생명과학회지 (Journal of Life Science)

  • 제13권5호
  • /
  • Pages.705-711
  • /
  • 2003
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

Pseudomonas elodea ATCC 31461에 의한 gellan 생산의 최적 배양조건

Optimization of culture condition for the gellan production by Pseudomonas elodea ATCC 31461

  • Lim, Sung-Mi (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Wu, Jian-Rong (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Lee, Jin-Woo (Division of Biotechnology, College of Natural Resources & Life Science, Dong-A University) ;
  • Kim, Sung-Koo (Department of Biotechnology and Bioengineering, Pukyong National University)
  • 발행 : 2003.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Pseudomonas elodea ATCC 31461에 의해 생산되는 미생물 다당류인 gellan의 최적 조건을 확립하기 위해 flask상에서 inoculum size, 탄소원 종류 및 농도, 질소원 종류, C/N ratio등을 조사하였다. Gellan 생산을 위한 최적 inoculum size는 5%(v/v)로 나타났으며, 1.0%(w/v)의 glucose에서 2.22의 최대 specific yield 와 $0.03 g/\ell$의 생산성을 얻을 수 있었다. 또한 ammonium nitrate가 없을 때 최대 생산을 얻을 수 있어, gellan 생산을 위해서 nitrogen limitation이 필요함을 알 수 있었다 Glucose와 bactopeptone을 이용하여 C/N ratio를 조사한 결과 20에서 gellan 생산이 가장 높게 나왔다.

The gellan was produced by Pseudomonas elodea under aerobic condition. In this study, the effects of inoculum size, carbon sources and concentration, nitrogen source, and C/N ratio on the cell growth and the production of gellan were evaluated. The maximum growth of P. elodea and gellan production was obtained at 5% (v/v) of inoculum size and glucose showed best results among 9 carbon sources tested. The maximum specific yield of 2.22 and productivity of $0.03 g/\ell$h were obtained at 1.0% (w/v) of glucose. The maximum gellan production was obtained at medium without ammonium nitrate. This indicates that nitrogen limitation is essential for the production of gellan. The highest cell and gellan production were obtained at 20 of C/N ratio.

