생명과학회지 (Journal of Life Science)

  • 제13권5호
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  • Pages.712-717
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  • 2003
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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박테리오신 leucocin A를 생산하는 Saccharomyces serevisiae 세포의 제작

Establishment of a Leucocin A Producing Sccharomyces cerevisiae Cell

  • 이상현 (신라대학교 생명공학과, 마린 바이오 산업화 지원센터)
  • 발행 : 2003.10.01
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초록

박테리오신의 일종인 leucocin A를 생산하는 효모의 제작을 위하여 117 bp 길이의 개시코돈과 종지코돈을 포함하는 leucocin A 유전자를 합성하여 효모운반체인 pAUR123에 클로닝하였다. 형질전환된 효모는 부패균의 일종인 고초균(Bacillus subtilis)에 대해 항균활성을 나타냈다. 형질전환 효모로부터 분리한 플라스미드를 주형으로 하고 leucocin A에 특이적인 primer들을 이용하여 PCR 반응을 행한 결과, 효모에 도입된 leucocin A 유전자를 확인할 수 있었다. 이 연구의 결과로 부패하기 쉬운 식품들의 보존성을 향상시키거나, 내성이 생긴 병원균의 생육을 저해하기 위한 항생제로 사용할 수 있는 박테리오신을 산업적으로 생산할 수 있는 효모세포를 제작하였다.

In order to establish yeast cells that produce leucocin A, a bacteriocin, the 117 bp leucocin A gene with start and stop codons was synthesized and cloned in pAUR123, a yeast vector. Transformed yeast cells showed antibacterial activity against Bacillus subtilis. The leucocin A gene was confirmed by means of PCR methods with plasmid prepared from transformed yeast cells as template and two leucocin A-specific primers. In this results, yeast cells that produce mass amounts of bacteriocin to use as food preservative or antibiotics were established.

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