Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Opuntia ficus-indica on Lipidperoxide and cholesterol level

Opuntia ficus-indica가 과산화지질 및 콜레스테롤에 미치는 영향

  • Published : 2003.12.01
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Abstract

Opuntia ficus-indica is widely used as folk medicine for burned wound, edema and indigestion, etc. After the adaptation of rats for seven days, OF (200mg/kg) was administered into rats intraperitoneally every day for two weeks. On the 14th day, $3.3m\ell$ of $CCl_4$ (50% $CCl_4$ : Olive oil = 1 : 1) was treated to rats and on the 15th day, they were operated. We measured the levels of total cholesterol, HDL-cholesterol, LDL-cholesterol, total lipid and triglyceride (TG) in serum and Malondialdehyde (MDA) in liver tissue. Opuntia ficus-indica and $CCl_4$-treated group (OFC) significantly decreased lipid peroxidation by 32.6% compared to $CCl_4$-treated abnomal group (CTA). Total lipid and TC of OFC administered group were lower than those of CTA group. Cholesterol levels showed significant effect in OFC group. Total cholesterol and LDL-cholesterol in OFC administered group were decreased by 12.7% and 17.4%, respectively, compared to those of CTA group. HDL-cholesterol in OFC administration group was increased by 19.7% compared to CTA group. This finding suggests that lipid peroxidation was inhibited in the significant level when $CCl_4$-induced rats were treated with OF. Based on this analysis, further study to identify the active components in this sample is warranted.

본 연구는 손바닥 선인장 투여 후 급성 $CCl_4$ 로 처리한 흰쥐의 간과 혈청을 사용하여, total cholesterol, HDL-cholesterol, LDL-cholesterol, total lipid, triglyceride (TG)를 간조직중에서 MDA를 측정함으로써 과산화지질 활성도와 콜레스테롤의 양을 조사하였다 OFC군은 손바닥 선인장 200mg/kg을, CTA 군은 saline을 2주간 매일 투여한 다음 간 손상의 유도는 사염화탄소를 olive oil에 1:1 비율로 용해시켜 체중 1kg당 50% $3.3m\ell$ 의용량을 복강으로 해부하기 24시간 전에 반 투여, 12시간 전에 나머지 반 투여하였다. 그 후 10시간 절식 후 혈액과 간을 채취하여 관찰하였다. 간 조직 중의 과산화지질 함량은 CTA 군은 NCT 군에 대하여 31.9% 증가를 보였으나, OFC 군에서는 CTA 군에 대해 32.6% 감소하는 경향을 나타내었다. 혈중 total lipid와 TG의 양은 손바닥 선인장을 투여한 군 (OFC) 이 CTA 군에 비해 각각 16.8%, 52.3%의 유의성 있는 감소를 보였다. Total cholesterol과 LDL-cholesterol은 OFC 군이 CTA 군에 대해 각각 12.7% 17.4% 정도로 크게 감소되는 것으로 나타났다. HDL-cholesterol은 CTA 군이 NCT 군에 대해 42.8% 감소하였으나, 손바닥 선인장을 투여함으로써 CTA 군에 대해 19.7% 증가를 나타냈다. 동맥경화지수는 CTA 군이 NCT 군에 비해 3.6배 높고, OFC 군이 CTA 군에 비해 1.8배 낮은 것으로 나타났다. 이상의 결과로, 손바닥 선인장을 투여한 후 간 독성을 유발하였을 때 과산화지질 및 콜레스테롤이 유의적인 수준으로 억제되었다.

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