Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Green Tea Maintains Antioxidative Defense Enzyme Activities and Protects Against Lipid Peroxidation in Rat Gastrocnemius Muscles After Aerobic Exercise

  • Chai, Young-Mi (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Rhee, Soon-Jae (Department of Food Science and Nutrition, Catholic University of Daegu)
  • Published : 2003.12.01
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Abstract

This study investigated the effects of green tea on the muscle antioxidative defense system in the white & red gastrocnemius muscles of rats after aerobic exercise. Male Sprague-Dawley rats weighing 150 10 g were randomly assigned to a control group, non-exercise with green tea group (G group), and exercise training group. The exercise training group was then further classified as the training (T) group and training with green tea (TG) group, the latter of which was supplemented with green tea in the drinking water during the experimental period. The rats in the exercise training groups (T and TG) were subjected to aerobic exercise on a treadmill 30 min/day at a speed of 28 m/min (7% incline) 5 days/week, while the other groups (control and G group) were cage confined for 4 weeks. Thereafter, the rats were sacrificed with an injected overdose of pentobarbital just after running. In the white muscle, the xanthine oxidase (XOD) activities were 71 % higher in the T group compared to control group, whereas the TG group had the same activity as the control group. The XOD activities in the red gastrocnemius muscle exhibited the same tendency as in the white muscle. The superoxide dismutase (SOD) activity in the white muscle was lower in the T group compared with the control group, yet significantly higher in the TG group compared with the T group. The SOD activities in the red gastrocnemius muscle exhibited the same tendency as in the white gastrocnemius muscle. The glutathione peroxidase (GSHpx) activities in the white & red gastrocnemius muscles were 43 % lower in the T group compared with the control group, yet the activities in the TG group remained at control levels. The glutathione S-transferase (GST) activity in the white muscle was not significantly different among any of the three groups, but in the red gastrocnemius muscle, the TG group had the same activity as in the control group. The thiobarbituric acid reactive substance (TBARS) contents in the white & red gastrocnemius muscles were higher in the T group than in the control but the control and TG groups had the same concentrations of TBARS. In conclusion, the supplementation of green tea in rats subjected to aerobic exercise was found to reduce the peroxidation of muscle lipids by enhancing the antioxidative defense mechanism.

