KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Enzymatic Decolorization of Various Dyes by Trametes versicolor KCTC 16781

Trametes versicolor KCTC 16781에 의한 다양한 염료의 색도제거 특성

  • 박철환 (한국생산기술연구원 청정설계기술팀) ;
  • 이유리 (한국생산기술연구원 청정설계기술팀) ;
  • 김탁현 (한국생산기술연구원 청정설계기술팀) ;
  • 이명구 (한국생산기술연구원 청정설계기술팀) ;
  • 이병환 (한국생산기술연구원 청정설계기술팀) ;
  • 이진원 (광운대학교 화학공학과) ;
  • 김상용 (한국생산기술연구원 청정설계기술팀)
  • Published : 2003.10.01
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Abstract

Due to the low biodegradability of dyes, conventional biological wastewater treatment systems are inefficient in treating dye wastewater. Various white-rot fungi were investigated for the decolorization of six industrial dyes (reactive blue 5, reactive blue 16, reactive black 5, acid black 52, reactive orange 16, and acid violet 43). Among ten fungi, T. versicolor KCTC 16781 was selected as a testing strain because this had the best performance of decolorization for six dyes from the results of the solid culture experiments. In liquid culture medium, T. versicolor KCTC 16781 decolorized over 96% of six dyes for 48 hrs. Laccase started to produce in the early stage of the culture, and showed the highest peak value of 2.3 U/mL in 24 hrs. Enzyme activity remained constant until the end of culture. Fungal decolorization is a promising alternative to replace or supplement present treatment process.

고체배양을 통해 10종의 균주 중에서 선별된 고활성 균주인 T. versicolor KCTC 16781은 100 mg/L의 6가지 염료(reactive blue 19, reactive blue 49, reactive black 5, acid black 52, reactive orange 16, and acid violet 43)를 15일 이내에 효율적으로 분해하였다. T. versicolor KCTC 16781은 액체배양에서 반응 2-3일 이내에 6가지 염료의 색도를 100% 제거시켰으며, 배양 초기에 빠른 속도로 효소를 생산하여 높은 활성을 보였다. 이로 인해 색도제거 속도가 기존의 연구에 보고된 결과들보다 우수함을 입증하였다. 서로 다른 종류의 염료분해 실험에서, 효소활성이 반응 초기에 작은 차이를 보였지만, 최대 효소활성에 도달한 후의 값이 2.0$\pm$0.5 U/mL의 범위에 들어 염료의 종류에 관계없이 비슷한 범위의 활성을 보이는 것을 확인할 수 있었다. 염료의 색도제거가 완벽하게 이루어진 후에도 효소활성은 감소하지 않았으며, 배양 말기까지 2.0 U/mL 이상의 효소활성을 유지하여 고농도의 염료를 제거할 수 있는 가능성을 보였다.

Keywords

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