Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Assessment of Genetic Relationship among Curcurbitaceae Cultivars Revealed by RAPD Marker

RAPD Marker에 의한 호박의 품종간 유연 관계 분석

  • 김창훈 (농림부 국립종자관리소 재배시험과) ;
  • 이승인 (농림부 국립종자관리소 재배시험과) ;
  • 유병천 (농림부 국립종자관리소 재배시험과) ;
  • 송인호 (농림부 국립종자관리소 재배시험과) ;
  • 권용삼 (농림부 국립종자관리소 재배시험과)
  • Published : 2003.10.01
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Abstract

The objective of this study was to assess of genetic variation within and between pumpkin species including Cucurbita maxima, C. moschata, C. pepo and C. maxima${\times}$C. moschata using RAPD markers. The 16 primers showed the amplification of 136 scorable fragments ranging from about 100 bp to 2300 bp. A total of 94 DNA fragments were polymorphic with an average 5.9 polymorphic bands per primer. A species $(C. maxima\timesC. moschata)$ has the highest number of polymorphic loci. Based on obtained data, UPGMA cluster analysis was conducted. Twenty pumpkin cultivars were classified into three large categories and identified genetic distance of cluster ranging from 0.38 and 1.00. Clustering was in accordance with the division of Curcurbitaceae into four species, C. maxima, C. moschata, C. pepo and C. $C. maxima\timesC. moschata$. Therefore, RAPD method may be essential tool for enabling discrimination of pumpkin cultivars.

Keywords

References

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