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References

  1. Kor J Nutr v.29 Nutritional problems in Korean pattern of disease incidence and nutrition in Korea Lee HG
  2. Med Sci Sports Exerc v.26 Physical activity, diet and health independent and interactive effects Wood PD
  3. Biochem Biophys Res Communi v.107 Free radicals and tissue damage produced by exercise Davies KJ;Quintanilha AT;Brooks GA;Packer L https://doi.org/10.1016/S0006-291X(82)80124-1
  4. Int Sports Med v.12 Influence of aging training and acute physical exercise on plasma glutathione and lipid peroxides in man Kretzhchmer M;Muller D;Hubscher J:MarinE;Klinger W https://doi.org/10.1055/s-2007-1024671
  5. Inflammation v.815 NADPH-oxidase activity of stimulated-neutrophils is markedly in creased by serum Anderson R;Theron AJ;Steenkamp KJ
  6. Mol Cell Biochem v.111 Dietary supplementation of vitamin E protects heart tissue exercise induced oxidant stress Kumar CT;Reddy VK;Prasad M;Thyagaraju K;Reddanna P
  7. J Appl Physiol v.60 Vitamin E deficiency and vitamin C supplements exercise and mitochondrial oxidation Gohil K;Packer L;Lumen B;Brooks GA
  8. Biochem Biophys Res Commun v.189 Vitamin E inhibits protein oxidation in skeletal muscle of resting and exercised rats Reznick AZ;Witt E;Matsumoto M;Packer L
  9. J Appl Physio v.73 Reactive oxygen in skeletal muscle. Intracellular oxidant kinetics and fatigue in vitro Reid MB;Haack KE;Frankchek KM;Valberg PA;Kobzik L;West MS
  10. Korean J Nutrition v.35 Effect of glucuronic acid derivatives isolated from xylan on antioxidative defense system in rat white gastrocnemius after aerobic exercise Kim KR;Rhee SJ
  11. J Appl Physiol v.92 Effects of exercise training and antioxidant R-ALA on glucose transport in insulin-sensitive rat skeletal muscle Saengsirisuwan V;Perez FR:Kinnick TR;Henriksen EJ
  12. Korean J Nutrition v.34 Effects of glucuronic acid derivatives isolated from xylan on antioxidative defense system and muscle fatigue recovery after aerobic exercise Choi HM;Lee SC;Ryu SP;Rhee IK;Joo GJ;Rhee SJ
  13. Med Sci Sports Exerc v.32 Vitamin E regulates changes in tissue antioxidants induced by fish oil and acute exercise Atalay M;Laaksonen DE;Khanna S;Kaliste-Korhonen E;Hanninen O;Sen CK https://doi.org/10.1097/00005768-200003000-00008
  14. J Appl Physiol v.86 Alpha-lipoic acid supplementation tissue glutatione homeostasis at rest and after exercise Khann S;Atalay M;Laaksonen DE;Gul M;Roy S;Sen CK https://doi.org/10.1063/1.370869
  15. Free Radic Biol v.21 Effects of exhaustive exercise and vitamin $B_6$ deficiency on free radical oxidative process in male trained rats Benderitter M;Hadj-Saad F;Lhuissier M;Maupoil V;Guilland JC;Rochett L https://doi.org/10.1016/0891-5849(96)00047-0
  16. J Appl Physiol v.91 Interactions of exercise training and lipoic acid on skeletal muscle glucose transport in obese Zucker rat Saengsirisuwan V;Kinnick TR;Schmit MB;Hentriksen EJ
  17. Biochim Biophys Acta v.1127 Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats Ikeda I;Imasato Y;Sasaki E;Nagao H;Tekeo F;Sugano M https://doi.org/10.1016/0005-2760(92)90269-2
  18. Argic Biol Chem v.53 Antibacterial substances in Japanese green tea extract against Streptococcus mutans. a carcinogenic bacterium Sakanaka S;Kim M;Taniguchi M;Yamamoto T https://doi.org/10.1271/bbb1961.53.2307
  19. J Natl Cancer Inst v.85 Tea and cancer Yang CS;Wang ZY https://doi.org/10.1093/jnci/85.13.1038
  20. Nippon Nogeikagaku Kaish v.59 Antioxidative activity of tea leaf catechins Matsuaki T;Hata Y https://doi.org/10.1271/nogeikagaku1924.59.129
  21. Acta Biol Exp Sinica v.19 A preliminary study on the antimutagenicity of green tea antioxidants Cheng SJ;Ho CT;Lou HZ;Bao VD;Jian YZ;Li MH;Gao YN;Zhu GF;Bai JF;GIo SP;LI XQ
  22. J Kor Soc Food Sci Nutr v.31 Effects of green tea on hepatic antioxidative defense system and muscle fatigue recovery in rat after aerobic exercise Kim MJ;Rhee SJ https://doi.org/10.3746/jkfn.2002.31.6.1058
  23. J Bio Chem v.244 The regulation of liver xanthine oxidase Stripe F;Della Corte E
  24. Eur J Biochem v.47 Involvement of the superoxide anion radical in antioxidation of pyrogallol and a convenient assay for superoxide dismutase Marklund S;Mrklund G
  25. Biochem Biophys Res Commun v.71 Glutathione peroxidase activity in selenium deficiency rat liver Lawrence RA;Burk RF https://doi.org/10.1016/0006-291X(76)90747-6
  26. J Biol Chem v.249 Glutathione Stransferases. The first enzymatic step in mercapturic acid formation Habig WH;Pabst MJ;Jakoby WB
  27. Clin Chim Acta v.90 Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method Satoh K https://doi.org/10.1016/0009-8981(78)90081-5
  28. J Biol Chem v.193 Protein measurement with the folin phenol reagent Lowry OH;Rosebrough NJ;Farr AL;Randall RJ
  29. Principles and procedures of statistics Sreel RGD;Torrie JH
  30. Biochem J v.131 Characterization of alternative molecular forms of xanthine oxidase in the mouse Duke EJ;Joyce P;Ryan JP
  31. J Appl Physiol v.68 Skeletal muscle oxidative capacity antioxidant enzymes and exercise training Laughlin MH;Simpson T;Sexton WL;Brown OR;Smith JK;Korthuis RJ
  32. Physiol Rev v.59 Hydroperoxide metabolism in mammalian organs Chance B;Sies H;Boveris A
  33. PhD Dissertation. Korea Univeristy The effect of antioxidants ingestion on the antioxidant enzymes and immune function in endurance training Oh BS
  34. Alcol Clin Exp Res v.12 Depletion of hepatic glutathione by ethanol occurs independently of ethanol metabolism Speisky H;Kera Y;Penttla KE;Israel Y;Lindros KO
  35. Kor J Gerontol v.8 Changes of the antioxidant system and tissue damage on kidney of streptozotocin-induced diabetic rats Rhee SJ
  36. J Appl Physiol v.83 Fish oil and vitamin E supplementaion in oxidative stress at rest and after physical exercise Sen CK;Atalay M;Agren J;Laaksonen DE;Roy S;Hanninen O

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