생명과학회지 (Journal of Life Science)

  • 제13권5호
  • /
  • Pages.699-704
  • /
  • 2003
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

리스테리아균의 특성분석을 위한 Molecular Typing 방법의 상호보완

Enhanced Discrimination of Listeria spp. Using RAPD Fingerprinting Complemented by Ribotyping-PCR

  • 발행 : 2003.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

리스테리아를 보다 효과적으로 typing할 수 있는 방법을 찾기 위해 표준균주 13종을 대상으로 하여 RAPD, ERIC (Enterobacterial repetitive intergenic consensus) fingerprinting, ribotyping-PCR의 분리력을 비교해 보았다. DG107 (primer 6) 또는 DG122 (Lis 11) primer를 이용한 RAPD의 경우 11가지의 유형으로 분류되는 반면, ERIC fingerprinting은 9가지, ribotyping-PCR은 7가지씩의 유형을 보였다. 그러나 2가지 primer를 이용하여 각각 행한 RAPD 결과를 종합하거나, DG122를 이용한 RAPD와 ribotyping-PCR의 결과를 종합할 경우 13가지의 유형으로 모두 분리할 수 있었다.

The results typed by random amplification of polymorphic DNA (RAPD) were compared with those obtained by Enterobacterial repititive intergenic consensus (ERIC) fingerprinting and ribotyping-PCR. The discriminatory power of RAPD typing was the best among the methods tested. RAPD typing with two different primers for 13 Listeria spp. reference strains produced 11 patterns each. In contrast, ERIC fingerprinting produced 9 patterns and ribotyping-PCR produced 7 patterns each. Composite of two separate RAPD (Lis 11 and primer 6) results or RAPD (Lis11)/ ribotyping-PCR differentiated all 13 Listeria spp. reference strains. Therefore, composite of 2 separate RAPD (Lis11 and primer 6) or composite of RAPD (Lis11)/ribotyping-PCR is expected the most promising approach for typing field isolated Listeria spp. strains.

키워드

참고문헌

  1. Molecular Epidemiology Molecular differentiation of bacterial strains Brown,E.;Carrinton,M.(ed.);A.R.Hoelzel(ed.)
  2. Food Microbiology Adams,M.R.;M.O.Moss
  3. FEMS Immunology and Medical Microbiol. v.29 Application of randomly amplified polymorphic DNA (RAPD) analysis for typing avian Salmonella enterica subsp. enterica Chansiripornchai,N.;P.Ramasoota;A.Bangtrakulnonth;J.Sasipreeyajan;S.B.Svenson https://doi.org/10.1111/j.1574-695X.2000.tb01526.x
  4. International J. Food Microbiol. v.84 Rapid enumeration of Listeria monocytogenes in milk using competitive PCR Choi,W.S.;C.H.Hong https://doi.org/10.1016/S0168-1605(02)00401-4
  5. International J. Food Microbiol. v.53 Listeria monocytogenes in pork slaughtering and cutting plants: use of RAPD,PFGE and PCR-REA for tracing and molecular epidemiology Giovannacci,I.;C.Ragimbeau;S.Queguiner;G.Salvat;J.L.Vendeuvre;V.Carlier;G.Ermel https://doi.org/10.1016/S0168-1605(99)00141-5
  6. J. Clin. Microbiol. v.26 Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity Hunter,P.R.;M.A.Gaston
  7. International J. Food Microbiol. v.76 Genetic homogenety among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes Jaradat,Z.W.;G.E.Schutze;A.K.Bhunia https://doi.org/10.1016/S0168-1605(02)00050-8
  8. J. Clin. Microbiol. v.30 Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping Kostman,J.R.;T.D.Elliot;J.J.Lipuma;T.L.Stull
  9. J. Microbiol. Methods v.34 A rapid differentiation of Listeria monocytogenes by use of PCR-SSCP in the listeriolysin O (hlyA) locus Lehner,A.;S.Loncarevic;M.;Wagner;J.Kreike;E.Brandl https://doi.org/10.1016/S0167-7012(98)00076-1
  10. J. FdHyg. Safety. v.18 Primers for typing Listeria spp. using random amplified polymorphic DNA (RAPD) analysis Lim,H.;C.H.Hong;G.J.Bahk;W.S.Choi
  11. International J. Food Microbiol. v.42 A rapid method for the identification and partial serotyping of Listeria monocytogenes in food by PCR and restriction enzyme analysis Manzano,M.;L.Cocolin;C.Cantoni;G.Comi https://doi.org/10.1016/S0168-1605(98)00086-5
  12. FEMS Immunol. Medical Microbiol. v.14 Comparison of random amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus-PCR for epidemiological studies of Salmonella Millemann,Y.;M.C.Lesage-Descauses;J.P.Lafont;E.Chaslus-Dancla https://doi.org/10.1111/j.1574-695X.1996.tb00279.x
  13. Food Microbiology v.17 Identification of Listeria monocytogenes contamination sources in two fresh sauce production plants by pulse-field gel electrophoresis Pourshaban,M.;M.Gianfranceschi;A.Gattuso;F.Menconi; P.Aureli https://doi.org/10.1006/fmic.1999.0329
  14. Method in Microbiology v.13 Serotyping of Listeria monocytogenes Seeliger,H.P.R.;K.Hohne;Bergan,T.(ed.);J.R.Norris(ed.) https://doi.org/10.1016/S0580-9517(08)70372-6
  15. Food Microbiol. v.16 A rapid PCR-based hybridization assay for the detection of Listeria monocytogenes in channel catfish Wang,C.;C.Hong https://doi.org/10.1006/fmic.1998.0237