Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Screening of anti-candidiosis agent from medicinal and wild plants

Candidiosis 치료제 개발을 위한 약용 및 야생 식물의 항진균 활성의 검색

  • 손호용 (안동대학교 식품영양학과) ;
  • 금은주 (안동대학교 식품영양학과) ;
  • 권윤숙 (안동대학교 식품영양학과) ;
  • 권기석 (안동대학교 생명자원과학부) ;
  • 진익렬 (경북대학교 미생물학과) ;
  • 권하영 (안동대학교 식품영양학과) ;
  • 권정숙 (안동대학교 식품영양학과) ;
  • 손건호 (안동대학교 식품영양학과)
  • Published : 2003.10.01
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Abstract

Candida albicans is one of the most common etiological agents in fungi-associated skin infections. There is an increase of candidiosis especially in the patient of acquired or induced immunodeficiency syndromes or in the event of long-term antibiotics and immuno-suppressor or cytotoxic therapies. To screen out reliable and effective anti-candidiosis agent, in this study, we have evaluated antifungal activity of 298 plant extracts against C. albicans. Based on the results of disc-paper method and determination of minimal inhibitory concentration, fifteen extracts were finally selected as possible sources of anti-candidiosis agent. Especially, six different plant extracts, such as Rubus parvifolius, Euphorbia pekinensis, Coptis chinensis, Eugenia aromaticum, Paeonia lactiflora var. hortensis and Paeonia suffruticosa showed strong antifungal activity against C. albicans, not to S. cerevisiae. These results suggested that medicinal and wild plants could be the potential source of antifungal agent.

안전성이 확보된 식용, 또는 약용식물로부터 항캔디다 활성이 우수한 항진균 후보물질을 개발하기 위해, 298종의 천연물로부터 추출부위 및 추출 용매를 달리하여 384종의 추출물을 제조하였다. 제조된 추출물의 항캔디다 활성을 disc-paper를 이용한 생육 저지환의 유무 및 크기 판정과 microbroth dilution법을 이용하여 C. albicans KCTC 1940과 S. cerevisiae IFO 0233를 대상으로 평가하였다. 그 결과 20종 식물로부터 조제된 25종의 추출물이 우수한 항캔디다 활성을 나타냄을 확인하였고, 그 중에서도 멍석딸기, 정향, 천황련, 작약, 모란, 대극잎 추출물은 강력 한 항캔디다 활성$(MIC=50 \mug/m\ell)$을 보였다. 특히, 멍석딸기(에틸아세테이트 추출물), 정향(부탄올 추출물), 호장근(에틸아세테이트 추출물), 작약(부탄올 추출물), 대극잎(메탄올 추출물)의 경우에는 C. albicans에 높은 선택독성을 나타내어, 안정성이 확보된 식물 추출물로부터 새로운 진균 감염증 치료제 개발이 가능함을 제시하였다.

Keywords

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