Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Changes of Enzyme Activities and Inorganic Nutrient Contents Associated with Flower Development in Tulip (Tulipa gesneriana)

튤립(Tulipa gesneriana) 꽃의 발달단계에 따른 효소 활성 및 미량요소 함량의 변화

  • 조효경 (대구가톨릭대학교 자연대학 생명자원학부) ;
  • 박순기 (경북대학교 농과대학 농학과) ;
  • 정일경 (대구가톨릭대학교 자연대학 생명자원학부) ;
  • 이재석 (대구가톨릭대학교 자연대학 생명자원학부)
  • Published : 2003.12.01
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Abstract

This study was carried out to investigate the changes of enzymes and micro inorganic nutrients that is associated with flower senescence during flower development in tulip cultivars, ‘Apeldoorn’ and ‘Golden Apeldoorn’. Ribonuclease, peroxidase and protease activities were gradually increased from the stage of early flowering to later Polyphenol oxidase showed the highest activity at stage 5, which the flower was in full bloom indicating that it acts at an initial stage of flower senescence. The protease activity was different in the petal extracts during flower development between the cultivars ‘Apeldoorn’ (red petal) and ‘Golden Apeldoorn’ (yellow petal). This result suggested that protease might relate to pigment biosynthesis in petal of tulip. In contrast to the decrease of inorganic nutrients K, Mn, Zn and P contents during floral development, Ca, Mg and Fe showed the gradual increasement that is similar with ribonuclease, peroxidase and protease. It suggests that they have some interaction during flower senescence.

본 실험은 튤립 품종 ‘Apeldoorn’과 ‘Golden Apeldoorn’의 꽃잎 발달 단계에 따른 각종 가수분해 효소 활성의 변화 및 효소 활성의 조절에 관여하는 무기성분들의 함량의 변화를 조사하였다. RNase와 peroxidase, protease의 효소활성은 초기단계로부터 꽃이 발달이 진행될수록 증가하는 경향을 보였다. Polyphenol oxidase의 경우, 꽃이 만개한 5단계에서 활성이 가장 높았으며, 이 효소가 노화가 시작되는 초기 단계에 관여하는 것으로 생각된다. 한편, Protease의 활성은 ‘Apeldoorn’과 ‘Golden Apeldoorn’의 꽃 발달동안 꽃잎 추출물에서 현저한 차이가 관찰되었다 이 결과는 protease는 튤립의 화색의 생합성에 관련성을 시사한다. 조사된 K, Mn, Zn, P의 함량은 꽃 발달 동안 점차적으로 감소하는 경향을 보인 반면, Ca, Mg, Fe의 무기성분은 초기 단계에서 꽃의 노화 단계까지 점차적으로 증가하였으며, RNase와 peroxidase, protease의 효소 활성의 변화와 같은 경향을 보였다. 이는 꽃의 발달단계동안 이들 효소와 무기성분 사이에 서로 결합력이 있다고 추측된다. 본 연구결과는 튤립 꽃의 발달단계에 따른 기초적인 생화학적 변화의 경향을 조사한 결과로서, 절화수명 연장을 위한 기초자료로 유용하게 활용될 수 있을 것이다.

Keywords

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