Journal of Plant Biotechnology

  • 제30권2호
  • /
  • Pages.195-199
  • /
  • 2003
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Streptanthus tortus 배양세포에서 미생물 Elicit가 사부형성에 미치는 영향

Effect of the Elicit of Microorganism on the Formation of Phloem in Suspension Cultures of Streptanthus tortus

  • 조봉희 (수원대학교 자연과학대학 생명과학과, 기능성생명소재 연구소)
  • Cho, Bong-Heuy (Department of Life Science, Center for smart Bio-Materials, the University of Suwon)
  • 발행 : 2003.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Extracts of Escherichia coli as a elicit were treated to suspension cultures of Streptanthus tostus in order to observe the effect on the pholem development. By the elicit treatment, cell wall, sieve endoplasmic reticulum (SER) and p-protein were normally synthesized, but the structure of amyloplast was changed from a round form to irregular and swollen unhalthy form with a tiny starch granular. Oil drops were new synthesized and accumulated in a large oleoplast and proteins were also accumulated in a single membrane. The concentration of sucrose in the phloem, which was induced during the elicit treatment, was higher than normally developed phloem cells. These results suggest that phloem cells might be changed in the normal cycles of metabolism of lipids, carbohydrates and proteins to overcome during the eilicit stress.

키워드

참고문헌

  1. Cho BH (1987) Analysis of the low affinity system of the uptake of fructose in suspension culture cells. Kor J Bot 30: 277-285
  2. Cho BH (1988) Effects of 2,4-D on phloem formation and differentiation of Streptanthus cells in suspension culture, Kor J Plant Tiss Cult 15: 217-223
  3. Cho BH (1992) The role of sugars on phloem formation in Streptanthus suspension cultured cells. Kor J Piant Tiss Cult 19: 205-208
  4. Cho BH (1996) Phloem differentiation in cell culture of Streptanthus tortuss. Kor J Plant Tiss Cult 23: 107-111
  5. Cho BH (1998) Isolation of phloem cell and active transport of sucrose by isolated phloem and parenchyma cells of Streptanthus tortus suspension culture. Kor J Plant Tiss Cult 25: 7-11
  6. Greenberg JT, Guo A, Klessig DF, Ausubel FM (1994) Programmed cell death in plants: A pathogen-triggered response activated coordinately wtth multiple defense functions. Cell 77: 551-563 https://doi.org/10.1016/0092-8674(94)90217-8
  7. Hengariner MO, Ellis RE, Horvitz HR (1992) Caenorhabditis elegans gene Ced-9 protects cells from programmed cell death. Nature 358: 494-499
  8. Hon WC, Griffith M, ,Mlynarz A, Kwok YC, Yang DCC (1995) Antifreeze proteins in winter rye are similar to pathogenesis-related proteins. Plant Physiol 109: 879-889 https://doi.org/10.1104/pp.109.3.879
  9. Horvitz HR,Ellis HM, Sternberg PW (1982) Programmed cell death in nematode development. Neurosci Comment 1: 56-65
  10. Lee SH, Crae HS, Lee TK, Kim SH, Shin SH, Cho BH, Cho SH, Kang BG, Lee WS (1998) Ethylene-mediated phospholipid catabolic pathway in glucose-starved carrot suspension cells. Plant Fhysiol 116: 223-229 https://doi.org/10.1104/pp.116.1.223
  11. Malamy J, Henning J, Klessig DF (1992) Temperature dependent induction of salicylc acid and its conjugated during the resistance response to tobacco mosaic virus infections. Plant Cell 4: 359-366 https://doi.org/10.1105/tpc.4.3.359
  12. Moriyasu Y, Oasumi E (1996) Autophage in Tobacco suspension cultured cells in response to sucrose starvation. Plant Physiol 111: 1233-1241
  13. Murashige RD, Skoog E (1962) A revised medium for rapid growth and bioassay wtth tabacco tissue cultures. Plant Fhysiol 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Parkin KL, Kuo SJ (1989) Chilling-induced lipid degradation in cucumber (Cucumis sativa L. cv Hybrid C) fruit. Plant Physiol 90: 1045-1056
  15. Senaratma T, Mackay CE, Mckersie BD, Fletcher RA (1988) Uniccoazole-induced chilling tolerance in tomato and its relationship to antioxidant contains. J Plant Physiol 133: 56-61 https://doi.org/10.1016/S0176-1617(88)80084-1
  16. Singh NK, Nelson DE, Kuhn D, Hasegawa PM, Bressan RA (1989) Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential. Plant Physiol 90: 1096-1101 https://doi.org/10.1104/pp.90.3.1096
  17. Tomei SD, Cope FO (1991) Apoptosis of the molecular basis of cell death. Cold Spring Harber Laboratory Press, pp 193-246
  18. Van Handel E (1968) Direct microdetermination of sucrose. Anal Biochem 22: 280-283 https://doi.org/10.1016/0003-2697(68)90317-5
  19. Wang Q, Monroe J, Sjolund R (1995) Identification and characterization of a phloem-specific $\beta$-amylase. Plant Physiol 109: 743-750 https://doi.org/10.1104/pp.109.3.743
  20. Wolf T, Ready DF (1991) Cell death in normal and rough eye mutants of Drosophila. Development 113: 825-839