The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Antimutagenic and Antioxidative Effects of Methanol Extract of Pine Pollen

송화 메탄올 추출물의 항산화적 항돌연변이 효과

  • Published : 2003.12.01
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Abstract

This study was performed to investigate the antimutagenic and antioxidative activities of pine pollen with respect to the microbial mutation induced by various mutagens such as 1-NP, daunomycin, 2-NF, MNNG, NaN$_3$, 4NQO, 4-NOPD, AFB$_1$, Trp-P-1, 2-AF and oxidative mutagens such as t-BOOH, H$_2$O$_2$. Pine pollen, originally extracted with hexane, was reextracted with 70% methanol. The results obtained using the methanol extract, in terms of the antimutagenicity observed in relation to ten kinds of mutagens, showed that it exhibited 17.8, 82.2 and 80.9% inhibitory effects against daunomycin, AFB$_1$, and Trp-P-1, respectively, in Salmonella. typhimurium TA98 and a 72.3% inhibitory effect against AFB$_1$in S. tyPhimurium TA100. In terms of the antimutagenicity exhibited in relation to t-BOOH, a 72.3% inhibitory effect was observed, but no antimutagenicity was observed in relation to the other mutagens and strains. The methanol extract was further fractionated by chloroform, ethyl acetate, n-butanol. In S. typhimurium TA98, the chloroform(150 $\mu\textrm{g}$/plate) fraction showed strong antimutagenic effects of 55.6%, 93.7% and 93.5%, while the ethyl acetate(100 $\mu\textrm{g}$/plate) fraction showed 11.4%, 74.3% and 85.2% in relation to the mutagenicity induced by daunomycin, AFB$_1$and Trp-P-1, respectively. In S. typhimurium TA100, the chloroform and ethyl acetate fractions showed antimutagenic effects of 95.1% and 62.5%, respectively, on the mutagenicity induced by AFB$_1$. In S. typhimurium TA102, the chloroform fraction showed an antimutagenic effect of 93.6% on the mutagenicity induced by t-BOOH.

직ㆍ간법 변이원과 산화적 돌연변이원에 대한 송화분 추출물의 항돌연변이원성에 대하여 Ames test로 조사하였다. 메탄을 추출물을 이용하여, 10종의 돌연변이에 대한 돌연변이 억제효과를 검색한 결과 S. typhimurium TA98에서는 daunomycin, AFB$_1$과 Trp-P-1의 변이원성에 대해서만 각각 17.8, 82.2, 80.9%의 돌연변이 억제효과를, S. typhimurium TA100에서는 AFB$_1$의 변이원성에 대해서만 72.3%의 돌연변이 억제효과를 나타내었으며, S. typhimurium TA102, 104에서 t-과산화부틸과 $H_2O$$_2$의 돌연변이원성에 대한 메탄을 추출물의 돌연변이 억제 효과는 t-과산화부틸을 돌연변이원으로 한 S. typhimurium TA102에서 16.3%의 억제효과를 선택적으로 나타내었다. 따라서 메탄을 추출물을 클로르포름, 에틸아세테이트, 부탄올, 물 순으로 순차용매 분리한 다음, 이 분획물에 대한 항돌연변이원성을 검색하였다. 그 결과 S. typhimurium TA98에서 daunomycin, AFB$_1$과 Trp-P-1에 대해서 플로로포름 분획물은 각각 55.6, 93.7, 93.5%, 에틸아세테이트 분획물은 각각 11.4, 74.3, 85.2%, 부탄을 분획물에서는 10.9, -5.6, 6.8%, 물 분획물은 각각 -3.4, -3.8, 4.6%의 돌연변이 억제효과를 나타내었다. S. typhimurium TA100에서는 AFB$_1$에 대해 클로로포름 분획물과 에틸아세테이트 분획물이 각각 95.1, 62.5%의 억제효과를 나타내었으며, S. typhimurium TA102에서는 t-과산화부틸에 의해 유도된 산화적 돌연변이에 대해 클로로포름 분획물이 93.6%로 매우 강한 억제효과를 나타내었으며, 기타 분획물에서는 매우 낮은 억제효과를 나타내었다.

Keywords

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