Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Isolation and Characterization of Nucleoside Diphosphate Kinase 1 of Codonopsis lanceolata

더덕에서 Nucleoside Diphosphate Kinase 1 분리 및 분석

  • 김종학 (㈜바이오피아) ;
  • 양덕춘 (경희대학교 생명과학대학 및 한방재료가공센타)
  • Published : 2003.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The NDK1 is an ubiquitous enzyme that transfer phosphate groups from triphosphate nucleoside diphosphates(NDPs) in eukaryotes and prokaryotes. We isolated and characterized a cDNA encoding a nucleoside diphosphate kinase 1(CNDK 1) in Codonopsis lanceolata. The CNDK 1 is 444bp long and open reading frame of 447bp with a deduced amino acid of 148 residue. The CNDK 1 has an ATP binding site in 12­16 residue and phosphohistidine intermediate in 115 residue of amino acid sequence. Although several NDK 1 genes have been cloned in plants, but little is known about the functional significance of this enzyme during plant growth and development. The CNDK 1 shows the identities to Arabidopsis thaliana (71％), Oryza sativa(75％), Glycine max (79％), Brassica rapa (77％), Mesembryanthemum crystallinum (85 ％), Spinacia oleracea (83％), Pisum sativum (82％). The CNDK 1 of C. laceolata have a closer relationship of Glycine max and Pisum sativum at the phylogenic analysis.

더덕의 재배는 수익성이 높고 재배면적도 증가하지만 수요를 만족시킬 만큼 공급이 따르지 못하고 있다. 또한 재 배 상의 어려운점은 병충해, 기계수확에 의한 대규모 재배를 더욱더 곤란하게 하고 있는 실정이다. 이러한 문제점 및 환경적 스트레스에 저항하여 자랄 수 있는 식물체를 얻기 위해 더덕의 cDNA를 분석하여 스트레스 관한 유전자 Nucleoside diphosphates kinase 1(NDK 1)을 분리하여 분석하여 148개의 아미노산 서열과 다른 식물체들의 NDK 1과 높은 유사성을 가진다는 것을 알았고, 더덕의 각 조직에서 나타나는 ClNDK1의 발현을 알아보기 위해 캘러스, 잎, 줄기, 뿌리 조직의 전체 RNA를 추출하여 cDNA를 합성하고 PCR을 수행하였다 RT­PCR 분석 결과, ClNDK1은 조직 특이성 없이 캘러스, 잎, 줄기, 뿌리 조직에 대해서 모두 발현이 되었으며, 발현량, 역시 큰 차이 없이 모든 조직에서 동일하게 발현되었다. NDKs 는 환경 스트레스에 저항성을 가진다고 알려져 있지만 NDK 1 대한 연구는 아직까지 부족한 상태이다. 우리는 더덕에서 분리한 ClNDK1의 스트레스 저항성에 대해서 지속적으로 연구를 수행 할 것이다.

