Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation of Alginate-Degrading Marine Bacteria and Characterization of Alginase

알긴산 분해 해양미생물의 분리 및 alginase 특성 평가

  • 이재화 (신라대학교 공과대학 생명공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과)
  • Published : 2003.10.01
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Abstract

Various marine microorganisms were isolated from seaweed, and their alginate-degrading activities were investigated. An alginate-degrading bacteria, Vibrio sp. AEBL-211, showed highest level of alginase activity when cultured on a mineral salt medium containing 0.7%(w/v) sodium alginate as the sole carbon source. The intracellular alginase from AEBL-211 was partially purified by ion chromatography on DE 52-cellulose column and gel filteration on Sepacryl G-200 column, and showed guluronate-specific 1yase activity.

다양한 종류의 미역으로부터 알긴산 분해 활성을 가지는 해양 미생물인 Vibrio sp. AEBL-211을 분리하였다. 우선, 알긴산 고체배지에서의 halo 크기가 크고 환원당 생성량이 가장 많은 해양 미생물을 선발하고, 생화학 및 영양적인 특성 분석 결과 등을 바탕으로 Vibro 속으로 동정하였다. DE 52-cellulose 및 Sephacryl G-200를 이용한 음이온 교환 및 gel permeation chromatography를 통해 부분 정제된 alginase 효소액을 얻었다. 부분 정제된 alginase 효소를 이용하여 guluronic acid에 대한 mannuronic acid의 비율이 다른 sodium alginate 시료에 대한 분해능을 평가한 결과, guluronic acid의 상대적 양이 많은 알긴산에 대한 활성이 상대적으로 우수하여 Vibrio sp. AEBL-211 유래의 alginase는 guluronic acid가 많은 G-rich block에 대한 분해활성이 높은 특성을 가지는 것으로 판단되었다.

Keywords

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