Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Identification of a Marine Bacterium, Pseudomonas sp. BK1 Producing Extracellular Enzymes Capable of Decomposing Multiple Complex Polysaccharides

복합 다당류 분해 효소들을 생산하는 해양미생물 Pseudomonas sp. BK1의 분리 및 특성

  • Kim, Beom-Kyu (Biohub Co. Ltd., Institute of Life Science Gyeongsang National University) ;
  • Jeon, Beong-Sam (Biohub Co. Ltd., Institute of Life Science Gyeongsang National University) ;
  • Cha, Jae-Young (Biohub Co. Ltd., Institute of Life Science Gyeongsang National University) ;
  • Park, Jeong-Won (Biohub Co. Ltd., Institute of Life Science Gyeongsang National University) ;
  • Kim, Sam-Woong (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Ji-Yoon (Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Park, Yong-Lark (Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Cho, Young-Su (Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Song, Jae-Young (Department of Microbiology, College of Medicine, Gyeongsang National University)
  • Published : 2003.12.01
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Abstract

A marine bacterium (strain BKl) that produces extracellular enzymes capable of decomposing complex polysac-charides, such as agar, chitin, carboxymethylcellulose, xylan and mannan, was isolated from the marine red alga Porphyra dentata. Strain BKl was gram-negative, aerobic, catalase- and oxidase-positive, polarly flagellated bacilli that produce gelatinase and urease, but not decarboxylases. The G+C content of the DNA was 51.6 mol%. The major isoprenoid quinone component was identified as an ubiquinone-8, and the major cellular fatty acids were C16:0, C16:1 w6c and C18:1 w7c. Comparative 16S rRNA sequence analysis placed strain BK1 with members of the genus Pseudomonas. On the basis of phenotypic and genotypic data, the strain BK1 was shown to be a member of the subgroup of Pseudomonas, and named as Pseudomonas sp. BK1.

한천, 키틴, 셀룰로오스, 자일란, 만난과 같은 복합다당류들에 대한 분해능을 나타내는 효소들을 생산하는 해양미생물을 홍조류인 Porphyra dentata로부터 분리하였다. 분리균 BK1은 그람음성, 호기성 간균으로 DNA의 G+C함량은 51.6 mol%를 나타내었다. 주요 isoprenoid quinine 구성 성분은 ubiquinone-8로 확인되었고, 주요 세포 지방산은 C16:0, C16:1 w6c, C18:1 w7c로 밝혀졌다. 16S rRNA서열의 비교분석 결과는 분리균 BK1이 Pseudomonas 속의 일원인 것으로 확인되었다. 이러한 결과를 바탕으로 분리균 BKl은 Pseudomonas sp. BKl으로 명명하였다.

Keywords

References

  1. Microbiol. Rev. v.45 Distribution of isoprenoid quinine structural types in bacteria and their taxonomic implications Collins,M.D.;D.Jones
  2. Trends Biotechnol. v.15 Marine microbial diversity: the tip of the iceberg DeLong,E.F. https://doi.org/10.1016/S0167-7799(97)01044-5
  3. Nature v.192 Variant in the structural polysaccharides of algal cell wall Frei,E.;R.D.Preston https://doi.org/10.1038/192939a0
  4. Cur. Microbiol. v.39 Sequencing and expression of a b-mannanase gene from the extreme thermophile Dictyoglomus thermophilum Rt46B.1, and characteristics of the recombinant enzyme Gibbs,M.D.;R.A.Reeves;A.Sunna;P.L.Bergquist https://doi.org/10.1007/s002849900471
  5. Biotechnol. Appl. Biochem. v.26 β-Agarase from Pseudomonas sp. W7: purification of the recombinant enzyme from Escherichia coli and the effects of salt on its activity Ha,J.C.;G.T.Kim;S.K.Kim;T.K.Oh;J.H.Yu;I.S.Kong
  6. Agri. Biol. Chem. v.35 A simple activity measurement of lysozyme Imoto,T.;K.Yagishita https://doi.org/10.1271/bbb1961.35.1154
  7. J. Appl. Microbiol. v.84 Isolation and characterization of an active mannanase-producing anaerobic bacterium, Clostridium tertium KT-5A, from lotus soil Kataoka,N.;Y.Tokiwa https://doi.org/10.1046/j.1365-2672.1998.00349.x
  8. J. Microbiol. Biotechnol. v.12 Isolation of phytase-producing Pseudomonas sp. and optimization of its phytase production Kim,Y.H.;M.N.Gwon;S.Y.Yang;T.K.Park;C.G.Kim;C.W.Kim;M.D.Song
  9. Bergeys Manual of Systematic Bacteriology Krieg,N.R.;J.G.Holt
  10. MEGA: Molecular Evolutionary Genetics Analysis(Version 1.01) Kumar,S.;K.Tamura;N.Nei
  11. Algal physiology and biochemistry Cell wall and intercellular region polysaccharides Mackie,W.;R.D.Preston;Stewart,W.P.D.(ed.)
  12. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller,G.L. https://doi.org/10.1021/ac60147a030
  13. Eur. J. Biochem. v.133 Porphyran primary structure Morrice,L.M.;M.W.MacLean;W.F.Long;F.B.Williamson https://doi.org/10.1111/j.1432-1033.1983.tb07516.x
  14. Hydrobiologia v.204 Antitumor activity of marine algae Noda,H.;H.Amano;K.Arashima;K.Nishizawa https://doi.org/10.1007/BF00040290
  15. Fish. Sci. v.60 Study on antihypertensive and antihyperlipidemic effects of marine algae Ren,D.;H.Noda;H.Amano;T.Nishino;K.Nishizawa
  16. J. Biol. Chem. v.195 Note on sugar determination Somogyi,M.
  17. Methods Enzymol. v.160 Method for measuring cellulose activities Thomas,M.W.;K.M.Bhat https://doi.org/10.1016/0076-6879(88)60109-1
  18. Nucl. Acids Res. v.76 CLUSTAL W: Improving the sensitivity of progressive multiple sequence weighing, position-specific gap penalties and weight matrix choice Thomson,J.D.;D.G.Higgins;T.J.Gibson
  19. Biosci. Biotechnol. Biochem. v.57 Activation of murine macrophages by polysaccharide fractions from marine algae (Porphyra yezoensis) Yoshizawa,Y.;A.Enomoto;H.Todoh;A.Ametani;S.Kaminogawa https://doi.org/10.1271/bbb.57.1862
  20. Annu. Rev. Microbiol. v.50 Microbial hydrolysis of polysaccharides Warren,R.A.J. https://doi.org/10.1146/annurev.micro.50.1.183