E.C.Bottger https://doi.org/10.1099/00207713-40-4-323
  22. J. Bacteriol. v.181 Two distinct mechanisms cause heterogeneity of 16S rRNA Ueda,K.;T.Seki;T.Kudo;T.Yoshida;M.Kataolka
  23. J. Gen. Microbiol. v.129 Numberical classification of Streptomyces and related genera Williams,S.T.;M.Goodfellow;G.alderson;E.M.Wellington;P.H.Sneath;M.J.Sackin