Keywords

References

  1. J Mol Biol v.215 Basic local alignment search tool Altschul,S.F.;W.Gish;W.Miller;E.W.Myers;D.J.Lipman https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Nature v.401 Pytochrome signalling is mediated through mucleoside diphosphate kinase 2 Choi,G.;H.Yi;J.Lee;Y.K.Kwon;M.S.Soh;B.Shin;Z.Luka;T.R.Hahn;P.S.Song https://doi.org/10.1038/44176
  3. Kor. J. Phamacog v.8 Studies on the terpenoid component of the rootsl of Codonopsis laceolata Bent. et Hook Chung,B.S.;D.S.Na
  4. Int J Cancer v.73 Dawn-regulation of the nm23.h1 gene inhibits cell proliferation Cipollini,G.;a.Berti;L.Fiore;G.Raimaldi;F.Basolo;G.Merlo;G.Bevilacqua;M.Caligo https://doi.org/10.1002/(SICI)1097-0215(19971009)73:2<297::AID-IJC22>3.0.CO;2-B
  5. Eur J Biochem v.234 A novel serine/threonine specific phospho- transferase activity of Nm23/nucleoside diphosphate kinase Engel,M.;M.Veron;B.Theisinger;M.L.Lacombe;T.Seiv;S.Dooley;C.Welter https://doi.org/10.1111/j.1432-1033.1995.200_c.x
  6. Plant Mol Biol v.251 Molecula cloning, sequence determination and heterozygous expression of nucleoside diphosphates kinase of Pisum sativum Finan,P.M.;I.White;S.Redpath;J.Findlay;P.Miller
  7. J. Biol. Chem. v.266 Nucleoside diphosphate kinase from human erythrocytes: structrual characterization of the two polypeptide chanin responsible for heterogenety of the hexameric enzyme Gilles,A.M.;E.Prescan;A.Vonica;I.Lascu
  8. Plant Mol Biol v.25 Isolation of mRNA encoding a necleside diphosphate kinase from tomato that is up-regulated by wounding Harris,N.;J.Taylor;J.Roberts https://doi.org/10.1007/BF00029611
  9. J Korean Agricultural Chemical Society v.27 Chemical compositions of dried wild and cultivated Codonopsis lanceolata Lee,S.K.
  10. A pictorial book of the Korean flora Lee,C.B.
  11. Plant Physiology v.122 Nucleoside diphosphates kinase required for coleoptile elongation in rice Ling,P.;K.Maki;Y.Akira;U.Hirofumi https://doi.org/10.1104/pp.122.2.447
  12. Planta v.1964 Nucleoside diphosphate kinase from pea chloroplasts: purification, cDNA cloning and import into chloroplasts Lubeck,J.;J.Soll
  13. Plant physiology v.126 Heat stress response in pea involves interaction of mitochondrial nucleoside diphosphate kinase with a novel 86-kilodalton protein Martha,L.;G.Escobar;M.Salla;H.Gunilla;F.Jens;K.Carina https://doi.org/10.1104/pp.126.1.69
  14. Journal of Experimental Botany v.53 Isolation of a cDNA for a nucleoside diphosphte kinase capable of phosphorylating the kinase domain of the selfincompatibility factor SRK of Brassica campestris Matsushita,Y.;T.Suzuki;R.Kubota;M.Mori;H.Shimosata;M.Watanave;T.Kayano;T.Nishio;H.Nyunoya https://doi.org/10.1093/jexbot/53.369.765
  15. J. Biol Chem. v.268 A serine phophorylation of Nm23, and not its nucleosode diphosphate kinase activity, correlates with suppression of tumor metastatic potential McDonald,N.J.;De la Rosa A;M.Benedict;J.Freije;H.Klutsch;P.Steeg
  16. Plant Cell Rep v.10 High frequency sometic embryogenesis and plant regeneration in tissue cultures of Codonopsis lanceolata Min,S.R.;S.Yang;J.Liu;P.Choi;W.Soh
  17. PNAS v.100 NDP kinase 2 interacts with two oxidative stress-activate MARKs to regulate cellular redox state and enhances multiple stress tolerance in trangenic plants Moon,H.J.;B.Y.Lee;G.Choi;C.Lim;D.Yun https://doi.org/10.1073/pnas.252641899
  18. Eur J Biochem v.190 Osmotic stress and abscisic acid regulate the expression of the Em gene of wheat Morris,P.C.;A.Kumar;D.Bowles;A.Cuming https://doi.org/10.1111/j.1432-1033.1990.tb15618.x
  19. Plant Physiol v.113 Immunological detection of potential signal-transduction proteins expressed during wheat somatic tissue culture Nato.A.;A.Mirshahi;G.Tichtinsky;M.Mirshahi;J.Faure;D.Lavergne;De Buyser J.;C.Jena;G.Ducreux;Y.Henry https://doi.org/10.1104/pp.113.3.801
  20. Planta v.208 The effect of ethylene and cytokinin on guanosine 5'-triphosphate binding and protein phosphorylation in leaves of arabidopsis thaliana Nevikava,G.V.:I.Moshkov;A.Smith;O.Kulaeva;M.Hall https://doi.org/10.1007/s004250050555
  21. Arch Bioshem Biophys v.15 The amino acid sequence of nucleoside diphosphates kinase 1 from spinach leaves, as deduced from the cDNA sequence Nomera,T.;K.Yatsunami;A.Honda;Y.Sugimoto;T.Fukui;J.Zhang;J.Yamamoto;A.Ichikawa
  22. Biochem Biophys. Acta v.1449 Wild-type NM23-H1, but not its S120 mutants, suppresses desensitization of muscarinic potassiun current Otero,A.;M.B.Doyle;M.T.Hartsong;P.S.Steeg https://doi.org/10.1016/S0167-4889(99)00009-9
  23. the Enzyme v.8 Nucleoside diphosphates kinase Park,P.E.;R.P.Agarwal;P.D.Boyer(ed.) https://doi.org/10.1016/S1874-6047(08)60069-4
  24. Korean J. Plant Tissue Culture v.27 Introduction of RAG25 gene into Codonpsis lanceolata by Agrobacterium tumefaciens Shin,J.H.;M.C.Park
  25. J. oriental Bot. Ros v.41 Screenging test for antitumor activity of Eragrostis ferruginea Thumb Sin,S.C.;J.L.Lee;E.S.Lee;E.S.Yoon
  26. Biochim Biophys Acta v.21 A plant nucleoside disphosphates kinase homologous to the human Nm 23 gene product: pruification and characterization Sommer,D.;P.S.Song
  27. J Photochem Photobiol B. v.452 Phytochrome-mediated light signals are transduced to triphosphate nucleoside diphosphates in Pisum sativum L. cv. Alaska. Tanaka,N.;T.Ogura;T.Noguchi;H.Hirano;N.Yabe;K.Hasunuma
  28. J Biol Chem v.274 UV-resopnsive genes of Arabidopsis revealed by similarity to the Gcn4-mediated UV response in yeast Zimmermann,S.;A.Baumann;K.Jaekel;I.Marbach;D.Engelberg;H.Frohnmeyer https://doi.org/10.1074/jbc.274.24.17017