생명과학회지 (Journal of Life Science)

  • 제13권5호
  • /
  • Pages.684-691
  • /
  • 2003
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

간장 세포막의 유동성과 산화적 스트레스에 미치는 솔잎(Pine Needle) 에틸아세테이트획분의 영향

Effects of Pine Needle Ethyl Acetate Fraction on Membrane Fluidity and Oxidative Stress in Liver Membranes of Rats

  • 발행 : 2003.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

솔잎 EtOAc획분을 하루 25, 50, 100 mg/kg체중으로서 SD계 랫트에 45일동안 투여하여 간장획분의 세포막 유동성(membrane fluidity MF), 기초 및 유도활성산소(BOR 및 IOR), 산화적 스트레스로서 과산화지질(lipid peroxide : LPO) 및 산화단백질(oxidized protein : OP), 그리고 리포푸신(lipofuscin : LF)의 축적에 미치는 영향을 평가하여 보았다. 솔잎 EtOAc획분의 투여에 의하여 간장의 mitochondria 및 microsome획분에서 어느 정도 MF가 증가하였지만, 유의성은 EtOAc-100 투여그룹의 mitochondria획분에서만 인정되었다. 기초 및 유도활성산소로서 BOR 및 IOR의 생성 억제효과는 간장의 두 획분의 EtOAc-50 및 EtOAc-100투여그룹에서 유의적인 활성산소의 생성 억제 효과가 인정되었다. 산화적 스트레스에 미치는 영향에서는 mitochondria획 분에서 는 EtOAc-100투여그룹에서, 그리고 microsome획분에서는 EtOAc-50 및 EtOAc-100투여그룹에서 유의적인 LPO의 생성 억제효과가 인정되었고, mitochondria 획분에서는 EtOAc-50 및 EtOAc-100투여그룹에서 유의적인 OP의 생성 억제효과가 인정되었지만, microsome 획분에서는 아무런 유의성도 인정할 수 없었다. 그렇지만, 간장의 클로로포름층에서 측정한 LF의 축적은 EtOAc-25, EtOAc-50 및 EtOAc-100 투여그룹에서 다같이 유의적인 LF의 축적 억제효과가 인정되었다. 이상의 결과에서 평가하여 볼 때 솔잎 EtOAc획분의 투여는 간장의 유동성을 촉진하고 산화적 스트레스를 효과적으로 억제할 수 있을 것으로 기대된다.

This study was designed to investigate the effects of ethyl acetate (EtOAc) fraction of pine (Pinus densiflora Sieb et Zucc) needle extract on membrane fluidity (MF), basal and induced oxygen radical (BOR and IOR), lipid peroxide(LPO) and oxidized protein (OP) as an oxidative stress, and lipofuscin(LF) in liver membranes of male Sprague-Dawley rats. Rats were fed basic diets (control) and experimental diets (EtOAc-25, EtOAc-50 and EtOAc-100) for 45days. MFs were significantly increased (about 10%) in mitochondria of EtOAc-100 group compared with control group. BOR and IOR formations in mitochondria were significantly inhibited (about 12∼18% and 9 ∼l2%, respectively) in EtOAc-50 and EtOAc-100 groups, while BOR and IOR formations in microsomes were significantly inhibited (about 9∼l3% and 18∼19%, respectively) compared with control group. LPO levels were significantly inhibited (about 10% and 12∼13%, respectively) in mitochondria of EtOAc-100 and microsomes of EtOAc-50 and EtOAc-100 groups, whereas OP levels were significantly inhibited (about 13∼14%) in mitochondria of EtOAc-50 and EtOAc.-100 groups compared with control group. LF formations were significantly inhibited (about 10∼14%) in these three EtOAc groups. These results suggest that ethyl acetate fraction of pine needle may play an effective role in attenuating an oxidative stress and increasing a membrane fluidity.

