Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Pine Needle Ethyl Acetate Fraction on Membrane Fluidity and Oxidative Stress in Brain Membranes of Rats

뇌 세포막의 유동성과 산화적 스트레스에 미치는 솔잎(Pine Needle) 에틸아세테이트획분의 영향

  • Published : 2003.10.01
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Abstract

This study was designed to investigate the effects of ethyl acetate (EtOAc) fraction of pine (Pinus densiflora Sieb et Zucc) needle on membrane fluidity (MF), basal and induced oxygen radical (BOR and IOR), lipid peroxide (LPO) and oxidized protein (OP) as a oxidative stress, and lipofuscin (LF) in brain membranes of Sprague-Dawley (SD) rats. Male SD rats were fed basic diets (control) and experimental diets (EtOAc-25, EtOAc-50 and EtOAc-100) for 45 days. MF was significantly increased (about 10%) in mitochondria of EtOAc-100 group. BOR and IOR formations in mitochondria were significantly inhibited (about 9∼10% and 17∼24%, respectively) in EtOAc-50 and EtOAc-100 groups, while BOR and IOR formations in microsomes were significantly inhibited (about 12∼17% and 12∼16%, respectively) in EtOAc-50 and EtOAc-100 groups compared with control group. LPO levels in mitochondria and microsomes were significantly inhibited (about 9∼l2% and 12∼19%, respectively) in EtOAc-50 and EtOAc-100 groups, whereas significant difference between OP or LF levels and control group in these membranes could not be obtained. These results suggest that administrations of ethyl acetate fraction of pine needle may play an effective role in an attenuating an oxidative stress and in increasing membrane fluidity.

뇌 세포막 유동성의 촉진 및 LPO생성의 억제효과는 중풍 등 뇌혈관질환을 억제할 수 있을 뿐만 아니라 혈관성치매의 예방에도 크게 기여할 것으로 기대된다[26,18]. 따라서 솔잎 EtOAc획분을 하루 25, 50, 100 mg/kg BW로써 SD계 랫트에 45일동안 투여하여 뇌조직중의 세포막 유동성 (membrane fluidity : MF), 기초 및 유도활성산소(BOR 및 IOR), 산화적 스트레스로서 과산화지질(lipid peroxide : LPO) 및 산화단백질(oxidized protein : OP), 그리고 리포푸신(lipofuscin : LF)의 축적에 미치는 영향을 평가하여 보았다. 솔잎 EtOAc획분의 투여에 의하여 뇌조직의 mitochondria 및 microsome획분에서 용량의존적으로 MF가 증가효과가 나타났지만, EtOAc-100 투여그룹의 mitochondria 획분에서만 약 10%의 유의적인 MF의 증가효과가 인정되었다. 뇌조직의 mitochondria에서 기초 및 유도활성산소로서 BOR 및 IOR의 생성은 EtOAc-50 및 EtOAc-100투여그룹에서 각각 9.2∼10.2% 및 17.1∼24.0%의 유의적인 억제효과가 인정되었고, microsomes에서 BOR 및 IOR의 생성은 EtOAc-50및 EtOAc-100투여그룹에서 각각 11.6∼16.6% 및 12.1∼16.1%의 유의적인 억제효과가 인정되었다. 산화적 스트레스에 미치는 영향은 mitochondria 및 microsomes획분에서 EtOAc-50 및 EtOAc-100투여그룹에서 각각 8.5∼12.0% 및 11.6∼18.5%의 유의적인 LPO의 생성 억제효과가 인정되었지만, 뇌조직의 두 획분에서 OP의 생성은 EtOAc획분의 용량의존적으로 억제되었지만, 유의성은 인정할 수 없었다. 또한 뇌조직에서 LF의 축적도 EtOAc획분의 용량 의존적으로 억제효과가 나타났지만, 유의성은 인정할 수 없었다. 이상의 결과에서 평가하여 볼 때 솔잎 EtOAc획분의 투여는 뇌조직의 유동성을 촉진하고 LPO의 생성을 효과적으로 억제할 수 있지만, 현재의 투여농도에서는 OP 및 LF의 생성을 방지할 수 없을 것으로 생각된다.

Keywords

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