키워드

참고문헌

  1. J. Appl. Bacteriol. v.62 A bacterium yielding a polysaccharide with unusual properties Anson,A.;P.J.Fisher;A.F.K.Kennedy;I.W.Sutherland https://doi.org/10.1111/j.1365-2672.1987.tb02392.x
  2. Recent Development in Industrial Polysaccharides: Biomedical and Biotechnological Advances Crescenzi,V.;I.C.M.Dea;S.Paoletti;S.Stivala;I.W.Sutherland
  3. Carbohydr. Polym. v.32 Rapid purification of commercial gellan gum to highly soluble and gellable monovalent cation salts Doner,L.W. https://doi.org/10.1016/S0144-8617(96)00168-3
  4. Anal. Chem. v.28 Colorimetirc method for determination of sugars and related substances Dubois,M.;K.A.Gilles;J.K.Hamilton;P.A.Rebers;F.Smith https://doi.org/10.1021/ac60111a017
  5. J. Biotechol. v.34 Effect of the dissolved oxygen tension during cultivation of Xanthomonas campestris on the production and quality of xanthan gum FloresF.;L.G.Torres;E.Galindo https://doi.org/10.1016/0168-1656(94)90086-8
  6. Agric. Biol. Chem. v.29 Production of a new acidic polysaccharide succinoglucan by Alcaligenes faecalis var. myxogees Harada,T.;T.Yoshimura;H.Hidaka;A.Koreeda https://doi.org/10.1271/bbb1961.29.757
  7. Manual of Industrial Microbiology and Biotechnology Inoculum development Hunt,G.R.;R.W.Stieber
  8. Carbohydr. Res. v.124 Structural studies of gellan gum, an extracellular polysaccharide elaborated by Pseudomonas elodea Jansson,P.E.;B.Lindberg https://doi.org/10.1016/0008-6215(83)88361-X
  9. US patent 4,326,053 Polysaccharide S-60 and bacterial fermentation process for its fermentation Kang,K.S.;G.T.Veeder
  10. US patent 4,377,633 Fermentation process for preparation of polysaccharide S-60 Kang,K.S.;G.T.Veeder
  11. Appl. Environ. Microbiol. v.43 Agar-like polysaccharide produced by a Pseudomonas species: production and basic properties Kang,K.S.;G.T.Veeder;P.J.Mirrasoul;T.Kaneko; I.W.Cottrell
  12. Microbial Technology v.1 Chapter 13 Polysaccharide Kang,K.S.;I.W.Cottrell;Peppler,H.J.(ed.);D.Perlman(ed.)
  13. Carbohydr. Res. v.156 Identification and location of glycerate, an unusual acyl substituent in gellan gum Kuo,M.S.;A.J.Mort;D.Anne https://doi.org/10.1016/S0008-6215(00)90109-5
  14. Biotechnol. Appl. Biochem. v.14 Gellan gum biosynthetic enzyme in producing and nonproducing variants of Pseudomonas elodea Ligio,O.M.;I.S.Correia
  15. Appl. Environ. Microbiol. v.47 Gelrite as a gelling agent in media for the growth of thermophilic microorganisms Lin,C.C.;L.E.Casida
  16. Ph.D. thesis, University of Strathclyde Expolysaccharide elaboration by the polymorphic fungus Aureobasidium pullulans Madi,N.S.
  17. Letters Appl. Microbiol. v.23 Production and rheological characteristics of the microbial polysaccharide gellan Manna,B.;A.Gambhir;P.Ghosh
  18. Carbohydr. Polym. v.41 Texture properties of high and low acyl mixed gellan gels Mao,R.;J.Tang;B.G.Swanson https://doi.org/10.1016/S0144-8617(99)00108-3
  19. Appl. Microbiol. v.44 Production of extracellular polysaccharide by Zoogloea ramigera Noberg,A.B.;S.O.Enfors
  20. Carbohydr. Res. v.124 Structure of the acidic extracellular gelling polysaccharide produced by Pseudomonas elodea O'Neill,M.A.;R.R.Selvendran;V.J.Morris https://doi.org/10.1016/0008-6215(83)88360-8
  21. J. Gen. Microbiol. v.139 Gellan-related polysaccharides and the genus Sphingomonas Pollock,T.J. https://doi.org/10.1099/00221287-139-8-1939
  22. Food Technol. v.37 Laboratoryproduced microbial polysaccharide has many potential food applications as a gelling, stabilizing, and texturizing agent Sanderson,G.R.;R.C.Clark
  23. Appl. Envrion. Microbiol. v.37 Nutritional studies on xanthan production by Xanthomonas campestris NRRL B1459 Souw,P.;A.L.Demain
  24. Trends Biotechnol. v.16 Novel and established applications of microbial polysaccharides Sutherland,I.W. https://doi.org/10.1016/S0167-7799(97)01139-6
  25. Food hydrocolloids: stucture, properties and function Gellan gum production and properties Vendrusculo,C.T.;J.L.Pereira;A.R.P.Scamparini;Nishinari,K.(ed.);E.Doi(ed.)
  26. Microbios. v.94 Effect of complex nitrogen sources upon gellan production by Sphingomonas paucimobilis ATCC 31461 West,T.P.;B.Strohfus
  27. J. Gen. Microbiol. v.102 Exopolysaccharide production by Pseudomonas NCIB 1264 grown in batch culture Williams,A.G.;J.W.P.Wimpenny https://doi.org/10.1099/00221287-102-1-13
  28. Kor. J. Appl. Microbiol. Biotechnol. v.12 Study on the production of pullulan by Aureobasidium pullulans Yim,M.H.;H.S.Son;N.H.Chung;H.C.Yang
  29. Korea J. Food Sci. Technol. v.19 Polysaccharide production by Pseudomonas elodea ATCC 31461 Yoo,J.Y.;D.H.Shin;D.H.Chung

피인용 문헌

  1. Impact of glycerol and nitrogen concentration on Enterobacter A47 growth and exopolysaccharide production vol.71, 2014, https://doi.org/10.1016/j.ijbiomac.2014.04.012