키워드

참고문헌

  1. Age v.12 The effect of food restriction on kidney membrane structures of aging rats Choi,J.H.;B.P.Yu https://doi.org/10.1007/BF02432350
  2. Age v.13 Unsuitability of TBA test as a lipid peroxidation marker due to prostaglandin synthesis in the aging kidney Choi,J.H.;B.P.Yu https://doi.org/10.1007/BF02432391
  3. Kor. J. Gerontol. v.4 no.2 Studies on age-related physiological changes in brain of senescence accelerated mouse(SAM) Choi,J.H.;B.P.Yu
  4. Free Rad. Biol. & Med. v.18 no.2 Brain synaptosomal aging : Free radicals and membrane fluidity Choi,J.H.;B.P.Yu https://doi.org/10.1016/0891-5849(94)00106-T
  5. Age v.19 Analysis of liped composition and hydroxyl radicals in brain membranes of senecence-accelerated mice Choi,J.H.;J.I.Kim;D.W.Kim;Y.S.Moon;H.Y.Chung;B.P.Yu https://doi.org/10.1007/BF02439515
  6. J. Nutr. Health & Aging v.2 no.3 The effects of dietary restriction on age-related changes in rat serum prostaglandins Choi,J.H.;B.P.Yu
  7. J. Nutr. Health & Aging v.2 no.3 Dietary restriction as a modulator of age-related changes in rat kidney prostagladin production Choi,J.H.;B.P.Yu
  8. J. Nutr. Health & Aging v.2 no.3 Modulation of age-related alterations of iron, ferritin, and lipid peroxidation in rat brain synaptosomes Choi,J.H.;D.W.Kim;B.P.Yu
  9. Age & Nutrition v.10 no.1 The effcets of dietary restriction on age-related changes in rat serum prostaglandins Choi,J.H.;B.P.Yu
  10. Korean J. Life Science v.7 no.4 Effect of pine needle extract (PNE) on physiological activity of SD rats. I. Feeding effect of PNE on lipid and oxygen radical metabolisms in serum of SD rats Choi,J.H.;D.W.Kim;J.H.Kim;K.S.Kim;J.S.Lee
  11. Korean J. Life Science v.8 no.1 Effect of pine needle extract (PNE) on physiological activity of SD rats Ⅱ. Feeding effect of PNE on oxygen radicals and their scavenger enzymes in brain membranes of SD rats Choi,J.H.;J.H.Kim;D.W.Kim;K.S.Kim;J.S.Lee;Y.H.Baek
  12. Korean J. Life Science v.8 no.2 Effect of pine needle extract (PNE) on physiological activity of SD rats Ⅲ. Feeding effect of PNE on fluidity and neurotransmitter-related enzymes in brain membranes of SD rats Choi,J.H.;J.H.Kim;D.W.Kim;C.H.Hwang;D.I.Kim;J.S.Lee
  13. Anal. Biochem. v.52 Measurement of fluorescent lipid peroxidation products in biological systems and tissues Fletcher,B.L.;C.J.Dillard;S.A.L.Tappel https://doi.org/10.1016/0003-2697(73)90327-8
  14. Proc. Natl. Acad. Sci. v.12 Lipid fluidity markedly modulates the binding of serotonin to mouse brain membranes Heron,D.S.;M.Shinitzky;M.Hershkowitz;D.Samuel
  15. J. Korean Soc. Food Nutr. v.25 no.3 Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet Kang,Y.H.;Y.K.Park;T.Y.Ha;K.D.Moon
  16. J. Korean Soc. Food Nutr. v.25 no.3 Effects of pine needle extracts on enzyme activities of serum and liver, and liver morphology in rats fed high fat diet Kang,Y.H.;Y.K.Park;T.Y.Ha;K.D.Moon
  17. Korean J. Food Sci. Technol. v.30 no.1 Cytotoxic effect of the pine needle extract Kim,E.J.;S.W.Jung;K.P.Choi;S.S.Ham;H.Y.Kang
  18. Kor. J. Gerontol. v.1 no.1 Effect of dietary supplementation with pine leaf on lipid parameters in rats Kim,J.D.;T.H.Yoon;M.Choi;K.J.Im;J.S.Ju;S.Y.Lee
  19. Mutat. Res. v.347 no.3 Study on the antimutagenic effect of pine needle extract Kong,Z.;Z.Liu;B.Ding https://doi.org/10.1016/0165-7992(95)00026-7
  20. Biochem. & Biophysic, Res. Com. v.163 no.2 Perturbations in cerebral oxygen radical formation and membrane order following vitamin E deficiency Lebel,C.P.;I.N.Odunze;A.Jr.;S.C.Bondy https://doi.org/10.1016/0006-291X(89)92301-2
  21. Methods Enzymol. v.186 Determination of carbony1 content in oxidatively modified proteins Levine,R.L.;D.Garland;C.N.Oliver;A.Amici;I.Ciment;A.G.Lenz;B.Ahn;S.Shaltiel;E.R.Stadtman https://doi.org/10.1016/0076-6879(90)86141-H
  22. J. Biol. Chem. v.193 Protein measurement with the FolinPhenol reagent Lowry,O.H.;N.J.Roseborough;L.A.Farr;R.J.Randall
  23. Colored Illustrations of WAKANYAKU v.Ⅱ Namba,T.
  24. Principles and procedures of stastistics Steel,R.G.D.;J.H.Torrie
  25. Soc. Exp. Biol. Med. v.193 Mechanism of food restriction: Protection of cellular homeostasis Yu,B.P.;D.W.Lee;C.G.Marler;J.H.Choi https://doi.org/10.3181/00379727-193-42982
  26. Free Rad. Biol. Med. v.21 Aging and oxidative stress : Modulation by dietary restriction Yu,B.P. https://doi.org/10.1016/0891-5849(96)00162-1