Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Protective Mechanism of Flavonoids Isolated from Rhus Vernicifiua on the Paraquat Toxicity Reducing Agent and its Inhibition Mechanism

옻나무 목부로부터 분리한 Favonoids가 Paraquat 독성의 경감기전 검색

  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated to elevate the modulatory effect of flavonoid(fustin, sulfuretin, 10 mg/kg) which was isolated from Rhus verniciflua Stokes(RVS) in male Sprague-Dawley rats for 2 weeks on the toxicity of paraquat. In the flavonoids pretreated groups, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, blood urea nitrogen, creatinine, malondialdehyde and alkaline phosphatase activity in serum and malondialdehyde, alkaline phosphatase activity and collagen in lung tissue which was induced paraquat toxicity were slightly decrease compared to the normal group. In the lung tissue of flavonoids pretreated groups, malodialdehyde value, G-6-phosphatase activity and collagen synthesis were recovered to tile normal values and alkaline phosphatase activity was increased. From these results, we concluded that flavonoids which were isolated from RVS is an effective agent to inhibit the pulmonary and internal toxicities and hence we concluded that acitive components of fustin and sulfuretin which were isolated from RVS might be removed free radicals induced by paraquat.

옻나무(Rhus verniciflua Stokes)로부터 제초제인 paraquat(PQ)의 독성 경감기전을 추구할 목적으로 ethylacetate 분획에서 분리한 fustin 및 sulfuretin을 실험동물에 투여하고서 혈액학적 변화 및 간장 중 활성산소에 미치는 영향 검토한 결과 혈액 생화학적 인자 분석을 종합해 보면 옻나무의 메탄을 엑스(250 mg/kg), 에틸아세테이트 엑스(250 mg/kg), fustin(10 mg/kg) 및 sulfuretin(10 mg/kg)을 2주간 각각 경구투여 하고서 PQ를 투여하므로서 PQ의 독성이 유의성 있게 억제되었다. in vivo에서 옻나무의 분획 및 성분의 투여는 혈액생화학적 검사에서 PQ에 의해 유도된 간독성 지표인 s-GPT, s-GOT치, 신장독성지표인 BUN, creatinine치 및 조직손상의 지표인 ALP, MDA치를 억제하였다 폐조직중의 MDA함량, ALP활성 및 collagen함량도 억제하였다. 이로써 옻나무에는 PQ의 폐독성 및 각 장기의 독성을 효과적으로 경감시켜 줄 수 있는 물질이 함유되어 있는 것으로 사료된다.

