DOI QR코드

DOI QR Code

Geminivirus L4 유전자 도입에 따른 형질전환 애기장대의 감염유사증상 분석

Pseudosymptom Analysis Induced by Geminivirus L4 Gene in Transgenic Arabidopsis

  • 발행 : 2003.06.01

초록

The factors for symptom development caused by Beat curly top virus(BCTV) have been analyzed by using a molecular genetic approach based on expressing BCTV encoded proteins in transgenic plants. BCTV open reading frame (ORF) L4 expression in transgenic Arabidopsis resulted in abnormal plant development and the production of callus inflorescence stems and bumpy trichomes, confiming that this gene alone is a primary symptom determinant. The L4 gene expression by northern hybridization in transgenic plants and a range of phenotypes were analyzed.

키워드

참고문헌

  1. Bechtold N, PelletierG (1998) In planta Agrobacterium-mediated transfonnation of adult Arabidopsis thaliana plants by vacuum infiltration. Methods Mol BioI 82: 259-66
  2. Bennett CW (1971) The curly top disease of sugarbeet and other plants. St. Paul, Minnesota, Am Phytopathol Soc
  3. Davis KR, Schott E, Ausubel FM (1991) Virulence of selected phytopathogenic pseudomonads in Arabidopsis thaliana. Mol Plant Microbe Interact 4: 477-488 https://doi.org/10.1094/MPMI-4-477
  4. Hormuzdi SG, Bisaro DM (1993) Genetc analysis of beet curly top virus: Evidence for three virion sense genes involved in movement and regulation of single-and doule-stranded DNA levels. Virology 193: 900-909 https://doi.org/10.1006/viro.1993.1199
  5. Lazarowttz SG (1992) Geminiviruses: Genome structure and gene function. Crit Rev Plant Sci 11: 327-349 https://doi.org/10.1080/07352689209382350
  6. Lee S (1995) Genetic and molecular analysis of interactions between Arabidopsis and beet curly top virus. PhD thesis,The Ohio State University, Columbus
  7. LeeS, Stenger DC,Bisaro DM, Davis KR. (1994) Identification of loci in Arabidopsis that confer resistance to geminivirus infection. Plant J 6: 525-535 https://doi.org/10.1046/j.1365-313X.1994.6040525.x
  8. Lee S, Park JB (1996) Auxin effects on symptom development of beet curly top virus infected Arabidopsis thaliana. J Plant BioI 39: 449-456
  9. Pascal E, Sanderfoot AA, Ward BM, Medville R, Turgeon R, Lazarowitz SG (1994) The geminivirus BR1 movement protein binds single-stranded DNA and localizes to the cell nucleus. Plant Cell 6: 995-1006 https://doi.org/10.1105/tpc.6.7.995
  10. RigdenJE, Krake LR, RezainanMA,DrylB (1994) ORF C4 of Tanato Leaf Curl Geminivirus Is a determinant of Symptom Severity. Virology 204: 847-850 https://doi.org/10.1006/viro.1994.1606
  11. Stanley J (1991) The molecular detenninans of geminivirus pathogenesis. Sem Virol2: 139-149
  12. Stanley J, Latham JR, Pinner MS, Bedfird I, Markham PG (1992) Mutational analysis of the monopariitegeminivirus beet curly top virus. Virology 191: 396-405 https://doi.org/10.1016/0042-6822(92)90201-Y
  13. Stanley J, Markham PG, Callis RJ, Pinner MS(1986) The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO J 5: 1761-1767
  14. Stenger DC (1994) Complete nucleotide sequence of the hypervirulent CFH strain of beet curly top virus. Mol Plant-Microbe Interact 7: 2211-2215
  15. Stenger DC, Carbonaro D, Duffus JE (1990) Genomic characterization of phenotypicvariants of beet curly top virus. J Gen Virol 71: 2211-2215 https://doi.org/10.1099/0022-1317-71-10-2211