Keywords

References

  1. Clinical chemistry : Interpretation and Techniques Alex,K.;L.S.Laverne
  2. Arch. of Toxicol. v.77 no.6 Consecutive administration of paraquat to rats induces enhanced cholesterol peroxidation and lung injury Adachi,J.;K.Ishii;M.Tomita;T.Fujita;Y.Nurhantari;Y.Nagasaki;Y.Ueno
  3. Life Sci. v.14 Reduction of paraquat toxicity by superoxidase dismutase Autor,A.P. https://doi.org/10.1016/0024-3205(74)90439-1
  4. The International Journal of Biochemistry v.13 no.4 Studies on lipid peroxidation in rat liver by copper deficiency Balevska,P.S.;E.M.Russanov;T.A.Kassabova https://doi.org/10.1016/0020-711X(81)90122-1
  5. Toxicol. Appl. Pharmacol. v.35 A mechanism of paraquat toxicity in mice and rats Bus,J.S.;S.Z.Cagen;M.Olgaard;J.E.Gibson https://doi.org/10.1016/0041-008X(76)90073-9
  6. Arch. Biochem. Biophys. v.306 no.1 Studies on the inhibition of collagen synthesis in fibroblasts treated with paraquat Darr,D.;S.Comb;S.Murad;S.Pinnel https://doi.org/10.1006/abbi.1993.1510
  7. Biochem. Biophys. Acta. v.1502 no.3 Antioxidant properties of colchicine in acute carbon tetrachloride induced rat liver injury and its role in the resolution of established cirrhosis Das,D.;P.W.Pemberton;P.C.Burrows;C.Gordon;A.Smith;R.F.McMahon;T.W.Warnes https://doi.org/10.1016/S0925-4439(00)00059-4
  8. Am. J. Physiol. v.228 Pulmonary effect of paraquat in the first day atrer injection Fisher,H.K.;J.A.Clements;D.F.Tierney;R.R.Wrigh
  9. J. Biol. Chem. v.66 The colorimetric determination of phosphorus Fiske,G.H.;Y.Subbarow
  10. Life Science v.65 Increased myofibroblasts contractile sensitivity in paraquat pretreated rat lung tissue Hemmati,A.A.;R.Hicks https://doi.org/10.1016/S0024-3205(99)00499-3
  11. Pharmacological Research: The official Journal of the Italian Pharmacological Society v.46 no.3 The role of sodium cromolyn in treatment of paraquat-induced pulmonary fibrosis in rat Hemmati,A.A.;Z.Nazari;M.E.Motlagh;S.Goldasteh https://doi.org/10.1016/S1043-6618(02)00122-6
  12. J. Biochem. v.83 no.5 Purification and characterization of alkaline phosphatase from plasma membranes of rat ascites hepatoma Ikehara,Y.;K.Mansho;K.Takahashi;K.Kato
  13. Anal. Biochem. v.112 A simple method to determine nanogram levels of 4-hydroxyproline in biological tissue Jamall,I.S.;V.N.Finelli https://doi.org/10.1016/0003-2697(81)90261-X
  14. Toxicol. Appl. Pharmacol. v.17 Toxicity of paraquat to rats and its effect of rat lungs Kimbrough,R.D.;T.B.Gaines
  15. J. Clin. Pathol. v.7 Estimation of plasma phosphate by determination of hydrolyzed phenol with amino antipyrine Kind,P.R.N.;E.J.King https://doi.org/10.1136/jcp.7.4.322
  16. Clin. Biochem. v.34 no.2 The evaluation of lipid peroxidation and adenosine deaminase activity in patients with Behcet's disease Kose,K.;C.Yazici;O.Assioglu https://doi.org/10.1016/S0009-9120(01)00190-4
  17. Am. J. Clin. Med. v.28 no.1 Antioxidant and hepatoprotective effects of Anoectochilus formosanus and Gynostemma pentaphyllum Lin,C.C.;P.C.Huang;J.M.Lin https://doi.org/10.1142/S0192415X00000118
  18. J. Biol. Chem. v.193 Protein Measurement with folin phenol reagent Lowry,O.H.;N.J.Rosebrough;A.L.Farr;R.J.Rendall
  19. J. Biol. Chem. v.256 Paraquat and NADPH dependent lipid peroxidation in lung microsome Misra,H.P.;L.D.Gorsky
  20. Fundam. Clin. Pharmacol. v.3 Prevention of $CCl_4$-induced liver cirrhosis by silymarin Mourelle,M.;P.Muriel;L.Favari;T.Franco https://doi.org/10.1111/j.1472-8206.1989.tb00449.x
  21. Anal. Biochem. v.95 Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction Ohkawa,H.;N.Ohishi;K.Yaki https://doi.org/10.1016/0003-2697(79)90738-3
  22. Vet. Hum. Toxicol. v.26 A literature review of paraquat toxicity Onyeama,H.P.;F.W.Oehme
  23. Med. Sci. Monit. v.7 no.6 Role of reactine oxygen species(ROS) in patients with erythema migrans, an early manifestation of Lyme borreliosis Pancewicz,S.A.;E.Skrzydlewska;T.Hermanowska-Szpakowicz;J.M.Zajkowska;M.Kondrusik
  24. Biochem. Pharmacol. v.31 Effect of diabetes on rat liver cytochrome P-450 Past,M.R.;D.E.Cook https://doi.org/10.1016/0006-2952(82)90569-X
  25. Amer. J. Pathol. v.28 A colorimetric method for the detemination of serum glutamic oxaloacetic and glutamic pyruvic transaminase Reitman,S.;S.K.Frankel
  26. Clin. Toxicol. v.18 Paraquat poisoning. Toxicologic and pathologic findings in three fatal cass Russell,L.A. https://doi.org/10.3109/15563658108990319
  27. Exp. Lung. Res. v.21 no.6 Taurine protects rat bronchioles from acute ozone-induced lung inflammation and hyperplasia Schuller,G.B.;R.E.Gordon;E.Park;K.J.Pendino;D.L.Laskin https://doi.org/10.3109/01902149509031768
  28. Am. J. Med. v.32 A clinical appraisal of the plasma concentration and endogenous clearance of creatinine Doolan,P.D.;E.L.Alpen;G.B.Theil https://doi.org/10.1016/0002-9343(62)90183-3
  29. Toxicol. Appl. Pharmacol. v.20 Differences in the potentiation of carbon tetrachloride in rats by ethanol isopropanol pretreatment Traiger,G.J.;G.L.Plaa https://doi.org/10.1016/0041-008X(71)90094-9
  30. Biochem. Pharm. v.37 The accumulation and locallisattion od putrescine, spermidine and paraquat in rat lung. In vitro and in vivo studies Wyatt,A.R.;M.F.Soames https://doi.org/10.1016/0006-2952(88)90536-9
  31. Chemistry and Physics of Lipids v.45 Lipid peroxides and human disease Yagi,K. https://doi.org/10.1016/0009-3084(87)90071-5
  32. Ph. D. Thesis, Kyungsung University Pharmacological Evaluation on Antiinflammatory Effects of the Flavonoids Isolated from the Heartwood of Rhus verniciflua Stokes Yoon,B.J.

Cited by

  1. Antioxidative Activities of Rhus verniciflua Bark from Different Area vol.25, pp.3, 2012, https://doi.org/10.9799/ksfan.2012.25.3.430
  2. Effects of Dietary Resveratrol on Growth Performance, Blood Biochemical Parameter, Immunoglobulin, and Blood Antioxidant Activity in Broiler Chicks vol.42, pp.2, 2015, https://doi.org/10.5536/KJPS.2015.42.2.147